I1WP44 (I1WP44_BURPE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Elongation factor Tu HAMAP-Rule MF_00118
Short name=EF-Tu HAMAP-Rule MF_00118

Gene names

Name:	tuf HAMAP-Rule MF_00118
ORF Names:	BP1026B_I3445 EMBL AFI68024.1, BP1026B_I3460 EMBL AFI68038.1

Organism

Burkholderia pseudomallei 1026b EMBL AFI68038.1

Taxonomic identifier

884204 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia pseudomallei

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118 RuleBase RU000325
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00118
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00118.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. HAMAP-Rule MF_00118 RuleBase RU000325

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm HAMAP-Rule MF_00118
Ligand	GTP-binding RuleBase RU000323 HAMAP-Rule MF_00118 Nucleotide-binding
Molecular function	Elongation factor HAMAP-Rule MF_00118 RuleBase RU000325 EMBL AFI68038.1
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity HAMAP-Rule MF_00118
	Nucleotide binding	81 – 85	5	GTP By similarity HAMAP-Rule MF_00118
	Nucleotide binding	136 – 139	4	GTP By similarity HAMAP-Rule MF_00118

Sequences

	Sequence		Length	Mass (Da)
	I1WP44 [UniParc]. Last modified July 11, 2012. Version 1. Checksum: 4A60D806CDFF494C	FASTA	396	42,991
	10 20 30 40 50 60 MAKEKFERTK PHVNVGTIGH VDHGKTTLTA AIATVLSAKF GGEAKKYDEI DAAPEEKARG 70 80 90 100 110 120 ITINTAHIEY ETANRHYAHV DCPGHADYVK NMITGAAQMD GAILVCSAAD GPMPQTREHI 130 140 150 160 170 180 LLARQVGVPY IIVFLNKCDM VDDAELLELV EMEVRELLSK YDFPGDDTPI IKGSAKLALE 190 200 210 220 230 240 GDKGELGEVA IMNLADALDT YIPTPERAVD GAFLMPVEDV FSISGRGTVV TGRVERGVIK 250 260 270 280 290 300 VGEEIEIVGI KATAKTTCTG VEMFRKLLDQ GQAGDNVGIL LRGTKREDVE RGQVLAKPGS 310 320 330 340 350 360 ITPHTHFTAE VYVLSKDEGG RHTPFFNNYR PQFYFRTTDV TGSIELPKDK EMVMPGDNVS 370 380 390 ITVKLIAPIA MEEGLRFAIR EGGRTVGAGV VAKIIE

References

[1]

Hayden H.H., Brittnacher M.J., Wu Z., Lim R., Sims E., Chang J., Fong C., Radey M., Rohmer L., Zhou Y., Gillett W., Haugen E., Bovee D., Jewett D., McMonagle E., Guenthner D., Levy R., Olson M.V. , Miller S.I., Kaul R., Jacobs M.A. Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 1026b EMBL AFI68038.1.

[2]

"Evolution of Burkholderia pseudomallei in Recurrent Melioidosis." Hayden H.S., Lim R., Brittnacher M.J., Sims E.H., Ramage E.R., Fong C., Wu Z., Crist E., Chang J., Zhou Y., Radey M., Rohmer L., Haugen E., Gillett W., Wuthiekanun V., Peacock S.J., Kaul R., Miller S.I., Manoil C., Jacobs M.A. PLoS ONE 7:E36507-E36507(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 1026b EMBL AFI68038.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002833 Genomic DNA. Translation: AFI68024.1. CP002833 Genomic DNA. Translation: AFI68038.1.
RefSeq	YP_006276422.1. NC_017831.1. YP_006276436.1. NC_017831.1.
3D structure databases
ProteinModelPortal	I1WP44.
SMR	I1WP44. Positions 2-395.
ModBase	Search...
Proteomic databases
PRIDE	I1WP44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AFI68024; AFI68024; BP1026B_I3445. AFI68038; AFI68038; BP1026B_I3460.
GeneID	12831638. 12831653.
KEGG	bpz:BP1026B_I3445. bpz:BP1026B_I3460.
Phylogenomic databases
KO	K02358.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

I1WP44_BURPE

Accession

Primary (citable) accession number: I1WP44

Entry history

Integrated into UniProtKB/TrEMBL:	July 11, 2012
Last sequence update:	July 11, 2012
Last modified:	April 3, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)