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Protein

Lipoyl synthase, mitochondrial

Gene

COCSUDRAFT_26680

Organism
Coccomyxa subellipsoidea (strain C-169) (Green microalga)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (COCSUDRAFT_48852), Lipoyl synthase, mitochondrial (COCSUDRAFT_26680)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi75Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi79Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi82Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:COCSUDRAFT_26680Imported
OrganismiCoccomyxa subellipsoidea (strain C-169) (Green microalga)Imported
Taxonomic identifieri574566 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeTrebouxiophyceae incertae sedisCoccomyxaceaeCoccomyxa
Proteomesi
  • UP000007264 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI0Z9N9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 274Elp3InterPro annotationAdd BLAST210

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

KOiK03644

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

I0Z9N9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEFLDGAQE KARKPDWLKR TVPGGDKYTE IKAKLRELKL HTVCEEARCP
60 70 80 90 100
NIGECWGGGD GHTATATIML MGDTCTRGCR FCAVKTSKAP PPLDPDEPEN
110 120 130 140 150
TAKAVAAWGI DYVVLTSVDR DDLPDGGAAH ITATIRGLKE KTQGRLLVEA
160 170 180 190 200
LVPDFQGDLD SVSMVAQSGL DVYAHNVETV ERLQGVVRDR RANWAQSLAT
210 220 230 240 250
LRAAKAAGAR ITKTSIMLGC GEQPAEVVDA LQELRDNGVD VVTLGQYMRP
260 270 280 290 300
TKRHMAVAEY VTPEAFAGFE TAAKNMGFMY VAAGPMVRSS YRAGEFYLTN
310
VLREQADAKE AASV
Length:314
Mass (Da):34,051
Last modified:June 13, 2012 - v1
Checksum:i877AEF87E9151997
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AGSI01000001 Genomic DNA Translation: EIE27358.1
RefSeqiXP_005651902.1, XM_005651845.1

Genome annotation databases

GeneIDi17045373
KEGGicsl:COCSUDRAFT_26680

Similar proteinsi

Entry informationi

Entry nameiI0Z9N9_COCSC
AccessioniPrimary (citable) accession number: I0Z9N9
Entry historyiIntegrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: October 25, 2017
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported