Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-enolase

Gene

ENO1

Organism
Thunnus albacares (Yellowfin tuna) (Neothunnus macropterus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses.By similarity

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Cofactori

Mg2+By similarityNote: Binds two Mg2+ per subunit. Required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Alpha-enolase (ENO1), Beta-enolase (ENO3)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Magnesium 1By similarity1
Binding sitei158SubstrateBy similarity1
Binding sitei167SubstrateBy similarity1
Active sitei210Proton donorBy similarity1
Metal bindingi245Magnesium 2By similarity1
Metal bindingi293Magnesium 2By similarity1
Binding sitei293SubstrateBy similarity1
Metal bindingi318Magnesium 2By similarity1
Binding sitei318SubstrateBy similarity1
Active sitei343Proton acceptorBy similarity1
Binding sitei394SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-enolaseBy similarity (EC:4.2.1.11By similarity)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseBy similarity
Enolase 1By similarity
Allergen: Thu a 2.01011 Publication
Gene namesi
Name:ENO1By similarity
OrganismiThunnus albacares (Yellowfin tuna) (Neothunnus macropterus)
Taxonomic identifieri8236 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataPelagiariaScombriformesScombridaeThunnus

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei10132. Thu a 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004250782 – 432Alpha-enolaseBy similarityAdd BLAST431

Proteomic databases

PRIDEiI0J1J1.

Interactioni

Subunit structurei

Dimer.By similarity

Structurei

3D structure databases

SMRiI0J1J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni370 – 373Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Sequence analysis

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

I0J1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILKIHARE IFDSRGNPTV EVDLYTKKGL FRAAVPSGAS TGIYEALELR
60 70 80 90 100
DNDKTRYMGK GVKRAVKYIN EFLAPALCNQ DVTVIEQEKI DKLMLDMDGT
110 120 130 140 150
ENKYKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PEVILPVPAF
160 170 180 190 200
NVINGGSHAG NKLAMQEFMI LPVGASSFKD AMRIGAEVYH NLKNVIKEKY
210 220 230 240 250
GKDATNVGDE GGFAPNILEN KEALKLLKNA IGKAGYTDKI VIGMDVAASE
260 270 280 290 300
FYKGGKYDLD FKSPDDPSRY IPSDKLADLY KGFVKDYPVV SIEDPFDQDD
310 320 330 340 350
WEAWSKFTAS TSIQVVGDDL TVTNPKRIAK GVAEKSCNCL LLKVNQIGSV
360 370 380 390 400
TESLQACKMA QSSGWGVMVS HRSGETEDTL ISDLVVGLCT GQIKTGAPCR
410 420 430
SERLAKYNQL MRIEEELGAK AKFAGKNFRH PI
Length:432
Mass (Da):47,136
Last modified:June 13, 2012 - v1
Checksum:i331D80F26AA76D82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN812740 mRNA. Translation: CBL79145.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENOA_THUAL
AccessioniPrimary (citable) accession number: I0J1J1
Secondary accession number(s): B3A0L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: June 13, 2012
Last modified: April 12, 2017
This is version 17 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families