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I0JF26 (I0JF26_STAAU) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:SAEMRSA15_19920 EMBL CCG16703.1
OrganismStaphylococcus aureus subsp. aureus HO 5096 0412 [Complete proteome] EMBL CCG16703.1
Taxonomic identifier1074252 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 339206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding167 – 22256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2931Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I0JF26 [UniParc].

Last modified June 13, 2012. Version 1.
Checksum: 658118B0958DC645

FASTA35640,221
        10         20         30         40         50         60 
MTKENICIVF GGKSAEHEVS ILTAQNVLNA IDKDKYHVDI IYITNDGDWR KQNNITAEIK 

        70         80         90        100        110        120 
STDELHLENG EALEISQLLK ESSSGQPYDA VFPLLHGPNG EDGTIQGLFE VLDVPYVGNG 

       130        140        150        160        170        180 
VLSAASSMDK LVMKQLFEHR GLPQLPYISF LRSEYEKYEH NILKLVNDKL NYPVFVKPAN 

       190        200        210        220        230        240 
LGSSVGISKC NNEAELKEGI KEAFQFDRKL VIEQGVNARE IEVAVLGNDY PEATWPGEVV 

       250        260        270        280        290        300 
KDVAFYDYKS KYKDGKVQLQ IPADLDEDVQ LTLRNMALEA FKATDCSGLV RADFFVTEDN 

       310        320        330        340        350 
QIYINETNAM PGFTAFSMYP KLWENMGLSY SELITKLIEL AKERHQDKQK NKYKID 

« Hide

References

[1]Aslett M.
Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: HO 5096 0412 EMBL CCG16703.1.
[2]"A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic."
Holden M.T., Hsu L.Y., Kurt K., Weinert L.A., Mather A.E., Harris S.R., Strommenger B., Layer F., Witte W., de Lencastre H., Skov R., Westh H., Zemlickova H., Coombs G., Kearns A.M., Hill R.L., Edgeworth J., Gould I. expand/collapse author list , Gant V., Cooke J., Edwards G.F., McAdam P.R., Templeton K.E., McCann A., Zhou Z., Castillo-Ramirez S., Feil E.J., Hudson L.O., Enright M.C., Balloux F., Aanensen D.M., Spratt B.G., Fitzgerald J.R., Parkhill J., Achtman M., Bentley S.D., Nubel U.
Genome Res. 23:653-664(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: HO 5096 0412 EMBL CCG16703.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HE681097 Genomic DNA. Translation: CCG16703.1.
RefSeqYP_006238320.1. NC_017763.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCG16703; CCG16703; SAEMRSA15_19920.
GeneID12801107.
KEGGsux:SAEMRSA15_19920.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycSAUR1074252:GLKL-2079-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI0JF26_STAAU
AccessionPrimary (citable) accession number: I0JF26
Entry history
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: February 19, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)