I0BSU2 (I0BSU2_9BACL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084

Gene names

ORF Names:

B2K_32840 EMBL AFH65439.1

Organism

Paenibacillus mucilaginosus K02 EMBL AFH65439.1

Taxonomic identifier

997761 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus › Paenibacillus mucilaginosus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084
Sequence similarities	Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Ontologies

Keywords
Ligand	Metal-binding PIRSR PIRSR001084-3 Zinc PIRSR PIRSR001084-3
Molecular function	Glycosidase PIRNR PIRNR001084 Hydrolase
Gene Ontology (GO)
Biological_process	galactose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-galactosidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	150	1	Proton donor By similarity PIRSR PIRSR001084-1
	Active site	307	1	Nucleophile By similarity PIRSR PIRSR001084-1
	Metal binding	115	1	Zinc By similarity PIRSR PIRSR001084-3
	Metal binding	155	1	Zinc By similarity PIRSR PIRSR001084-3
	Metal binding	157	1	Zinc By similarity PIRSR PIRSR001084-3
	Metal binding	160	1	Zinc By similarity PIRSR PIRSR001084-3
	Binding site	111	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	149	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	315	1	Substrate By similarity PIRSR PIRSR001084-2

Sequences

	Sequence		Length	Mass (Da)
	I0BSU2 [UniParc]. Last modified June 13, 2012. Version 1. Checksum: 2E580F5159B373D9	FASTA	691	77,944
	10 20 30 40 50 60 MINKKLPKMF YGGDYNPEQW DKPVWDEDMR MFQLAGIDVA TVNVFSWALL QPSENEYNFE 70 80 90 100 110 120 LLDEIMDKLH ENGLYACLAT STGAHPAWMA HRHPDVLRVD FQGRKRKFGG RHNSCPNSPT 130 140 150 160 170 180 YRKYARLLAG KLAERYKDHP ALLVWHVSNE YGGECYCDNC AAAFRVWLQQ RYGSLEAVNK 190 200 210 220 230 240 AWNTRFWGHT FYDWEEIVPP NELSEQWGPT RTNFQGISLD YTRFNSDSLL ECYKEEHEEL 250 260 270 280 290 300 KKHTPDIMVT TNLMGTFKPL DYFKWAKHMD IVSWDNYPAA DTPVSRTAMT HDLMRGLKDG 310 320 330 340 350 360 APFMLMEQTP SQQNWQPYNS LKRPGVMRLW SYQAVARGAD TVMFFQLRRS VGACEKYHGA 370 380 390 400 410 420 VIEHVGHEHT RVFRECAQLG AELQQLGDSL LDSRVPSRAA IVFDWENWWA VEFSSGPTVA 430 440 450 460 470 480 LQYVEEVHKY YDALYQANIQ VDMIGTDADL SGYDVVIAPV LYMVKSGYAK KVEEFVQGGG 490 500 510 520 530 540 TFLTTFFSGI VNENDIVTLG GYPGELRKVL GIWAEEIDAL LPGKQNRIVM KKELGSLQGE 550 560 570 580 590 600 YACEMLCDLI HSEGAEIIAE YGDDFYQGMP VVTANTFGQG QAWYLASSPE PSFLQGLLQQ 610 620 630 640 650 660 LCRDKGIEPL LETPAGVEVS RRRKDGKDYY FLLNHNAAPQ TVDAAALDAV DVLAGGKAGI 670 680 690 VELPAHGAVI LQAAASAEDT RSGSAQGVLS R

References

[1]	"Complete genome sequence of Paenibacillus mucilaginosus K02." Xiao B., Sun L., Xiao L., Lian B. Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: K02 EMBL AFH65439.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003422 Genomic DNA. Translation: AFH65439.1.
RefSeq	YP_006193204.1. NC_017672.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AFH65439; AFH65439; B2K_32840.
GeneID	12734669.
KEGG	pmw:B2K_32840.
Phylogenomic databases
KO	K12308.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR013739. Beta_galactosidase_C. IPR013738. Beta_galactosidase_Trimer. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

I0BSU2_9BACL

Accession

Primary (citable) accession number: I0BSU2

Entry history

Integrated into UniProtKB/TrEMBL:	June 13, 2012
Last sequence update:	June 13, 2012
Last modified:	April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)