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I2BR71 (I2BR71_PSEFL) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL AFJ56754.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:PflA506_0689 EMBL AFJ56754.1
OrganismPseudomonas fluorescens A506 [Complete proteome] EMBL AFJ56754.1
Taxonomic identifier1037911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 348204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding175 – 23056ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3021Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I2BR71 [UniParc].

Last modified July 11, 2012. Version 1.
Checksum: 6D742F833231EBA5

FASTA36539,098
        10         20         30         40         50         60 
MSKVRVGIIF GGRSAEHEVS LQSARNIVDA LDRSRFEPVL IGIDKAGHWH LNDTSNFLLN 

        70         80         90        100        110        120 
QENPALIALN QSNRELAVVP GKASQQVVET SGQGVLEHID VIFPIVHGTL GEDGCLQGLL 

       130        140        150        160        170        180 
RMADLPFVGS DVLGSAVCMD KDISKRLLRD AGIAVTPFIT LTRANAARTS FETAAGKLGV 

       190        200        210        220        230        240 
PMFVKPANQG SSVGVSKVGS EAEYRAAVEL ALGFDEKVLV ESAVQGREIE CAVLGNDQPI 

       250        260        270        280        290        300 
ASGCGEIVVS SGFYSYDSKY IDGQAAQVVV PAAISHDASE RIRSLAIEAF EVLGCAGLAR 

       310        320        330        340        350        360 
VDVFLTQDGE VLINEINSLP GFTRISMYPK LWQAAGMSYS ELVSRLIELA LERYAGRKGL 


KINRQ 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003041 Genomic DNA. Translation: AFJ56754.1.
RefSeqYP_006322219.1. NC_017911.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFJ56754; AFJ56754; PflA506_0689.
GeneID12962193.
KEGGpfc:PflA506_0689.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPFLU1037911:GLIS-688-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI2BR71_PSEFL
AccessionPrimary (citable) accession number: I2BR71
Entry history
Integrated into UniProtKB/TrEMBL: July 11, 2012
Last sequence update: July 11, 2012
Last modified: June 11, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)