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Protein

Isocitrate dehydrogenase [NADP]

Gene

BRADI2G45420

Organism
Brachypodium distachyon (Purple false brome) (Trachynia distachya)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei84 – 841NADPUniRule annotation
Binding sitei111 – 1111SubstrateUniRule annotation
Binding sitei134 – 1341SubstrateUniRule annotation
Sitei141 – 1411Critical for catalysisUniRule annotation
Sitei212 – 2121Critical for catalysisUniRule annotation
Metal bindingi252 – 2521Magnesium or manganeseUniRule annotation
Binding sitei260 – 2601NADPUniRule annotation
Metal bindingi275 – 2751Magnesium or manganeseUniRule annotation
Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 793NADPUniRule annotation
Nucleotide bindingi310 – 3156NADPUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Name:BRADI2G45420Imported
OrganismiBrachypodium distachyon (Purple false brome) (Trachynia distachya)Imported
Taxonomic identifieri15368 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeBrachypodieaeBrachypodium
ProteomesiUP000008810: Chromosome 2

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 1027Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

InParanoidiI1HPZ8.
KOiK00031.
OMAiPENGEPQ.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I1HPZ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFEKIKVAN PIVEMDGDEM TRVFWQSIKD KLIFPFLDLD IKYYDLGVLH
60 70 80 90 100
RDATDDKVTV EAAEATLKYN VAIKCATITP DEDRVKEFNL KQMWRSPNGT
110 120 130 140 150
IRNIINGTVF REPIICKNVP KLVPGWTKPI CIGRHAFGDQ YRATDAVLKG
160 170 180 190 200
PGKLRLVFEG KEETVDLEVF SFTGAGGVAL SMYNTDESIQ GFAAASMATA
210 220 230 240 250
YDKKWPLYLS TKNTILKKYD GRFKDIFQEV YEAEWKSKFE AAGIWYEHRL
260 270 280 290 300
IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG
310 320 330 340 350
KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA
360 370 380 390 400
RLLEFAQKLE DACVGTVESG KMTKDLALLV QGSSNVTRSH YLNTEEFIDA
410
VAAELRTRLA AN
Length:412
Mass (Da):46,038
Last modified:June 13, 2012 - v1
Checksum:i3D9AE4FA3E67A005
GO

Sequence databases

RefSeqiXP_003569476.1. XM_003569428.2.

Genome annotation databases

EnsemblPlantsiBRADI2G45420.1; BRADI2G45420.1; BRADI2G45420.
GeneIDi100837485.
KEGGibdi:100837485.

Cross-referencesi

Sequence databases

RefSeqiXP_003569476.1. XM_003569428.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiBRADI2G45420.1; BRADI2G45420.1; BRADI2G45420.
GeneIDi100837485.
KEGGibdi:100837485.

Phylogenomic databases

InParanoidiI1HPZ8.
KOiK00031.
OMAiPENGEPQ.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequencing and analysis of the model grass Brachypodium distachyon."
    International Brachypodium Initiative
    Nature 463:763-768(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Bd21Imported.
  2. EnsemblPlants
    Submitted (NOV-2012) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: cv. Bd21Imported.

Entry informationi

Entry nameiI1HPZ8_BRADI
AccessioniPrimary (citable) accession number: I1HPZ8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: February 4, 2015
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.