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Protein

Kynureninase

Gene

kynU

Organism
Halobacillus halophilus (strain ATCC 35676 / DSM 2266 / JCM 20832 / NBRC 102448/ NCIMB 2269) (Sporosarcina halophila)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.UniRule annotation

Catalytic activityi

L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation
L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathway: L-kynurenine degradation

This protein is involved in step 1 of the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Kynureninase (kynU)
This subpathway is part of the pathway L-kynurenine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.

Pathway: NAD(+) biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Kynureninase (kynU)
  3. no protein annotated in this organism
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021Pyridoxal phosphate; via amide nitrogenUniRule annotation
Binding sitei103 – 1031Pyridoxal phosphateUniRule annotation
Binding sitei211 – 2111Pyridoxal phosphateUniRule annotation
Binding sitei214 – 2141Pyridoxal phosphateUniRule annotation
Binding sitei236 – 2361Pyridoxal phosphateUniRule annotation
Binding sitei265 – 2651Pyridoxal phosphateUniRule annotation
Binding sitei293 – 2931Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Pyridine nucleotide biosynthesisUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

BioCyciHHAL866895:GLDT-3800-MONOMER.
UniPathwayiUPA00253; UER00329.
UPA00334; UER00455.

Names & Taxonomyi

Protein namesi
Recommended name:
KynureninaseUniRule annotation (EC:3.7.1.3UniRule annotation)
Alternative name(s):
L-kynurenine hydrolaseUniRule annotation
Gene namesi
Name:kynUUniRule annotationImported
Ordered Locus Names:HBHAL_4763Imported
OrganismiHalobacillus halophilus (strain ATCC 35676 / DSM 2266 / JCM 20832 / NBRC 102448/ NCIMB 2269) (Sporosarcina halophila)Imported
Taxonomic identifieri866895 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeHalobacillus
ProteomesiUP000007397 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei237 – 2371N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1334Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the kynureninase family.UniRule annotation

Phylogenomic databases

KOiK01556.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.

Sequencei

Sequence statusi: Complete.

I0JSH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPKITLET AAKLDQKDVL HNFKKEFYTD DNRFYMDGNS LGLLSKRAEQ
60 70 80 90 100
SLLSSLEDWK THAIGGWTDG KEPWFYMSEK FGAKTAPLLG AKPEEVISTG
110 120 130 140 150
SITSNLHQLL STFYRPEGQR TKILADELNF PSDIYALKSQ LELHGMDPDE
160 170 180 190 200
HLIQVKSDNG ATLSEEDIIK EMRSDIALLL LPSVLYRSGQ LLDIEKITKA
210 220 230 240 250
AHEQGIMVGF DLAHSIGALP HNLDDWGVDF AVWCTYKYLN SGPGGVGGLY
260 270 280 290 300
VNEKHLGSKP GLAGWFSSKK DKQFDMAHDL NHAETAGAYQ MGTPHILSSA
310 320 330 340 350
PLLGSLDLFQ EADIKNVRCK SLQMTRLMLD LVYQELDGLG FQIITPLEDE
360 370 380 390 400
RRGGHISLVH SEAASICKAL KKENVVPDFR APDVIRLAPV ALYTTYKEVY
410 420
EVMMILKEIM VNETYKKYKN ERDIIA
Length:426
Mass (Da):47,758
Last modified:June 13, 2012 - v1
Checksum:i3281C3699AF84B54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE717023 Genomic DNA. Translation: CCG47101.1.
RefSeqiWP_014644985.1. NC_017668.1.
YP_006182372.1. NC_017668.1.

Genome annotation databases

EnsemblBacteriaiCCG47101; CCG47101; HBHAL_4763.
KEGGihhd:HBHAL_4763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE717023 Genomic DNA. Translation: CCG47101.1.
RefSeqiWP_014644985.1. NC_017668.1.
YP_006182372.1. NC_017668.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCG47101; CCG47101; HBHAL_4763.
KEGGihhd:HBHAL_4763.

Phylogenomic databases

KOiK01556.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
UPA00334; UER00455.
BioCyciHHAL866895:GLDT-3800-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Chloride and organic osmolytes: a hybrid strategy to cope with elevated salinities by the moderately halophilic, chloride-dependent bacterium Halobacillus halophilus."
    Saum S.H., Pfeiffer F., Palm P., Rampp M., Schuster S.C., Muller V., Oesterhelt D.
    Environ. Microbiol. 15:1619-1633(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35676 / DSM 2266 / JCM 20832 / NBRC 102448/ NCIMB 2269Imported.

Entry informationi

Entry nameiI0JSH9_HALH3
AccessioniPrimary (citable) accession number: I0JSH9
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: May 27, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.