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Protein

Alpha-amylase

Gene

AMIS_23270

Organism
Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Ordered Locus Names:AMIS_23270Imported
OrganismiActinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)Imported
Taxonomic identifieri512565 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeActinoplanes
Proteomesi
  • UP000007882 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_500362761738 – 588Alpha-amylaseSequence analysisAdd BLAST551

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini487 – 588CBM20 (carbohydrate binding type-20)InterPro annotationAdd BLAST102

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotationSAAS annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

KOiK01176.
OMAiGQNIFVV.
OrthoDBiPOG091H0CDS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF00686. CBM_20. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I0H3G0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRRRSRLTT ALAATTLAVG LGGFGLSSII TGPDALAAPP GSKDVTAVMF
60 70 80 90 100
EWSFASVARE CTNRLGPLGY GYVQVSPPQE HIQGSQWWTS YQPVSYRIAG
110 120 130 140 150
RLGDRNAFAS MVSTCHAAGV KVVADTVINH MSAGSGTGTG GTGYSKYNYP
160 170 180 190 200
GYYSDADFHS CRTAISDYTN RSNVQDCELV NLSDLNTGSD YVRGKIAGYM
210 220 230 240 250
NDLASLGVDG FRIDAAKHMS ASDLSGIKAK LSNTGAYWKQ EVIFGAGEAV
260 270 280 290 300
QPTEYTGTGD VQEFRYARDL KRIFLSENLA YLSNFGASWG YLPSNQAAVF
310 320 330 340 350
VDNHDTERGG DTLSYKNGSA YTLAQVFALA WPYGAPDVNS GYEFSDHDAG
360 370 380 390 400
PPNGGTVNAC YSDGWKCQHA WRQIGNMVGF RNGVSGTAVT NWWSNGGDQI
410 420 430 440 450
AFGRGSKGYV AINHEGNSLT RTFQTSLPAG TYCDVQHGDP VAGGGCTGTT
460 470 480 490 500
YTVNTSGQFT ATIPAGEAVA LYVGATGGVT PTTGTTTNPP AGSGASFGVT
510 520 530 540 550
ATTTLGQNIF VVGNQAALGN WAPASAVALS SASYPVWKGT VTLPAGTSFE
560 570 580
YKYLRKNADG SVTWESGGNR TATVPASGSV TLSDTWRN
Length:588
Mass (Da):61,804
Last modified:June 13, 2012 - v1
Checksum:i55286CF040819F6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012319 Genomic DNA. Translation: BAL87547.1.

Genome annotation databases

EnsemblBacteriaiBAL87547; BAL87547; AMIS_23270.
KEGGiams:AMIS_23270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012319 Genomic DNA. Translation: BAL87547.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAL87547; BAL87547; AMIS_23270.
KEGGiams:AMIS_23270.

Phylogenomic databases

KOiK01176.
OMAiGQNIFVV.
OrthoDBiPOG091H0CDS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF00686. CBM_20. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiI0H3G0_ACTM4
AccessioniPrimary (citable) accession number: I0H3G0
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: November 30, 2016
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.