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I1YGJ2 (I1YGJ2_METFJ) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Q7C_866 EMBL AFJ02035.1
OrganismMethylophaga frappieri (strain ATCC BAA-2434 / DSM 25690 / JAM7) [Complete proteome] [HAMAP] EMBL AFJ02035.1
Taxonomic identifier754477 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeMethylophaga

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain110 – 305196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding136 – 19156ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2591Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2741Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I1YGJ2 [UniParc].

Last modified July 11, 2012. Version 1.
Checksum: 8EF104D6912C490A

FASTA31234,034
        10         20         30         40         50         60 
MMDQITDPKA FGRVAVLMGG RSAEREISLK SGNAVLSALL AKGVEAVGID VDSHIDITLR 

        70         80         90        100        110        120 
EGQFDRAFIV LHGRGGEDGE IQGLLQAMAI PFTGSGVTGA MLSMNKRISK LIWQQQALST 

       130        140        150        160        170        180 
PKFRLVNAET DPQELVIALG LPLVIKPVHE GSSIGMSKVT DGEAIAAAIR LALQFDQDVI 

       190        200        210        220        230        240 
AEQWINGAEY TASILAGNAL PVIRLKTPRE FYDFEAKYHA NNTEYLCPCG LTEEQEKRCQ 

       250        260        270        280        290        300 
ALALDAFNAL QMKGWGRVDV MADQQGNFYL LEANGVPGMT DHSLVPMAAR ETGLDFQQLC 

       310 
WRILETSLEE KN 

« Hide

References

[1]"Complete genome sequences of Methylophaga sp. strain JAM1 and Methylophaga sp. strain JAM7."
Villeneuve C., Martineau C., Mauffrey F., Villemur R.
J. Bacteriol. 194:4126-4127(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JAM7 EMBL AFJ02035.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003380 Genomic DNA. Translation: AFJ02035.1.
RefSeqYP_006292722.1. NC_017856.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFJ02035; AFJ02035; Q7C_866.
GeneID12843204.
KEGGmec:Q7C_866.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycMSP754477:GLGI-863-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI1YGJ2_METFJ
AccessionPrimary (citable) accession number: I1YGJ2
Entry history
Integrated into UniProtKB/TrEMBL: July 11, 2012
Last sequence update: July 11, 2012
Last modified: June 11, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)