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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Spirochaeta africana (strain ATCC 700263 / DSM 8902 / Z-7692)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85ATP; via amide nitrogenUniRule annotation1
Metal bindingi178Magnesium; catalyticUniRule annotation1
Sitei179Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei208Proton acceptorUniRule annotation1
Binding sitei307SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 152ATPUniRule annotation2
Nucleotide bindingi177 – 180ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Spiaf_2436Imported
OrganismiSpirochaeta africana (strain ATCC 700263 / DSM 8902 / Z-7692)Imported
Taxonomic identifieri889378 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeSpirochaeta
Proteomesi
  • UP000007383 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 384PFKInterPro annotationAdd BLAST306

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 208Substrate bindingUniRule annotation3
Regioni251 – 253Substrate bindingUniRule annotation3
Regioni360 – 363Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiHSGFIAC.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

H9ULS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDFTIESLG DSKISSPISY DKVPGHNVTN FVSDDEAIIY DVDLDPQRKE
60 70 80 90 100
TWCADESQLI QKAGPRAKIY FSPPHVHAGI VSCGGLCPGI NDVIRAVVRT
110 120 130 140 150
LWYRYGVRRI TGIRNGYKGL IPEYGIAPID LSPDLVDDIH KIGGSILGTS
160 170 180 190 200
RGGGERTEEI VDTIERMNLN ILFTIGGDGT QKGSLAIARE IERRGLKISI
210 220 230 240 250
IGIPKTIDND FKFIQRSFGF ETAVARATEA VTGAHVEAQS VINGIGLVKV
260 270 280 290 300
MGRESGFIAA YTALASHEAN FVLIPEVPFE LDGPNGLIAH LVERLERRSH
310 320 330 340 350
AVVIVAEGAG QTLLERDASK TDDSGNVVLG DIGLYLKEKI ASSFKKAGIH
360 370 380 390 400
QNLKYIDPSY MIRSSVAVPT DSVYCSQLGN NAAHAAMAGK TKTMIGLVNN
410 420 430 440 450
HYVHLPIELV VASRNHVDPQ SSLWRSVIEA THQPVMMTNT KEAIETTAEQ
460
KAKMDKNRGN S
Length:461
Mass (Da):50,127
Last modified:June 13, 2012 - v1
Checksum:i113E9FC204016335
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003282 Genomic DNA. Translation: AFG38467.1.
RefSeqiWP_014456449.1. NC_017098.1.

Genome annotation databases

EnsemblBacteriaiAFG38467; AFG38467; Spiaf_2436.
KEGGisfc:Spiaf_2436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003282 Genomic DNA. Translation: AFG38467.1.
RefSeqiWP_014456449.1. NC_017098.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFG38467; AFG38467; Spiaf_2436.
KEGGisfc:Spiaf_2436.

Phylogenomic databases

KOiK00850.
OMAiHSGFIAC.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiH9ULS4_SPIAZ
AccessioniPrimary (citable) accession number: H9ULS4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2012
Last sequence update: June 13, 2012
Last modified: November 2, 2016
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.