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Protein

Alpha-amylase

Gene

BRADI1G35050

Organism
Brachypodium distachyon (Purple false brome) (Trachynia distachya)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei206 – 2061NucleophileUniRule annotation
Active sitei231 – 2311Proton donorUniRule annotation

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Name:BRADI1G35050Imported
OrganismiBrachypodium distachyon (Purple false brome) (Trachynia distachya)Imported
Taxonomic identifieri15368 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeBrachypodieaeBrachypodium
ProteomesiUP000008810 Componenti: Chromosome 1

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Phylogenomic databases

InParanoidiI1GWZ8.
KOiK01176.
OMAiKSDEVGF.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

I1GWZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKHGSHLCC FSLLSLLLLL AGFASGHQVL FQGFNWESWK QQGGWYNMLL
60 70 80 90 100
GKVDDIAAAG VTHVWLPPPS HSVSNEGYMP GRLYDLDASK YGSASELKSL
110 120 130 140 150
IGALHGKGVQ AIADVVINHR CADYKDSRGI YCIFEGGTAD GRLDWGPHMI
160 170 180 190 200
CRDDTAYGDG TANLDTGADF AAAPDIDHLN DRVQRELTDW LLWLKSDLGF
210 220 230 240 250
DAWRLDFAKG YSPEMAKVYI DGTKPGLAVA EVWNDMTPGG DGKPAYDQDA
260 270 280 290 300
HRQALVNWVD RVGGKDSAGM VFDFTTKGIL NVAVEGELWR LIDPQGKAPG
310 320 330 340 350
VVGWWPAKAV TFVDNHDTGS TQAMWPFPAD KVMLGYAYIL THPGTPCIFY
360 370 380 390 400
DHFFNWGFKE EIAALVAVRK RNGITATSAL RILMHDGDAY VAEVDGKVVV
410 420 430 440
KIGSRYDVAA VIPSGFVTTA HGKDYAVWEK AGVAAAAGFQ RSRRSLD
Length:447
Mass (Da):48,750
Last modified:June 13, 2012 - v1
Checksum:i5C1A3A84AFF77F45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU188687 Genomic DNA. No translation available.
RefSeqiXP_003563559.1. XM_003563511.2.

Genome annotation databases

EnsemblPlantsiBRADI1G35050.1; BRADI1G35050.1; BRADI1G35050.
GeneIDi100835083.
KEGGibdi:100835083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU188687 Genomic DNA. No translation available.
RefSeqiXP_003563559.1. XM_003563511.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiBRADI1G35050.1; BRADI1G35050.1; BRADI1G35050.
GeneIDi100835083.
KEGGibdi:100835083.

Phylogenomic databases

InParanoidiI1GWZ8.
KOiK01176.
OMAiKSDEVGF.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequencing and analysis of the model grass Brachypodium distachyon."
    International Brachypodium Initiative
    Nature 463:763-768(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Bd21Imported.
  2. EnsemblPlants
    Submitted (DEC-2013) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: cv. Bd21Imported.

Entry informationi

Entry nameiI1GWZ8_BRADI
AccessioniPrimary (citable) accession number: I1GWZ8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: February 4, 2015
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.