I1HPZ8 (I1HPZ8_BRADI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Name:

BRADI2G45420 EnsemblPlants BRADI2G45420.1

Organism

Brachypodium distachyon (Purple false brome) (Trachynia distachya) [Reference proteome] EnsemblPlants BRADI2G45420.1

Taxonomic identifier

15368 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Brachypodieae › Brachypodium

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. RuleBase RU004443 PIRNR PIRNR000108

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 PIRSR PIRSR000108-3 Manganese PIRNR PIRNR000108 PIRSR PIRSR000108-3 Metal-binding PIRNR PIRNR000108 PIRSR PIRSR000108-3 NADP PIRNR PIRNR000108 PIRSR PIRSR000108-4
Molecular function	Oxidoreductase RuleBase RU004444 PIRNR PIRNR000108
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	77 – 79	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	310 – 315	6	NADP By similarity PIRSR PIRSR000108-4
	Region	96 – 102	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Metal binding	252	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Metal binding	275	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Binding site	79	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	84	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	111	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	134	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	260	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	328	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	141	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	212	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	I1HPZ8 [UniParc]. Last modified June 13, 2012. Version 1. Checksum: 3D9AE4FA3E67A005	FASTA	412	46,038
	10 20 30 40 50 60 MAFEKIKVAN PIVEMDGDEM TRVFWQSIKD KLIFPFLDLD IKYYDLGVLH RDATDDKVTV 70 80 90 100 110 120 EAAEATLKYN VAIKCATITP DEDRVKEFNL KQMWRSPNGT IRNIINGTVF REPIICKNVP 130 140 150 160 170 180 KLVPGWTKPI CIGRHAFGDQ YRATDAVLKG PGKLRLVFEG KEETVDLEVF SFTGAGGVAL 190 200 210 220 230 240 SMYNTDESIQ GFAAASMATA YDKKWPLYLS TKNTILKKYD GRFKDIFQEV YEAEWKSKFE 250 260 270 280 290 300 AAGIWYEHRL IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG 310 320 330 340 350 360 KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA RLLEFAQKLE 370 380 390 400 410 DACVGTVESG KMTKDLALLV QGSSNVTRSH YLNTEEFIDA VAAELRTRLA AN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequencing and analysis of the model grass Brachypodium distachyon." International Brachypodium Initiative Nature 463:763-768(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Bd21 EnsemblPlants BRADI2G45420.1.
[2]	EnsemblPlants Submitted (NOV-2012) to UniProtKB Cited for: IDENTIFICATION. Strain: cv. Bd21 EnsemblPlants BRADI2G45420.1.

Cross-references

Sequence databases
RefSeq	XP_003569476.1. XM_003569428.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	BRADI2G45420.1; BRADI2G45420.1; BRADI2G45420.
GeneID	100837485.
KEGG	bdi:100837485.
Phylogenomic databases
KO	K00031.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

I1HPZ8_BRADI

Accession

Primary (citable) accession number: I1HPZ8

Entry history

Integrated into UniProtKB/TrEMBL:	June 13, 2012
Last sequence update:	June 13, 2012
Last modified:	March 6, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)