Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Selenomonas ruminantium subsp. lactilytica (strain NBRC 103574 / TAM6421)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151Diphosphate; via amide nitrogenUniRule annotation
Metal bindingi109 – 1091Magnesium; catalyticUniRule annotation
Sitei110 – 1101Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
Sitei136 – 1361Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei139 – 1391Proton acceptorUniRule annotation
Binding sitei245 – 2451SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciSRUM927704:GLJZ-706-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:SELR_06670Imported
OrganismiSelenomonas ruminantium subsp. lactilytica (strain NBRC 103574 / TAM6421)Imported
Taxonomic identifieri927704 [NCBI]
Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesVeillonellaceaeSelenomonas
ProteomesiUP000007887 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1393Substrate bindingUniRule annotation
Regioni184 – 1863Substrate bindingUniRule annotation
Regioni305 – 3084Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "Short" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_01979. Phosphofructokinase_II_Short.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011403. PPi-PFK_TM0289.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036482. PPi_PFK_TM0289. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

I0GNN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGIKNVIAV VCGGGPAPGI NSVISGVVNE ATRQGWDVLG MYDGFSRLAR
60 70 80 90 100
GEKNYIRLEP KNISRIHLTG GCILKMSRFN PTKKESDLRT VVETLTELGV
110 120 130 140 150
THLVTIGGDD TAYSSAAVSD YARKMGRTIN VVHVPKTIDN DLPLPEGIPT
160 170 180 190 200
FGFETARAFG TQEVENLMED ARTTNNRWYF TIAMGRTAGH LALGMGRSAG
210 220 230 240 250
AALTIIPEEF PEDKIPLQQV VDIITGAIVK RYLTGKNYGV AVIAEGVIEK
260 270 280 290 300
IAPEDFKKLG TVVTDEHGHI RYSELDFGEI LKQAVLAEVK KLGIKITVID
310 320 330 340 350
KEIGYELRCT APIAYDIDYA RQLGYSAVQF LMSGDSGALI SIQDNKAVPM
360 370 380 390 400
RFEDIKDPAT GKTMVRKVNI DSVHYQIARG LMMRLEKGDL DDPGLANAYR
410 420
MEPEEFKQRY SYLFPEEPDV TAEA
Length:424
Mass (Da):46,609
Last modified:June 13, 2012 - v1
Checksum:i0112F90CF0EAB574
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012292 Genomic DNA. Translation: BAL82375.1.
RefSeqiWP_014423816.1. NC_017068.1.
YP_005432398.1. NC_017068.1.

Genome annotation databases

EnsemblBacteriaiBAL82375; BAL82375; SELR_06670.
KEGGisri:SELR_06670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012292 Genomic DNA. Translation: BAL82375.1.
RefSeqiWP_014423816.1. NC_017068.1.
YP_005432398.1. NC_017068.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAL82375; BAL82375; SELR_06670.
KEGGisri:SELR_06670.

Phylogenomic databases

KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciSRUM927704:GLJZ-706-MONOMER.

Family and domain databases

HAMAPiMF_01979. Phosphofructokinase_II_Short.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011403. PPi-PFK_TM0289.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036482. PPi_PFK_TM0289. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Whole genome sequence of Selenomonas ruminantium subsp. lactilytica TAM6421."
    Oguchi A., Ankai A., Kaneko J., Yamada-Narita S., Fukui S., Takahashi M., Onodera T., Kojima S., Fushimi T., Abe N., Kamio Y., Yamazaki S., Fujita N.
    Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 103574 / TAM6421Imported.

Entry informationi

Entry nameiI0GNN8_SELRL
AccessioniPrimary (citable) accession number: I0GNN8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: May 27, 2015
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.