D-alanine--D-alanine ligase - Burkholderia pseudomallei 1026b

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I1WFA6 (I1WFA6_BURPE) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 9. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047

Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047

Gene names

Name:	ddl HAMAP-Rule MF_00047 EMBL AFI64950.1
ORF Names:	BP1026B_I0284 EMBL AFI64950.1

Organism

Burkholderia pseudomallei 1026b EMBL AFI64950.1

Taxonomic identifier

884204 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group ›

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047 Contains 1 ATP-grasp domain. SAAS SAAS005905 HAMAP-Rule MF_00047

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation SAAS SAAS005905 HAMAP-Rule MF_00047 Peptidoglycan synthesis SAAS SAAS005905 HAMAP-Rule MF_00047
Cellular component	Cytoplasm SAAS SAAS005905 HAMAP-Rule MF_00047
Ligand	ATP-binding SAAS SAAS005905 HAMAP-Rule MF_00047 Magnesium SAAS SAAS005905 HAMAP-Rule MF_00047 Manganese SAAS SAAS005905 HAMAP-Rule MF_00047 Metal-binding SAAS SAAS005905 HAMAP-Rule MF_00047 Nucleotide-binding
Molecular function	Ligase SAAS SAAS005905 HAMAP-Rule MF_00047 EMBL AFI64950.1
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Domain

108 – 308

201

ATP-grasp By similarity HAMAP-Rule MF_00047

Nucleotide binding

138 – 193

ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding

262

Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047

Metal binding

275

Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047

Metal binding

275

Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Metal binding

277

Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence

Length

Mass (Da)

Tools

I1WFA6 [UniParc].

Last modified July 11, 2012. Version 1.
Checksum: CCD89397BEAE5E22
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FASTA

312

33,341

        10         20         30         40         50         60 
MSGIDPKRFG KVAVLLGGDS AEREVSLNSG RLVLQGLRDA GIDAHPFDPA QRPLAALKDE 

        70         80         90        100        110        120 
GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGIPTPP 

       130        140        150        160        170        180 
FETVMRGDDY AARAQDIVAK LGVPLFVKPA SEGSSVAVEK VKSADALPAA LEEAAKHDKI 

       190        200        210        220        230        240 
VIVEKSIEGG GEYTACIAAD LDLPLIRIVP AGEFYDYHAK YIANDTQYLI PCGLDAAKEA 

       250        260        270        280        290        300 
EFKRIARRAF DVLGCTDWGR ADFMLDAAGN PYFLEVNTAP GMTDHSLPPK AARAVGIGYS 

       310 
ELVVKVLSLT LD

« Hide

References

[1]

Hayden H.H., Brittnacher M.J., Wu Z., Lim R., Sims E., Chang J., Fong C., Radey M., Rohmer L., Zhou Y., Gillett W., Haugen E., Bovee D., Jewett D., McMonagle E., Guenthner D., Levy R., Olson M.V.

Jacobs M.A.
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 1026b EMBL AFI64950.1.

[2]

"Evolution of Burkholderia pseudomallei in Recurrent Melioidosis."
Hayden H.S., Lim R., Brittnacher M.J., Sims E.H., Ramage E.R., Fong C., Wu Z., Crist E., Chang J., Zhou Y., Radey M., Rohmer L., Haugen E., Gillett W., Wuthiekanun V., Peacock S.J., Kaul R., Miller S.I., Manoil C., Jacobs M.A.
PLoS ONE 7:E36507-E36507(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 1026b EMBL AFI64950.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002833 Genomic DNA. Translation: AFI64950.1.
RefSeq	YP_006273348.1. NC_017831.1.
3D structure databases
ProteinModelPortal	I1WFA6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AFI64950; AFI64950; BP1026B_I0284.
GeneID	12828208.
KEGG	bpz:BP1026B_I0284.
Phylogenomic databases
KO	K01921.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

I1WFA6_BURPE

Accession

Primary (citable) accession number: I1WFA6

Entry history

Integrated into UniProtKB/TrEMBL:	July 11, 2012
Last sequence update:	July 11, 2012
Last modified:	April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information