ID   I1KEN4_SOYBN            Unreviewed;       966 AA.
AC   I1KEN4;
DT   13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT   13-JUN-2012, sequence version 1.
DT   24-JAN-2024, entry version 61.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   Name=100807407 {ECO:0000313|EnsemblPlants:KRH55745};
GN   ORFNames=GLYMA_06G277500 {ECO:0000313|EMBL:KRH55745.1};
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847 {ECO:0000313|EnsemblPlants:KRH55745};
RN   [1] {ECO:0000313|EMBL:KRH55745.1, ECO:0000313|EnsemblPlants:KRH55745}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH55745};
RC   TISSUE=Callus {ECO:0000313|EMBL:KRH55745.1};
RX   PubMed=20075913; DOI=10.1038/nature08670;
RA   Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA   Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA   Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA   Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA   Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA   Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA   Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA   Stacey G., Shoemaker R.C., Jackson S.A.;
RT   "Genome sequence of the palaeopolyploid soybean.";
RL   Nature 463:178-183(2010).
RN   [2] {ECO:0000313|EnsemblPlants:KRH55745}
RP   IDENTIFICATION.
RC   STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH55745};
RG   EnsemblPlants;
RL   Submitted (FEB-2018) to UniProtKB.
RN   [3] {ECO:0000313|EMBL:KRH55745.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Callus {ECO:0000313|EMBL:KRH55745.1};
RA   Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA   Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA   Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA   Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA   Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA   Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA   Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA   Jackson S.;
RT   "WGS assembly of Glycine max.";
RL   Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM000839; KRH55745.1; -; Genomic_DNA.
DR   RefSeq; XP_003527347.1; XM_003527299.3.
DR   AlphaFoldDB; I1KEN4; -.
DR   SMR; I1KEN4; -.
DR   STRING; 3847.I1KEN4; -.
DR   PaxDb; 3847-GLYMA06G43050-1; -.
DR   EnsemblPlants; KRH55745; KRH55745; GLYMA_06G277500.
DR   GeneID; 100807407; -.
DR   Gramene; KRH55745; KRH55745; GLYMA_06G277500.
DR   KEGG; gmx:100807407; -.
DR   eggNOG; ENOG502QPVS; Eukaryota.
DR   HOGENOM; CLU_006557_2_0_1; -.
DR   InParanoid; I1KEN4; -.
DR   OMA; PNSEYDP; -.
DR   OrthoDB; 355614at2759; -.
DR   Proteomes; UP000008827; Chromosome 6.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0048366; P:leaf development; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF47; PHOSPHOENOLPYRUVATE CARBOXYLASE 2; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008827}.
FT   ACT_SITE        173
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        601
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   966 AA;  110276 MW;  D0B1882335CCC80F CRC64;
     MGTRNFEKMA SIDAQLRLLA PSKVSDDDKL VEYDALLLDR FLDILQDLHG DDIRETVQDC
     YELSAEYEGQ NNPQKLEELG NMLTGLDAGD SIVISKSFAH MLNLANLAEE VQIAYRRRIK
     LLKKGDFADE NSAITESDIE ETFKRLVNQL KKTPQEIFDA LKSQTVDLVL TAHPTQSVRR
     SLLQKHGRIR NCLTQLYAKD ITPDDKQELD EALQREIQAA FRTDEIRRTP PTPQDEMRAG
     MSYFHETIWK GIPKFLRRVD TALKNIGINE RVPYNAPVIQ FSSWMGGDRD GNPRVTPEVT
     RDVCLLARMM AANLYFSQIE DLMFELSMWR CNDELRVRSD ELLSSSKRDA KHYIEFWKQI
     PPNEPYRVIL GDVRDKLYNT RERARQLLAN GSSEIPEETT FTNVEQFLEP LELCYRSLCA
     CGDQPIADGS LLDFLRQVST FGLSLVRLDI RQESDRHTDV MDAITNHLEI GSYREWSEER
     RQEWLLSELS GKRPLFGPDL PKTEEIADVL ETFHVIAELP SDSFGAYIIS MATAPSDVLS
     VELLQRECHV KQPLRVVPLF EKLADLEAAP AAVARLFSID WYRDRINGKQ EVMIGYSDSG
     KDAGRFSAAW ALYKAQEELI KVAKEFGVKL TMFHGRGGTV GRGGGPTHLA ILSQPPDTIH
     GSLRVTVQGE VIEQSFGEEH LCFRTLQRFT AATLEHGMHP PVAPKPEWRA LMDEMAVIAT
     EEYRSIVFQE PRFVEYFRCA TPELEYGRMN IGSRPSKRKP SGGIESLRAI PWIFAWTQTR
     FHLPVWLGFG AAFSHVIKKD PKNLQMLQDM YNQWPFFRVS LDLVEMVFAK GDPGIAALYD
     KLLVSEELWP FGERLRSMFE ETKSLLLQVA GHKDLLEGDP YLKQRLRLRD SYITTLNVLQ
     AYTLKRIRDP DYHVKLRPHL SKDYMESNKP AAELVKLNPT SDYAPGLEDT LILTMKGIAA
     GMQNTG
//