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Protein

Isocitrate dehydrogenase [NADP]

Gene

AMIS_7240

Organism
Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Binding sitei82NADPUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Sitei139Critical for catalysisUniRule annotation1
Sitei211Critical for catalysisUniRule annotation1
Metal bindingi251Magnesium or manganeseUniRule annotation1
Binding sitei259NADPUniRule annotation1
Metal bindingi274Magnesium or manganeseUniRule annotation1
Binding sitei327NADP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPUniRule annotation3
Nucleotide bindingi309 – 314NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:AMIS_7240Imported
OrganismiActinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)Imported
Taxonomic identifieri512565 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeActinoplanes
Proteomesi
  • UP000007882 Componenti: Chromosome

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 395Iso_dhInterPro annotationAdd BLAST387

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

KOiK00031.
OMAiTVQRHYY.
OrthoDBiPOG091H0JP0.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I0GYV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIKVKNPV VEIDGDEMTR IIWKQIREQL VLPYLDVNLE YYDLSIQYRD
60 70 80 90 100
ETDDQVTIDA ANAIKQHGVG VKCATITPDE ARVEEFGLKK MWRSPNGTIR
110 120 130 140 150
NILGGVVFRE PIIMSNVPRL VPSWTKPIII GRHAHGDQYK ATDFVAPSAG
160 170 180 190 200
KMTVTFTPAD GSAPMEFVVS DFPAGGVGMA MYNYDESIRD FARASFRYGL
210 220 230 240 250
ARNYPVYLST KNTILKAYDG RFKDLFAEIF ETEFADQFKA AGLTYEHRLI
260 270 280 290 300
DDMVAAALKW EGGYVWACKN YDGDVQSDTV AQGFGSLGLM TSVLMTPDGK
310 320 330 340 350
TVEAEAAHGT VTRHYRQWQK GEKTSTNPIA SIFAWTGGLK HRGKLDGAPE
360 370 380 390 400
VTQFAETLER VCIETVEGGQ MTKDLALLIA KDAPWLSTDE FMNALDENLA

RKLAA
Length:405
Mass (Da):45,079
Last modified:June 13, 2012 - v1
Checksum:iFE1D1A3F018B17AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012319 Genomic DNA. Translation: BAL85944.1.
RefSeqiWP_014440841.1. NC_017093.1.

Genome annotation databases

EnsemblBacteriaiBAL85944; BAL85944; AMIS_7240.
KEGGiams:AMIS_7240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012319 Genomic DNA. Translation: BAL85944.1.
RefSeqiWP_014440841.1. NC_017093.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAL85944; BAL85944; AMIS_7240.
KEGGiams:AMIS_7240.

Phylogenomic databases

KOiK00031.
OMAiTVQRHYY.
OrthoDBiPOG091H0JP0.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiI0GYV7_ACTM4
AccessioniPrimary (citable) accession number: I0GYV7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: November 2, 2016
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.