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H8K8A9 (H8K8A9_RICAC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFC71502.1
Ordered Locus Names:MC5_06280 EMBL AFC71502.1
OrganismRickettsia australis (strain Cutlack) [Complete proteome] [HAMAP] EMBL AFC71502.1
Taxonomic identifier1105110 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain120 – 314195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding147 – 19852ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2671Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2811Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2811Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2831Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
H8K8A9 [UniParc].

Last modified May 16, 2012. Version 1.
Checksum: D4A9E25AEF581FD4

FASTA32035,634
        10         20         30         40         50         60 
MKYQTHWIEH SVVKILSTTG KKHIALVAGG MSAEREVSLV SSEGVHKALI ELGYKVTFID 

        70         80         90        100        110        120 
MGADIAVKLQ KIKPDIVFNC LHGTYGEDGC LPGLLNIMRI PYTHSGVLSS ALAFDKIHSS 

       130        140        150        160        170        180 
SWFVTNNINT AESIVVSKSD NIKTDPMKRP YVIKPLTQGS SIGVEVIFEE DDFNFADYDF 

       190        200        210        220        230        240 
PYGDQVVIER YIKGRELQVA VLNSKALGVL EIKLLKNRFY DYATKYTEGF AEHLCPAPLP 

       250        260        270        280        290        300 
ANLYEKLLIA SEKIYKTMNC KGPARAEFIL EEQTNKLYAL EINTHPGMTL LSIVPEIAAY 

       310        320 
AGINFTNLIE EIIKTASFES 

« Hide

References

[1]"Complete genome sequence of Rickettsia australis strain Cutlack."
Johnson S.L., Munk A.C., Han S., Bruce D.C., Dasch G.A.
Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Cutlack.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003338 Genomic DNA. Translation: AFC71502.1.
RefSeqYP_005415345.1. NC_017058.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFC71502; AFC71502; MC5_06280.
GeneID12018467.
KEGGrau:MC5_06280.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycRAUS1105110:GLJ8-1083-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH8K8A9_RICAC
AccessionPrimary (citable) accession number: H8K8A9
Entry history
Integrated into UniProtKB/TrEMBL: May 16, 2012
Last sequence update: May 16, 2012
Last modified: June 11, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)