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H8K110 (H8K110_VIBCL) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFC60063.1
ORF Names:O3Y_16223 EMBL AFC60063.1
OrganismVibrio cholerae IEC224 [Complete proteome] EMBL AFC60063.1
Taxonomic identifier1134456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain121 – 327207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding151 – 20656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2811Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2941Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2941Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2961Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
H8K110 [UniParc].

Last modified May 16, 2012. Version 1.
Checksum: B7581B22D766818B

FASTA33437,318
        10         20         30         40         50         60 
MTKTTILLLC GGGSSEHEIS LVSANYIQQQ LELTPEFHVI RVEMKKEGWF SEQGALVYLD 

        70         80         90        100        110        120 
TNSATLNSDK ASYPIDFVVP CIHGFPGETG DIQSMLELAG IPYLGCGPEA SANSFNKITS 

       130        140        150        160        170        180 
KLWYDALDIP NTPYLFLTQN TPSSIDKAKQ AFGHWGSIFV KAARQGSSVG CYKVTTEDQI 

       190        200        210        220        230        240 
APAIEAAFGF SEQVLVEQAV KPRELEVSAY EMNGKLYISK PGEVIAPEGT FYSYEEKYSA 

       250        260        270        280        290        300 
NSHARTVLEA ENLTEKHKEL IQTYAERVFI HMKLRHLSRI DFFLTQEGQI YLNEVNTFPG 

       310        320        330 
MTPISMFPKM LEHNGHRFSE FLVQCVTNTL VNAK 

« Hide

References

[1]"Complete Genome Sequence of a Sucrose-Nonfermenting Epidemic Strain of Vibrio cholerae O1 from Brazil."
Morais L.L., Garza D.R., Loureiro E.C., Nunes K.N., Vellasco R.S., da Silva C.P., Nunes M.R., Thompson C.C., Vicente A.C., Santos E.C.
J. Bacteriol. 194:2772-2772(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: IEC224 EMBL AFC60063.1.
[2]"Genome-Wide Study of the Defective Sucrose Fermenter Strain of Vibrio cholerae from the Latin American Cholera Epidemic."
Garza D.R., Thompson C.C., Loureiro E.C., Dutilh B.E., Inada D.T., Junior E.C., Cardoso J.F., Nunes M.R., de Lima C.P., Silvestre R.V., Nunes K.N., Santos E.C., Edwards R.A., Vicente A.C., de Sa Morais L.L.
PLoS ONE 7:E37283-E37283(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: IEC224 EMBL AFC60063.1.
[3]Garza D.R.Jr., Thompson C.C., Loureiro E.C.B., Dutilh B.E., Inada D.T., Junior E.C.S., Cardoso J.F., Nunes M.R.T., de Lima C.P.S., Vellasco R.S., Nunes K.N.B., Santos E.C.O., Edwards R.A., Vicente A.C.P., Morais L.L.C.S.
Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: IEC224 EMBL AFC60063.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003331 Genomic DNA. Translation: AFC60063.1.
RefSeqYP_005335051.1. NC_016945.1.

3D structure databases

ProteinModelPortalH8K110.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFC60063; AFC60063; O3Y_16223.
GeneID11912440.
KEGGvci:O3Y_16223.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycVCHO1134456:GLMN-3294-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH8K110_VIBCL
AccessionPrimary (citable) accession number: H8K110
Entry history
Integrated into UniProtKB/TrEMBL: May 16, 2012
Last sequence update: May 16, 2012
Last modified: February 19, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)