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I0DUN8 (I0DUN8_PROSM) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339

Short name=ATP-PFK HAMAP-Rule MF_00339
Short name=Phosphofructokinase HAMAP-Rule MF_00339
EC=2.7.1.11 HAMAP-Rule MF_00339
Alternative name(s):
Phosphohexokinase HAMAP-Rule MF_00339
Gene names
Name:pfkA HAMAP-Rule MF_00339
Ordered Locus Names:S70_11025 EMBL AFH94056.1
OrganismProvidencia stuartii (strain MRSN 2154) [Complete proteome] [HAMAP] EMBL AFH94056.1
Taxonomic identifier1157951 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339 SAAS SAAS022953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339 SAAS SAAS012828

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339 SAAS SAAS022953.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily. HAMAP-Rule MF_00339

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding76 – 772ATP By similarity HAMAP-Rule MF_00339
Nucleotide binding106 – 1094ATP By similarity HAMAP-Rule MF_00339
Region25 – 295Allosteric activator ADP binding; shared with dimeric partner By similarity HAMAP-Rule MF_00339
Region58 – 636Allosteric activator ADP binding; shared with dimeric partner By similarity HAMAP-Rule MF_00339
Region129 – 1313Substrate binding By similarity HAMAP-Rule MF_00339
Region173 – 1753Substrate binding By similarity HAMAP-Rule MF_00339
Region189 – 1913Allosteric activator ADP binding By similarity HAMAP-Rule MF_00339
Region219 – 2213Allosteric activator ADP binding By similarity HAMAP-Rule MF_00339
Region255 – 2584Substrate binding By similarity HAMAP-Rule MF_00339

Sites

Active site1311Proton acceptor By similarity HAMAP-Rule MF_00339
Metal binding1071Magnesium; catalytic By similarity HAMAP-Rule MF_00339
Binding site151ATP; via amide nitrogen By similarity HAMAP-Rule MF_00339
Binding site1581Allosteric activator ADP By similarity HAMAP-Rule MF_00339
Binding site1661Substrate; shared with dimeric partner By similarity HAMAP-Rule MF_00339
Binding site2171Allosteric activator ADP By similarity HAMAP-Rule MF_00339
Binding site2281Substrate By similarity HAMAP-Rule MF_00339
Binding site2491Substrate; shared with dimeric partner By similarity HAMAP-Rule MF_00339

Sequences

Sequence LengthMass (Da)Tools
I0DUN8 [UniParc].

Last modified June 13, 2012. Version 1.
Checksum: CB817C72FA41D809

FASTA32535,724
        10         20         30         40         50         60 
MVKQIKRIGV LTSGGDAPGM NAAIRGVVRA ALSEGLEVMG IYDGYLGLYE NRMKQLDRYS 

        70         80         90        100        110        120 
VSDMINRGGT FLGSARFPQF RDEKVRAVAI DNLKKNHIDA LVVIGGDGSY LGAKSLTEAG 

       130        140        150        160        170        180 
FPCIGLPGTI DNDVAGTDYT IGYFTALETV VEAIDRLRDT STSHKRISIV EVMGRYCGDL 

       190        200        210        220        230        240 
TLSAAIAGGC EFIVLPENEM SFDREELLSE IKQGIEKGKR HAIVAITEHV CDVDELAKYI 

       250        260        270        280        290        300 
EAETHHETRA TVLGHIQRGG SPVAYDRILA SRMGAYSIQL LLEGYGGRCV GIQNEKLVHH 

       310        320 
DIIDAVMNMK RVFKKEWYDT AKKLY 

« Hide

References

[1]"Complete genome sequence of Providencia stuartii clinical isolate MRSN 2154."
Clifford R.J., Hang J., Riley M.C., Onmus-Leone F., Kuschner R.A., Lesho E.P., Waterman P.E.
Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MRSN 2154.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003488 Genomic DNA. Translation: AFH94056.1.
RefSeqYP_006216747.1. NC_017731.1.

3D structure databases

ProteinModelPortalI0DUN8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFH94056; AFH94056; S70_11025.
GeneID12785026.
KEGGpsi:S70_11025.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00850.
OMAGFGGRCV.

Enzyme and pathway databases

BioCycPSTU1157951:GLIN-2190-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI0DUN8_PROSM
AccessionPrimary (citable) accession number: I0DUN8
Entry history
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: July 9, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)