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H8LXZ9 (H8LXZ9_SALTM) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162 EMBL AFD59981.1
ORF Names:UMN798_3364 EMBL AFD59981.1
OrganismSalmonella enterica subsp. enterica serovar Typhimurium str. 798 EMBL AFD59981.1
Taxonomic identifier1008297 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain125 – 310186ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding151 – 20757ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2811Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2831Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
H8LXZ9 [UniParc].

Last modified May 16, 2012. Version 1.
Checksum: 98AC5A80C230761F

FASTA31535,433
        10         20         30         40         50         60 
MIKLGIVMDP IAHINIKKDT SFAMLLEAQR RGYELHYMEM ADLYLINGEA RARTRTLSVE 

        70         80         90        100        110        120 
QNYDKWYEFG SEQEIKLADL DVILMRKDPP FDTEFIYATY ILERAEEEGT LIVNKPQSLR 

       130        140        150        160        170        180 
DCNEKLYTAW FADLTPETLV TRNKAQLKAF WEKHGDIIMK PLDGMGGASI FRVKEGDPNI 

       190        200        210        220        230        240 
GVIAETLTEL GNRYCMAQNY LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG 

       250        260        270        280        290        300 
RGEPRPLSES DWEIARRVGP TLKAKGLIFV GLDIIGDRLT EINVTSPTCV REIEAEYPIS 

       310 
ITGMLMDAIE ARLAK

« Hide

References

[1]"Characterization and Differential Gene Expression between Two Phenotypic Phase Variants in Salmonella enterica Serovar Typhimurium."
Patterson S.K., Borewicz K., Johnson T., Xu W., Isaacson R.E.
PLoS ONE 7:E43592-E43592(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 798 EMBL AFD59981.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003386 Genomic DNA. Translation: AFD59981.1.
RefSeqYP_005398446.1. NC_017046.1.

3D structure databases

ProteinModelPortalH8LXZ9.
SMRH8LXZ9. Positions 1-313.
ModBaseSearch...

Proteomic databases

PRIDEH8LXZ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFD59981; AFD59981; UMN798_3364.
GeneID12001519.
KEGGsef:UMN798_3364.

Phylogenomic databases

KOK01920.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH8LXZ9_SALTM
AccessionPrimary (citable) accession number: H8LXZ9
Entry history
Integrated into UniProtKB/TrEMBL: May 16, 2012
Last sequence update: May 16, 2012
Last modified: May 1, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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