I0CME7 (HYAL_CONCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hyaluronidase conohyal-Cn1 Short name=Hya EC=3.2.1.35 Alternative name(s): Hyaluronoglucosaminidase
Organism	Conus consors (Singed cone)
Taxonomic identifier	101297 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hyaluronidase catalyzes the hydrolysis of hyaluronic acid (HA), an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. In venom, they are known to enhance diffusion of the venom by degrading the extracellular matrix.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Miscellaneous	Found in both injectable (milked) (IV) and dissected venom (DV) (Ref.1).
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 33	15			PRO_0000419902
	Chain	34 – 448	415	Hyaluronidase conohyal-Cn1		PRO_0000419903
Regions
	Domain	413 – 424	12	EGF-like
Sites
	Active site	151	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	169	1	N-linked (GlcNAc...) Probable
	Glycosylation	361	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	67 ↔ 344		By similarity
	Disulfide bond	369 ↔ 380		By similarity
	Disulfide bond	374 ↔ 413		By similarity
	Disulfide bond	415 ↔ 424		By similarity
Natural variations
	Natural variant	78	1	D → E. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	I0CME7 [UniParc]. Last modified June 13, 2012. Version 1. Checksum: 57E4073753DDB803	FASTA	448	51,427
	10 20 30 40 50 60 MRAVVVVTGL VVVVVATALS LPNHDVKSAT SSRSSSDYQG SSGDDCDEGL PPPDQPFRVV 70 80 90 100 110 120 WNHPDNCERI KLHLPLDDYG IIFNKLRVFL GEEIQTLYDT GPWPYISETG KFINGGLPQS 130 140 150 160 170 180 FNHPDNDGET QRILKKHRPE NFTGLGVLDF ETWRAIYSTN FGPMTIYQNE SVKLVKEQHP 190 200 210 220 230 240 DYDQKKLTKV AEKEWQQAAK DIMSNKLKIA QEVMPRGHWG YYLYPRTWDN KRDTKFRNDK 250 260 270 280 290 300 INWLWRQSTG LYPSIYIYDF SKTESAITKF VSDTVGEAVR VQKEFSPPNT PIYPYVMFQT 310 320 330 340 350 360 MDNIFHYEDH LKISLGLSAK MGAAGVVLWG TSKHYKESTR QWQCQQLQEH IRTVLGPLVK 370 380 390 400 410 420 NVTQMMTDCS RAICEGHGRC VHNSHDVILG ETESQRLSDL CSTRQSRFRD YHCRCYSAWE 430 440 GACCQTLRPS RCQKREQRNV HGGGDLID

References

[1]

"Recruitment of glycosyl hydrolase proteins in a cone snail venomous arsenal: further insights into biomolecular features of Conus venoms." Violette A., Leonardi A., Piquemal D., Terrat Y., Biass D., Dutertre S., Noguier F., Ducancel F., Stocklin R., Krizaj I., Favreau P. Mar. Drugs 10:258-280(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 34-43 AND 137-154, CATALYTIC ACTIVITY, IDENTIFICATION BY MASS SPECTROMETRY, VARIANT GLU-78, GLYCOSYLATION AT ASN-141 AND ASN-361. Tissue: Venom and Venom duct.

Cross-references

Sequence databases
EMBL GenBank DDBJ	JN697596 mRNA. Translation: AFH78528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PRINTS	PR00846. GLHYDRLASE56.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL_CONCN

Accession

Primary (citable) accession number: I0CME7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2012
Last sequence update:	June 13, 2012
Last modified:	March 6, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)