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I0EWI2 (I0EWI2_HELPX) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFI07301.1
ORF Names:HPB14_02970 EMBL AFI07301.1
OrganismHelicobacter pylori HUP-B14 [Complete proteome] EMBL AFI07301.1
Taxonomic identifier1163743 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 332199ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding161 – 21656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2881Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3001Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I0EWI2 [UniParc].

Last modified June 13, 2012. Version 1.
Checksum: 132247421B9F2C04

FASTA34739,720
        10         20         30         40         50         60 
MEFCVLFGGA SFEHEISIVS AIALKGVLKD RIKYFIFLDE NHHFYLIEES NMHSKYFAQI 

        70         80         90        100        110        120 
KEKKLSPLIL THNGLLKNSF LGTKIIELPL VINLVHGGDG EDGKLASLLE FYRIAFIGPR 

       130        140        150        160        170        180 
IEASVLSYNK YLTKLYAKDL GVKTLDYVLL NEKNRANALD LIGFNFPFIV KPSNAGSSLG 

       190        200        210        220        230        240 
VSVVKEEKEL IYALDSAFEY SKEVLIEPFI QGVKEYNLAG CKIKKDFCFS YVEEPNKQEF 

       250        260        270        280        290        300 
LDFKQKYLDF SRNKAPKANL SNALEEQLKE NFKKLYNDLF DGAIIRCDFF VIENEVYLNE 

       310        320        330        340 
INPIPGSLAN YLFDDFKITL ENLVQSLPKP PKIQIKNSYL LQIQKNK 

« Hide

References

[1]Kersulyte D., Berg D.E.
Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: HUP-B14 EMBL AFI07301.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003486 Genomic DNA. Translation: AFI07301.1.
RefSeqYP_006219321.1. NC_017733.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFI07301; AFI07301; HPB14_02970.
GeneID12780394.
KEGGhcn:HPB14_02970.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycHPYL1163743:GLEH-592-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI0EWI2_HELPX
AccessionPrimary (citable) accession number: I0EWI2
Entry history
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: June 11, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)