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Protein
Submitted name:

Translation elongation factor Tu

Gene

tuf

Organism
Methylophaga nitratireducenticrescens (strain ATCC BAA-2433 / DSM 25689 / JAM1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotationImported

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSP754476:GLGH-2309-MONOMER.
MSP754476:GLGH-2322-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Translation elongation factor TuImported
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:Q7A_2324Imported, Q7A_2337Imported
OrganismiMethylophaga nitratireducenticrescens (strain ATCC BAA-2433 / DSM 25689 / JAM1)Imported
Taxonomic identifieri754476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeMethylophaga
ProteomesiUP000009144 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

KOiK02358.
OMAiDEGGRHN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I1XL59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAKFERTK PHVNVGTIGH VDHGKTTLTA AITKVMAEAS GGEFKNYADI
60 70 80 90 100
DNAPEERERG ITIATAHVEY ETANRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVVNAAD GPMPQTREHI LLSRQVGVPY IIVYLNKADM VDDAELIELV
160 170 180 190 200
EMEVRELLDS YDFPGDDTPI IVGSALKALE GDTSEIGAPS IMRLAEAMDE
210 220 230 240 250
YFPEPKRAID GAFLMPIEDV FSISGRGTVV TGRVERGIVK VGDELEIVGI
260 270 280 290 300
KDTQKTTCTG VEMFRKLLDQ GQAGDNVGVL LRGTKREDVD RGQVLAHPGT
310 320 330 340 350
IKPHTKFEAE VYILSKDEGG RHTPFFNGYR PQFYFRTTDV TGACELPSGV
360 370 380 390
EMVMPGDNVK MDVTLINPIA MEEGLRFAIR EGGRTVGAGV VSKIVE
Length:396
Mass (Da):43,178
Last modified:July 11, 2012 - v1
Checksum:iBC7DE4736E52212C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003390 Genomic DNA. Translation: AFI85128.1.
CP003390 Genomic DNA. Translation: AFI85140.1.
RefSeqiWP_014707495.1. NC_017857.1.
YP_006296773.1. NC_017857.1.
YP_006296785.1. NC_017857.1.

Genome annotation databases

EnsemblBacteriaiAFI85128; AFI85128; Q7A_2324.
AFI85140; AFI85140; Q7A_2337.
KEGGimej:Q7A_2324.
mej:Q7A_2337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003390 Genomic DNA. Translation: AFI85128.1.
CP003390 Genomic DNA. Translation: AFI85140.1.
RefSeqiWP_014707495.1. NC_017857.1.
YP_006296773.1. NC_017857.1.
YP_006296785.1. NC_017857.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFI85128; AFI85128; Q7A_2324.
AFI85140; AFI85140; Q7A_2337.
KEGGimej:Q7A_2324.
mej:Q7A_2337.

Phylogenomic databases

KOiK02358.
OMAiDEGGRHN.

Enzyme and pathway databases

BioCyciMSP754476:GLGH-2309-MONOMER.
MSP754476:GLGH-2322-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequences of Methylophaga sp. strain JAM1 and Methylophaga sp. strain JAM7."
    Villeneuve C., Martineau C., Mauffrey F., Villemur R.
    J. Bacteriol. 194:4126-4127(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JAM1Imported.
  2. "Methylophaga nitratireducenticrescens sp. nov. and Methylophaga frappieri sp. nov., isolated from the biofilm of the methanol-fed denitrification system treating the seawater at the Montreal Biodome."
    Villeneuve C., Martineau C., Mauffrey F., Villemur R.
    Int. J. Syst. Evol. Microbiol. 63:2216-2222(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: JAM1Imported.

Entry informationi

Entry nameiI1XL59_METNJ
AccessioniPrimary (citable) accession number: I1XL59
Entry historyi
Integrated into UniProtKB/TrEMBL: July 11, 2012
Last sequence update: July 11, 2012
Last modified: April 1, 2015
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.