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Protein

Allantoate deiminase 2

Gene

AAH2

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of purine nucleotides. Can use allantoate as substrate. The sequential activity of AAH, UGLYAH and UAH allows a complete purine breakdown without the intermediate generation of urea.1 Publication

Catalytic activityi

Allantoate + H2O = (S)-ureidoglycine + NH3 + CO2.1 Publication

Cofactori

Mn2+By similarity

Kineticsi

kcat is 34 sec(-1) for allantoate.1 Publication
  1. KM=102 µM for allantoate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi127Manganese 1By similarity1
    Metal bindingi136Manganese 1By similarity1
    Metal bindingi136Manganese 2By similarity1
    Metal bindingi173Manganese 2By similarity1
    Metal bindingi239Manganese 1By similarity1
    Metal bindingi457Manganese 2By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processPurine metabolism
    LigandManganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Allantoate deiminase 2Curated (EC:3.5.3.91 Publication)
    Alternative name(s):
    Allantoate amidohydrolase 21 Publication
    Short name:
    GmAAH21 Publication
    Gene namesi
    Name:AAH21 Publication
    Ordered Locus Names:Glyma09g05600Imported
    OrganismiGlycine max (Soybean) (Glycine hispida)Imported
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    Proteomesi
    • UP000008827 Componenti: Chromosome 9

    Subcellular locationi

    • Endoplasmic reticulum By similarity

    GO - Cellular componenti

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 30Sequence analysisAdd BLAST30
    ChainiPRO_500933637331 – 483Allantoate deiminase 2Sequence analysisAdd BLAST453

    Expressioni

    Tissue specificityi

    Expressed in stems and leaves, and at low levels in roots. Not detected in nodules.1 Publication

    Gene expression databases

    ExpressionAtlasiI1L153. baseline and differential.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3847.GLYMA09G05600.1.

    Structurei

    3D structure databases

    SMRiI1L153.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410II0M. Eukaryota.
    COG0624. LUCA.
    InParanoidiI1L153.
    KOiK02083.
    OMAiCDEEGTR.
    OrthoDBiEOG093608AX.

    Family and domain databases

    CDDicd03884. M20_bAS. 1 hit.
    InterProiView protein in InterPro
    IPR010158. Amidase_Cbmase.
    IPR001261. ArgE/DapE_CS.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    PfamiView protein in Pfam
    PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01879. hydantase. 1 hit.
    PROSITEiView protein in PROSITE
    PS00758. ARGE_DAPE_CPG2_1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    I1L153-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSATASNTF FLHSCFLLFC LLSAPSCVSM FSGIETGDLE KRDDLFPQIL
    60 70 80 90 100
    RDEAVARLYE LGKVSDASGY LERTFLSPAS MRAINLIRKW MEDAGLRTWV
    110 120 130 140 150
    DQMGNVHGRV DGANANAEAL LIGSHMDTVV DAGMFDGSLG IVSAISALKA
    160 170 180 190 200
    MHVNGKLQKL KRPVEVIAFS DEEGVRFQTT FLGSGAIAGI LPGTTLEISD
    210 220 230 240 250
    KREVMIKDFL KENSIDITEE SLLKLKYDPK SVWGYVEVHI EQGPVLEQVG
    260 270 280 290 300
    FPLGVVKGIA GQTRLKVTVR GSQGHAGTVP MSMRQDPMAA AAEQIVVLES
    310 320 330 340 350
    LCKHPEEYLS YDGHCSDSTV KSLSTSLVCT VGEISTWPSA SNVIPGQVTY
    360 370 380 390 400
    TVDIRAIDDL GREAVIYDLS KQIYQICDKR SVSCIIEHKH DAGAVICDSD
    410 420 430 440 450
    LSSQLKSAAY SALKKMEGDI QDEVPTLMSG AGHDAMAISH LTKVGMLFVR
    460 470 480
    CRGGISHSPQ EHVLDNDVWA ASLATLSFLE NLS
    Length:483
    Mass (Da):52,353
    Last modified:June 13, 2012 - v1
    Checksum:i7E9521B1AF259A49
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CM000842 Genomic DNA. Translation: KRH37194.1.
    RefSeqiXP_003533667.1. XM_003533619.3.
    UniGeneiGma.19265.

    Genome annotation databases

    EnsemblPlantsiGLYMA09G05600.1; GLYMA09G05600.1; GLYMA09G05600.
    GeneIDi100819399.
    GrameneiGLYMA09G05600.1; GLYMA09G05600.1; GLYMA09G05600.
    KEGGigmx:100819399.

    Similar proteinsi

    Entry informationi

    Entry nameiAAH2_SOYBN
    AccessioniPrimary (citable) accession number: I1L153
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2017
    Last sequence update: June 13, 2012
    Last modified: August 30, 2017
    This is version 40 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families