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I0DUN8

- I0DUN8_PROSM

UniProt

I0DUN8 - I0DUN8_PROSM

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Providencia stuartii (strain MRSN 2154)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Note: Magnesium.UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
Active sitei131 – 1311Proton acceptorUniRule annotation
Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
Binding sitei217 – 2171Allosteric activator ADPUniRule annotation
Binding sitei228 – 2281SubstrateUniRule annotation
Binding sitei249 – 2491Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi106 – 1094ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSTU1157951:GLIN-2190-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:S70_11025Imported
OrganismiProvidencia stuartii (strain MRSN 2154)Imported
Taxonomic identifieri1157951 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia
ProteomesiUP000005012: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliI0DUN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni58 – 636Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni129 – 1313Substrate bindingUniRule annotation
Regioni173 – 1753Substrate bindingUniRule annotation
Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
Regioni219 – 2213Allosteric activator ADP bindingUniRule annotation
Regioni255 – 2584Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiGFGGRCV.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I0DUN8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKQIKRIGV LTSGGDAPGM NAAIRGVVRA ALSEGLEVMG IYDGYLGLYE
60 70 80 90 100
NRMKQLDRYS VSDMINRGGT FLGSARFPQF RDEKVRAVAI DNLKKNHIDA
110 120 130 140 150
LVVIGGDGSY LGAKSLTEAG FPCIGLPGTI DNDVAGTDYT IGYFTALETV
160 170 180 190 200
VEAIDRLRDT STSHKRISIV EVMGRYCGDL TLSAAIAGGC EFIVLPENEM
210 220 230 240 250
SFDREELLSE IKQGIEKGKR HAIVAITEHV CDVDELAKYI EAETHHETRA
260 270 280 290 300
TVLGHIQRGG SPVAYDRILA SRMGAYSIQL LLEGYGGRCV GIQNEKLVHH
310 320
DIIDAVMNMK RVFKKEWYDT AKKLY
Length:325
Mass (Da):35,724
Last modified:June 13, 2012 - v1
Checksum:iCB817C72FA41D809
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003488 Genomic DNA. Translation: AFH94056.1.
RefSeqiWP_004924408.1. NC_017731.1.
YP_006216747.1. NC_017731.1.

Genome annotation databases

EnsemblBacteriaiAFH94056; AFH94056; S70_11025.
GeneIDi12785026.
KEGGipsi:S70_11025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003488 Genomic DNA. Translation: AFH94056.1 .
RefSeqi WP_004924408.1. NC_017731.1.
YP_006216747.1. NC_017731.1.

3D structure databases

ProteinModelPortali I0DUN8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFH94056 ; AFH94056 ; S70_11025 .
GeneIDi 12785026.
KEGGi psi:S70_11025.

Phylogenomic databases

KOi K00850.
OMAi GFGGRCV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci PSTU1157951:GLIN-2190-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Providencia stuartii clinical isolate MRSN 2154."
    Clifford R.J., Hang J., Riley M.C., Onmus-Leone F., Kuschner R.A., Lesho E.P., Waterman P.E.
    Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSN 2154Imported.

Entry informationi

Entry nameiI0DUN8_PROSM
AccessioniPrimary (citable) accession number: I0DUN8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: November 26, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3