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H8J0W5

- H8J0W5_MYCIT

UniProt

H8J0W5 - H8J0W5_MYCIT

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Mycobacterium intracellulare MOTT-02
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Note: Magnesium.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei310 – 3101Coenzyme AUniRule annotation
Binding sitei508 – 5081ATPUniRule annotation
Binding sitei523 – 5231ATPUniRule annotation
Binding sitei531 – 5311Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei534 – 5341ATPUniRule annotation
Metal bindingi545 – 5451Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi547 – 5471Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi386 – 3883ATPUniRule annotation
Nucleotide bindingi410 – 4156ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMINT1138382:GLGO-416-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
ORF Names:OCO_04070Imported
OrganismiMycobacterium intracellulare MOTT-02Imported
Taxonomic identifieri1138382 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000008002: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliH8J0W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 1934Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

KOiK01895.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H8J0W5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTATNSAGPS SYPPSARFAE QANAGEELYR DAERDRLAFW AEQANRLSWA
60 70 80 90 100
TPFTEVLDWS DAPFAKWFGD GKLNVAYNCV DRHVEAGHGD RVAIHWEGEP
110 120 130 140 150
VGDHRSLTYS QLQTEVCKAA NALTDLGLVA GDRVAIYLPL IPEAVIAMLA
160 170 180 190 200
CARLGIMHSV VFAGFTAKAL QARIADADAK LLITSDGQFR RGRPAPLKDA
210 220 230 240 250
ADEAVSDPDS PIEHVVVVRR TGIDVSWNDD RDLWWHDVVD AASPEHTPQP
260 270 280 290 300
FDAEQPLFLL YTSGTTGKPK GIVHTSGGYL THAAYTHYYV FDIKADSDVF
310 320 330 340 350
WCTADIGWVT GHTYGVYGPL ANGVTEVLYE GTPDSPSQHR HFEIIEKYGV
360 370 380 390 400
TIYYTAPTLI RTFMKWGREV PDAHDLSSLR LLGSVGEPIN PEAWRWYRKV
410 420 430 440 450
IGADSLPIVD TWWQTETGAT MISPLPGIAA AKPGSAMRPL PGVSAKIVDD
460 470 480 490 500
HGEELPPPGE DKGEHVTGYL VLDQPWPSML RGIWGDPQRY VETYWSRFAE
510 520 530 540 550
QGWYFAGDSA YYDTEGDIWV VGRIDDVMNV SGHRLSTAEV ESALVGHAAV
560 570 580 590 600
AEAAVVGATD ETTGQAICAF VVLCADFEVH DGIVDDLRVE VAREISPIAR
610 620 630 640 650
PREVHVVPEL PKTRSGKIMR RLLRDIAENR ELGDTSTLLD PGVFDAIRAA

K
Length:651
Mass (Da):71,545
Last modified:May 16, 2012 - v1
Checksum:iBCA7FF98808D6AC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003323 Genomic DNA. Translation: AFC46771.1.
RefSeqiWP_014381701.1. NC_016947.1.
YP_005341092.1. NC_016947.1.

Genome annotation databases

EnsemblBacteriaiAFC46771; AFC46771; OCO_04070.
GeneIDi11901923.
KEGGimit:OCO_04070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003323 Genomic DNA. Translation: AFC46771.1 .
RefSeqi WP_014381701.1. NC_016947.1.
YP_005341092.1. NC_016947.1.

3D structure databases

ProteinModelPortali H8J0W5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFC46771 ; AFC46771 ; OCO_04070 .
GeneIDi 11901923.
KEGGi mit:OCO_04070.

Phylogenomic databases

KOi K01895.

Enzyme and pathway databases

BioCyci MINT1138382:GLGO-416-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete Genome Sequence of Mycobacterium intracellulare Clinical Strain MOTT-02."
    Kim B.J., Choi B.S., Lim J.S., Choi I.Y., Lee J.H., Chun J., Kook Y.H., Kim B.J.
    J. Bacteriol. 194:2771-2771(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MOTT-02Imported.

Entry informationi

Entry nameiH8J0W5_MYCIT
AccessioniPrimary (citable) accession number: H8J0W5
Entry historyi
Integrated into UniProtKB/TrEMBL: May 16, 2012
Last sequence update: May 16, 2012
Last modified: November 26, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3