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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Providencia stuartii (strain MRSN 2154)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
Active sitei131 – 1311Proton acceptorUniRule annotation
Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
Binding sitei217 – 2171Allosteric activator ADPUniRule annotation
Binding sitei228 – 2281SubstrateUniRule annotation
Binding sitei249 – 2491Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi106 – 1094ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSTU1157951:GLIN-2190-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:S70_11025Imported
OrganismiProvidencia stuartii (strain MRSN 2154)Imported
Taxonomic identifieri1157951 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia
ProteomesiUP000005012 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliI0DUN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni58 – 636Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni129 – 1313Substrate bindingUniRule annotation
Regioni173 – 1753Substrate bindingUniRule annotation
Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
Regioni219 – 2213Allosteric activator ADP bindingUniRule annotation
Regioni255 – 2584Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiVHHDIVD.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I0DUN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKQIKRIGV LTSGGDAPGM NAAIRGVVRA ALSEGLEVMG IYDGYLGLYE
60 70 80 90 100
NRMKQLDRYS VSDMINRGGT FLGSARFPQF RDEKVRAVAI DNLKKNHIDA
110 120 130 140 150
LVVIGGDGSY LGAKSLTEAG FPCIGLPGTI DNDVAGTDYT IGYFTALETV
160 170 180 190 200
VEAIDRLRDT STSHKRISIV EVMGRYCGDL TLSAAIAGGC EFIVLPENEM
210 220 230 240 250
SFDREELLSE IKQGIEKGKR HAIVAITEHV CDVDELAKYI EAETHHETRA
260 270 280 290 300
TVLGHIQRGG SPVAYDRILA SRMGAYSIQL LLEGYGGRCV GIQNEKLVHH
310 320
DIIDAVMNMK RVFKKEWYDT AKKLY
Length:325
Mass (Da):35,724
Last modified:June 13, 2012 - v1
Checksum:iCB817C72FA41D809
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003488 Genomic DNA. Translation: AFH94056.1.
RefSeqiWP_004924408.1. NC_017731.1.
YP_006216747.1. NC_017731.1.

Genome annotation databases

EnsemblBacteriaiAFH94056; AFH94056; S70_11025.
KEGGipsi:S70_11025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003488 Genomic DNA. Translation: AFH94056.1.
RefSeqiWP_004924408.1. NC_017731.1.
YP_006216747.1. NC_017731.1.

3D structure databases

ProteinModelPortaliI0DUN8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFH94056; AFH94056; S70_11025.
KEGGipsi:S70_11025.

Phylogenomic databases

KOiK00850.
OMAiVHHDIVD.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciPSTU1157951:GLIN-2190-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Providencia stuartii clinical isolate MRSN 2154."
    Clifford R.J., Hang J., Riley M.C., Onmus-Leone F., Kuschner R.A., Lesho E.P., Waterman P.E.
    Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSN 2154Imported.

Entry informationi

Entry nameiI0DUN8_PROSM
AccessioniPrimary (citable) accession number: I0DUN8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: June 24, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.