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I0GYV7

- I0GYV7_ACTM4

UniProt

I0GYV7 - I0GYV7_ACTM4

Protein

Isocitrate dehydrogenase [NADP]

Gene

AMIS_7240

Organism
Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (13 Jun 2012)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateUniRule annotation
    Binding sitei82 – 821NADP
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Sitei139 – 1391Critical for catalysisUniRule annotation
    Sitei211 – 2111Critical for catalysisUniRule annotation
    Metal bindingi251 – 2511Magnesium or manganeseUniRule annotation
    Binding sitei259 – 2591NADP
    Metal bindingi274 – 2741Magnesium or manganeseUniRule annotation
    Binding sitei327 – 3271NADP; via amide nitrogen and carbonyl oxygen

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADP
    Nucleotide bindingi309 – 3146NADP

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Enzyme and pathway databases

    BioCyciAMIS512565:GL7J-744-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    Ordered Locus Names:AMIS_7240Imported
    OrganismiActinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)Imported
    Taxonomic identifieri512565 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes
    ProteomesiUP000007882: Chromosome

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    KOiK00031.
    OMAiFKDEFQR.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    I0GYV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKIKVKNPV VEIDGDEMTR IIWKQIREQL VLPYLDVNLE YYDLSIQYRD    50
    ETDDQVTIDA ANAIKQHGVG VKCATITPDE ARVEEFGLKK MWRSPNGTIR 100
    NILGGVVFRE PIIMSNVPRL VPSWTKPIII GRHAHGDQYK ATDFVAPSAG 150
    KMTVTFTPAD GSAPMEFVVS DFPAGGVGMA MYNYDESIRD FARASFRYGL 200
    ARNYPVYLST KNTILKAYDG RFKDLFAEIF ETEFADQFKA AGLTYEHRLI 250
    DDMVAAALKW EGGYVWACKN YDGDVQSDTV AQGFGSLGLM TSVLMTPDGK 300
    TVEAEAAHGT VTRHYRQWQK GEKTSTNPIA SIFAWTGGLK HRGKLDGAPE 350
    VTQFAETLER VCIETVEGGQ MTKDLALLIA KDAPWLSTDE FMNALDENLA 400
    RKLAA 405
    Length:405
    Mass (Da):45,079
    Last modified:June 13, 2012 - v1
    Checksum:iFE1D1A3F018B17AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP012319 Genomic DNA. Translation: BAL85944.1.
    RefSeqiWP_014440841.1. NC_017093.1.
    YP_005460460.1. NC_017093.1.

    Genome annotation databases

    EnsemblBacteriaiBAL85944; BAL85944; AMIS_7240.
    GeneIDi12049651.
    KEGGiams:AMIS_7240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP012319 Genomic DNA. Translation: BAL85944.1 .
    RefSeqi WP_014440841.1. NC_017093.1.
    YP_005460460.1. NC_017093.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAL85944 ; BAL85944 ; AMIS_7240 .
    GeneIDi 12049651.
    KEGGi ams:AMIS_7240.

    Phylogenomic databases

    KOi K00031.
    OMAi FKDEFQR.

    Enzyme and pathway databases

    BioCyci AMIS512565:GL7J-744-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Actinoplanes missouriensis 431 (= NBRC 102363)."
      Ohnishi Y., Ishikawa J., Sekine M., Hosoyama A., Harada T., Narita H., Hata T., Konno Y., Tutikane K., Fujita N., Horinouchi S., Hayakawa M.
      Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431Imported.

    Entry informationi

    Entry nameiI0GYV7_ACTM4
    AccessioniPrimary (citable) accession number: I0GYV7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 13, 2012
    Last sequence update: June 13, 2012
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3