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H8J0W5

- H8J0W5_MYCIT

UniProt

H8J0W5 - H8J0W5_MYCIT

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Mycobacterium intracellulare MOTT-02
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (16 May 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei310 – 3101Coenzyme AUniRule annotation
    Binding sitei386 – 3861Substrate; via nitrogen amideUniRule annotation
    Binding sitei508 – 5081SubstrateUniRule annotation
    Binding sitei523 – 5231SubstrateUniRule annotation
    Active sitei525 – 5251UniRule annotation
    Binding sitei531 – 5311Coenzyme AUniRule annotation
    Binding sitei534 – 5341SubstrateUniRule annotation
    Metal bindingi545 – 5451Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi547 – 5471Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei592 – 5921Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMINT1138382:GLGO-416-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    ORF Names:OCO_04070Imported
    OrganismiMycobacterium intracellulare MOTT-02Imported
    Taxonomic identifieri1138382 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
    ProteomesiUP000008002: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliH8J0W5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni410 – 4156Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    KOiK01895.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    H8J0W5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTATNSAGPS SYPPSARFAE QANAGEELYR DAERDRLAFW AEQANRLSWA    50
    TPFTEVLDWS DAPFAKWFGD GKLNVAYNCV DRHVEAGHGD RVAIHWEGEP 100
    VGDHRSLTYS QLQTEVCKAA NALTDLGLVA GDRVAIYLPL IPEAVIAMLA 150
    CARLGIMHSV VFAGFTAKAL QARIADADAK LLITSDGQFR RGRPAPLKDA 200
    ADEAVSDPDS PIEHVVVVRR TGIDVSWNDD RDLWWHDVVD AASPEHTPQP 250
    FDAEQPLFLL YTSGTTGKPK GIVHTSGGYL THAAYTHYYV FDIKADSDVF 300
    WCTADIGWVT GHTYGVYGPL ANGVTEVLYE GTPDSPSQHR HFEIIEKYGV 350
    TIYYTAPTLI RTFMKWGREV PDAHDLSSLR LLGSVGEPIN PEAWRWYRKV 400
    IGADSLPIVD TWWQTETGAT MISPLPGIAA AKPGSAMRPL PGVSAKIVDD 450
    HGEELPPPGE DKGEHVTGYL VLDQPWPSML RGIWGDPQRY VETYWSRFAE 500
    QGWYFAGDSA YYDTEGDIWV VGRIDDVMNV SGHRLSTAEV ESALVGHAAV 550
    AEAAVVGATD ETTGQAICAF VVLCADFEVH DGIVDDLRVE VAREISPIAR 600
    PREVHVVPEL PKTRSGKIMR RLLRDIAENR ELGDTSTLLD PGVFDAIRAA 650
    K 651
    Length:651
    Mass (Da):71,545
    Last modified:May 16, 2012 - v1
    Checksum:iBCA7FF98808D6AC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003323 Genomic DNA. Translation: AFC46771.1.
    RefSeqiWP_014381701.1. NC_016947.1.
    YP_005341092.1. NC_016947.1.

    Genome annotation databases

    EnsemblBacteriaiAFC46771; AFC46771; OCO_04070.
    GeneIDi11901923.
    KEGGimit:OCO_04070.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003323 Genomic DNA. Translation: AFC46771.1 .
    RefSeqi WP_014381701.1. NC_016947.1.
    YP_005341092.1. NC_016947.1.

    3D structure databases

    ProteinModelPortali H8J0W5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFC46771 ; AFC46771 ; OCO_04070 .
    GeneIDi 11901923.
    KEGGi mit:OCO_04070.

    Phylogenomic databases

    KOi K01895.

    Enzyme and pathway databases

    BioCyci MINT1138382:GLGO-416-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete Genome Sequence of Mycobacterium intracellulare Clinical Strain MOTT-02."
      Kim B.J., Choi B.S., Lim J.S., Choi I.Y., Lee J.H., Chun J., Kook Y.H., Kim B.J.
      J. Bacteriol. 194:2771-2771(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MOTT-02Imported.

    Entry informationi

    Entry nameiH8J0W5_MYCIT
    AccessioniPrimary (citable) accession number: H8J0W5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 16, 2012
    Last sequence update: May 16, 2012
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3