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Protein

Isocitrate dehydrogenase [NADP]

Gene

BANAN_03785

Organism
Bifidobacterium animalis subsp. animalis (strain ATCC 25527 / DSM 20104 / JCM 1190 / R101-8)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Sitei139 – 1391Critical for catalysisUniRule annotation
Sitei211 – 2111Critical for catalysisUniRule annotation
Metal bindingi251 – 2511Magnesium or manganeseUniRule annotation
Binding sitei259 – 2591NADPUniRule annotation
Metal bindingi274 – 2741Magnesium or manganeseUniRule annotation
Binding sitei327 – 3271NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPUniRule annotation
Nucleotide bindingi309 – 3146NADPUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciBANI703613:GL91-772-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:BANAN_03785Imported
OrganismiBifidobacterium animalis subsp. animalis (strain ATCC 25527 / DSM 20104 / JCM 1190 / R101-8)Imported
Taxonomic identifieri703613 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000009141 Componenti: Chromosome

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

KOiK00031.
OMAiQVIEHDV.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I1W9N9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIKVEGKV VELDGDEMTR VIWKDIKDRL ILPYLDIDLD YYDLGIENRD
60 70 80 90 100
ATDDQVTIDA AEAIKREHVG VKCATITPDE ARVEEFGLKK MWKSPNGTIR
110 120 130 140 150
NILGGTIFRE PIVIDNIPRL VPGWTKPIVV ARHAFGDQYK ATDFKVPTSG
160 170 180 190 200
TLTVTFTPND GSEPIEHVVF DYPGSGVAQV QYNLDESIRG FARACFNYGL
210 220 230 240 250
LRHYPVYLST KNTILKAYDG EFKDIFAEVF ETEYKQRYEQ EGLTYEHRLI
260 270 280 290 300
DDMVASSLKW HGGYVWACKN YDGDVQSDTV AQGFGSLGLM TSVLMTPDGQ
310 320 330 340 350
TVEAEAAHGT VTRHYRRWQK GEKTSTNPIA SIYAWTGGLK HRAKLDGTPQ
360 370 380 390 400
VANFAETLEQ TIISTVEGGQ MTKDLAILID PSHEWLDTEQ FMNALDENLQ

RNLAEQR
Length:407
Mass (Da):45,885
Last modified:July 11, 2012 - v1
Checksum:i518B7E387E8E21C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002567 Genomic DNA. Translation: AFI62971.1.
RefSeqiYP_006279934.1. NC_017834.1.

Genome annotation databases

EnsemblBacteriaiAFI62971; AFI62971; BANAN_03785.
KEGGibni:BANAN_03785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002567 Genomic DNA. Translation: AFI62971.1.
RefSeqiYP_006279934.1. NC_017834.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFI62971; AFI62971; BANAN_03785.
KEGGibni:BANAN_03785.

Phylogenomic databases

KOiK00031.
OMAiQVIEHDV.

Enzyme and pathway databases

BioCyciBANI703613:GL91-772-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Short communication: the complete genome sequence of Bifidobacterium animalis subspecies animalis ATCC 25527(T) and comparative analysis of growth in milk with B. animalis subspecies lactis DSM 10140(T)."
    Loquasto J.R., Barrangou R., Dudley E.G., Roberts R.F.
    J. Dairy Sci. 94:5864-5870(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25527 / DSM 20104 / JCM 1190 / R101-8Imported.

Entry informationi

Entry nameiI1W9N9_BIFAR
AccessioniPrimary (citable) accession number: I1W9N9
Entry historyi
Integrated into UniProtKB/TrEMBL: July 11, 2012
Last sequence update: July 11, 2012
Last modified: April 1, 2015
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.