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I0C681 (I0C681_STAA5) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:ST398NM01_2124 EMBL AFH70310.1
OrganismStaphylococcus aureus subsp. aureus 71193 [Complete proteome] EMBL AFH70310.1
Taxonomic identifier1155084 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain171 – 376206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding204 – 25956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3301Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3431Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3431Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3451Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I0C681 [UniParc].

Last modified June 13, 2012. Version 1.
Checksum: 2378C1390248FA08

FASTA39344,612
        10         20         30         40         50         60 
MILLRFKDAN KSFNNWTKSI LIYIKVANPD ISLEENEMTK ENICIVFGGK SAEHEVSILT 

        70         80         90        100        110        120 
AQNVLNAIDK DKYHVDIIYI TNDGDWRKQN NITTEIKSTD ELHLENGEAL EISQLLKESS 

       130        140        150        160        170        180 
SGQPYDAVFP LLHGPNGEDG TIQGLFEVLD VPYVGNGVLS AASSMDKLVM KQLFEHRGLP 

       190        200        210        220        230        240 
QLPYISFLRS EYEKYEHNIL KLVNDKLNYP VFVKPANLGS SVGISKCNNE AELKEGIKEA 

       250        260        270        280        290        300 
FQFDRKLVIE QGVNAREIEV AVLGNDYPEA TWPGEVVKDV AFYDYKSKYK DGKVQLQIPA 

       310        320        330        340        350        360 
DLDEDVQLTL RNMALEAFKA TDCSGLVRAD FFVTEDNQIY INETNAMPGF TAFSMYPKLW 

       370        380        390 
ENMGLSYPEL ITKLIELAKE RHQDKQKNKY KID 

« Hide

References

[1]"Identification of a highly transmissible animal-independent Staphylococcus aureus ST398 clone with distinct genomic and cell adhesion properties."
Uhlemann A.C., Porcella S.F., Trivedi S., Sullivan S.B., Hafer C., Kennedy A.D., Barbian K.D., McCarthy A.J., Street C., Hirschberg D.L., Lipkin W.I., Lindsay J.A., DeLeo F.R., Lowy F.D.
MBio 3:e00027-12(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 71193 EMBL AFH70310.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003045 Genomic DNA. Translation: AFH70310.1.
RefSeqYP_006196153.1. NC_017673.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFH70310; AFH70310; ST398NM01_2124.
GeneID12731226.
KEGGsud:ST398NM01_2124.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI0C681_STAA5
AccessionPrimary (citable) accession number: I0C681
Entry history
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: June 11, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)