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H8J0W5

- H8J0W5_MYCIT

UniProt

H8J0W5 - H8J0W5_MYCIT

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Protein
Acetyl-coenzyme A synthetase
Gene
acsA, OCO_04070
Organism
Mycobacterium intracellulare MOTT-02
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA By similarity.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei310 – 3101Coenzyme A By similarityUniRule annotation
Binding sitei386 – 3861Substrate; via nitrogen amide By similarityUniRule annotation
Binding sitei508 – 5081Substrate By similarityUniRule annotation
Binding sitei523 – 5231Substrate By similarityUniRule annotation
Active sitei525 – 5251 By similarityUniRule annotation
Binding sitei531 – 5311Coenzyme A By similarityUniRule annotation
Binding sitei534 – 5341Substrate By similarityUniRule annotation
Metal bindingi545 – 5451Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi547 – 5471Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygen By similarityUniRule annotation
Binding sitei592 – 5921Coenzyme A By similarityUniRule annotation

GO - Molecular functioni

  1. AMP binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciMINT1138382:GLGO-416-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsAUniRule annotation
ORF Names:OCO_04070Imported
OrganismiMycobacterium intracellulare MOTT-02Imported
Taxonomic identifieri1138382 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000008002: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171N6-acetyllysine By similarityUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliH8J0W5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni410 – 4156Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK01895.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H8J0W5-1 [UniParc]FASTAAdd to Basket

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MTATNSAGPS SYPPSARFAE QANAGEELYR DAERDRLAFW AEQANRLSWA    50
TPFTEVLDWS DAPFAKWFGD GKLNVAYNCV DRHVEAGHGD RVAIHWEGEP 100
VGDHRSLTYS QLQTEVCKAA NALTDLGLVA GDRVAIYLPL IPEAVIAMLA 150
CARLGIMHSV VFAGFTAKAL QARIADADAK LLITSDGQFR RGRPAPLKDA 200
ADEAVSDPDS PIEHVVVVRR TGIDVSWNDD RDLWWHDVVD AASPEHTPQP 250
FDAEQPLFLL YTSGTTGKPK GIVHTSGGYL THAAYTHYYV FDIKADSDVF 300
WCTADIGWVT GHTYGVYGPL ANGVTEVLYE GTPDSPSQHR HFEIIEKYGV 350
TIYYTAPTLI RTFMKWGREV PDAHDLSSLR LLGSVGEPIN PEAWRWYRKV 400
IGADSLPIVD TWWQTETGAT MISPLPGIAA AKPGSAMRPL PGVSAKIVDD 450
HGEELPPPGE DKGEHVTGYL VLDQPWPSML RGIWGDPQRY VETYWSRFAE 500
QGWYFAGDSA YYDTEGDIWV VGRIDDVMNV SGHRLSTAEV ESALVGHAAV 550
AEAAVVGATD ETTGQAICAF VVLCADFEVH DGIVDDLRVE VAREISPIAR 600
PREVHVVPEL PKTRSGKIMR RLLRDIAENR ELGDTSTLLD PGVFDAIRAA 650
K 651
Length:651
Mass (Da):71,545
Last modified:May 16, 2012 - v1
Checksum:iBCA7FF98808D6AC4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003323 Genomic DNA. Translation: AFC46771.1.
RefSeqiWP_014381701.1. NC_016947.1.
YP_005341092.1. NC_016947.1.

Genome annotation databases

EnsemblBacteriaiAFC46771; AFC46771; OCO_04070.
GeneIDi11901923.
KEGGimit:OCO_04070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003323 Genomic DNA. Translation: AFC46771.1 .
RefSeqi WP_014381701.1. NC_016947.1.
YP_005341092.1. NC_016947.1.

3D structure databases

ProteinModelPortali H8J0W5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFC46771 ; AFC46771 ; OCO_04070 .
GeneIDi 11901923.
KEGGi mit:OCO_04070.

Phylogenomic databases

KOi K01895.

Enzyme and pathway databases

BioCyci MINT1138382:GLGO-416-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete Genome Sequence of Mycobacterium intracellulare Clinical Strain MOTT-02."
    Kim B.J., Choi B.S., Lim J.S., Choi I.Y., Lee J.H., Chun J., Kook Y.H., Kim B.J.
    J. Bacteriol. 194:2771-2771(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MOTT-02Imported.

Entry informationi

Entry nameiH8J0W5_MYCIT
AccessioniPrimary (citable) accession number: H8J0W5
Entry historyi
Integrated into UniProtKB/TrEMBL: May 16, 2012
Last sequence update: May 16, 2012
Last modified: September 3, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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