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I0DUN8

- I0DUN8_PROSM

UniProt

I0DUN8 - I0DUN8_PROSM

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Providencia stuartii (strain MRSN 2154)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (13 Jun 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
    Active sitei131 – 1311Proton acceptorUniRule annotation
    Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
    Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei217 – 2171Allosteric activator ADPUniRule annotation
    Binding sitei228 – 2281SubstrateUniRule annotation
    Binding sitei249 – 2491Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772ATPUniRule annotation
    Nucleotide bindingi106 – 1094ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPSTU1157951:GLIN-2190-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:S70_11025Imported
    OrganismiProvidencia stuartii (strain MRSN 2154)Imported
    Taxonomic identifieri1157951 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia
    ProteomesiUP000005012: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliI0DUN8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni58 – 636Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni129 – 1313Substrate bindingUniRule annotation
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
    Regioni219 – 2213Allosteric activator ADP bindingUniRule annotation
    Regioni255 – 2584Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00850.
    OMAiGFGGRCV.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    I0DUN8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKQIKRIGV LTSGGDAPGM NAAIRGVVRA ALSEGLEVMG IYDGYLGLYE    50
    NRMKQLDRYS VSDMINRGGT FLGSARFPQF RDEKVRAVAI DNLKKNHIDA 100
    LVVIGGDGSY LGAKSLTEAG FPCIGLPGTI DNDVAGTDYT IGYFTALETV 150
    VEAIDRLRDT STSHKRISIV EVMGRYCGDL TLSAAIAGGC EFIVLPENEM 200
    SFDREELLSE IKQGIEKGKR HAIVAITEHV CDVDELAKYI EAETHHETRA 250
    TVLGHIQRGG SPVAYDRILA SRMGAYSIQL LLEGYGGRCV GIQNEKLVHH 300
    DIIDAVMNMK RVFKKEWYDT AKKLY 325
    Length:325
    Mass (Da):35,724
    Last modified:June 13, 2012 - v1
    Checksum:iCB817C72FA41D809
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003488 Genomic DNA. Translation: AFH94056.1.
    RefSeqiYP_006216747.1. NC_017731.1.

    Genome annotation databases

    EnsemblBacteriaiAFH94056; AFH94056; S70_11025.
    GeneIDi12785026.
    KEGGipsi:S70_11025.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003488 Genomic DNA. Translation: AFH94056.1 .
    RefSeqi YP_006216747.1. NC_017731.1.

    3D structure databases

    ProteinModelPortali I0DUN8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFH94056 ; AFH94056 ; S70_11025 .
    GeneIDi 12785026.
    KEGGi psi:S70_11025.

    Phylogenomic databases

    KOi K00850.
    OMAi GFGGRCV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci PSTU1157951:GLIN-2190-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Providencia stuartii clinical isolate MRSN 2154."
      Clifford R.J., Hang J., Riley M.C., Onmus-Leone F., Kuschner R.A., Lesho E.P., Waterman P.E.
      Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MRSN 2154Imported.

    Entry informationi

    Entry nameiI0DUN8_PROSM
    AccessioniPrimary (citable) accession number: I0DUN8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 13, 2012
    Last sequence update: June 13, 2012
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3