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Protein

Bifunctional abietadiene synthase, chloroplastic

Gene

LAS

Organism
Abies balsamea (Balsam fir) (Pinus balsamea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to abietadiene. The copalyl diphosphate (CPP) intermediate diffuses freely between the 2 active sites in the enzyme.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate = (+)-copalyl diphosphate.1 Publication
+-copalyl diphosphate = abieta-7,13-diene + diphosphate.1 Publication
+-copalyl diphosphate = neoabietadiene + diphosphate.1 Publication
+-copalyl diphosphate = abieta-8(14),12-diene + diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: oleoresin biosynthesis

This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi597Magnesium 1By similarity1
Metal bindingi597Magnesium 2By similarity1
Metal bindingi601Magnesium 1By similarity1
Metal bindingi601Magnesium 2By similarity1
Metal bindingi741Magnesium 3By similarity1
Metal bindingi745Magnesium 3By similarity1
Metal bindingi749Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00924.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional abietadiene synthase, chloroplastic
Alternative name(s):
Abietadiene cyclase
Diterpene synthase TPS1
Short name:
AbdiTPS1
Levopimaradiene/abietadiene synthase
Short name:
AbLAS
Including the following 2 domains:
Abietadiene synthase (EC:4.2.3.18)
Alternative name(s):
Levopimaradiene synthase (EC:4.2.3.32)
Neoabietadiene synthase (EC:4.2.3.132)
Copalyl diphosphate synthase (EC:5.5.1.12)
Gene namesi
Name:LAS
OrganismiAbies balsamea (Balsam fir) (Pinus balsamea)
Taxonomic identifieri90345 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei‹1 – 46ChloroplastSequence analysisAdd BLAST›46
ChainiPRO_000042333947 – 844Bifunctional abietadiene synthase, chloroplasticAdd BLAST798

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliH8ZM70.
SMRiH8ZM70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi378 – 381DXDD motifCurated4
Motifi597 – 601DDXXD motifCurated5

Domaini

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.Curated

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsd subfamily.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.130. 2 hits.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom_sf.
IPR034741. Terpene_cyclase_like_1_C.
IPR001906. Terpene_synth_N.
IPR036965. Terpene_synth_N_sf.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
PfamiView protein in Pfam
PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
SFLDiSFLDG01019. Terpene_Cyclase_Like_1_C_Termi. 1 hit.
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

H8ZM70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QSIPHFSTTL NAGSSARKRR SLYLRWGKGS NKIIACVGEG ATSVPYQSAE
60 70 80 90 100
KNDSLYSSTL VKREFPPGFW KDDLIDSLTS SHKVAASDEK RIETLISEIK
110 120 130 140 150
NMFRCMGYGE TNPSAYDTAW VARIPALDGS DNPHFPETVE WILQNQLKDG
160 170 180 190 200
SWGEGFYFLA YDRILATLAC IITLTLWRTG ETQVHKGIEF FRTQAGKMED
210 220 230 240 250
EADSHRPSGF EIVFPAMLKE AKILGLDLPY DLPFLKQIIE KREAKLKRIP
260 270 280 290 300
TDVLYALPTT LLYSLEGLQE IVDWQKIMKL QSKDGSFLSS PASTAAVFMR
310 320 330 340 350
TGNKKCLDFL NFVLKKFGNH VPCHYPLDLF ERLWAVDTVE RLGIDRHFKE
360 370 380 390 400
EIKEALDYVY SHWDERGIGW ARENPVPDID DTAMGLRILR LHGYNVSSDV
410 420 430 440 450
LKTFRDENGE FFCFLGQTQR GVTDMLNVNR CSHVSFPGET IMEEAKLCTE
460 470 480 490 500
RYLRNALENV DAFDKWAFKK NIRGEVEYAL KYPWHKSMPR LEARSYIENY
510 520 530 540 550
GPDDVWLGKT VYMMPYISNE KYLELAKLDF NKVQSIHQTE LQDLRRWWKS
560 570 580 590 600
SGFTDLNFTR ERVTEIYFSP ASFIFEPEFS KCREVYTKTS NFTVILDDLY
610 620 630 640 650
DAHGSLDDLK LFTESVKRWD LSLVDQMPQQ MKICFVGFYN TFNEIAKEGR
660 670 680 690 700
ESQGRDVLGY IQNVWKVQLE AYTKEAEWSE AKYVPSFNEY IENASVSIAL
710 720 730 740 750
GTVVLISALF TGEVLTDEVL SKIDRGSRFL QLMGLTGRLV NDTKTYQAER
760 770 780 790 800
GQGEVASAIQ CYMKDHPKIS EEEALKHVYT VMENSLEELN REFVNNKIPD
810 820 830 840
IYRRLVFETA RIMQLFYMQG DGLTLSHDME IKEHVKNCLF QPVA
Length:844
Mass (Da):97,273
Last modified:May 16, 2012 - v1
Checksum:i03CED90C8652E368
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JN254805 mRNA. Translation: AEL99950.1.

Similar proteinsi

Entry informationi

Entry nameiLAS_ABIBA
AccessioniPrimary (citable) accession number: H8ZM70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: May 16, 2012
Last modified: February 28, 2018
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program