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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Mycobacterium intracellulare (strain ATCC 13950 / DSM 43223 / JCM 6384 / NCTC 13025 / 3600)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi326Magnesium or manganese 1UniRule annotation1
Metal bindingi338Magnesium or manganese 1UniRule annotation1
Metal bindingi338Magnesium or manganese 2UniRule annotation1
Metal bindingi340Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:OCU_36540Imported
OrganismiMycobacterium intracellulare (strain ATCC 13950 / DSM 43223 / JCM 6384 / NCTC 13025 / 3600)Imported
Taxonomic identifieri487521 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000008004 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini164 – 371ATP-graspInterPro annotationAdd BLAST208

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01921.
OMAiYETKYTE.
OrthoDBiPOG091H06GK.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

H8II66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNASDRSTPH PGSGGGERAR VAVVFGGRSN EHAISCVSAG SILRNLDPER
60 70 80 90 100
FEVVAIGITP EGSWVLTDGD PAALAITDRR LPEVTAESGT ELALPADPRR
110 120 130 140 150
GGQLVALPPG AGEVLASVDV VFPVLHGPYG EDGTIQGLLE LAGVPYVGAG
160 170 180 190 200
VLASAAGMDK EFTKKLFAAE GLPVGDYAVL RPSRARLRPE ECQRLGLPVF
210 220 230 240 250
VKPARGGSSI GVSRVASWDQ LDAAVAAARR HDPKVIVEAA IVGRELECGV
260 270 280 290 300
LEMPDGTVEA SALGEIRVAG VRGREDSFYD FATKYLDDAA ELDVPAKVDD
310 320 330 340 350
EIADTVRQLA VRAFKAIDCQ GLARVDFFLT EDGPVLNEIN TMPGFTTISM
360 370 380
YPRMWAASGV DYPTLLATMV ETALARGVGL R
Length:381
Mass (Da):40,130
Last modified:May 16, 2012 - v1
Checksum:iB5ED3C3648AFF434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003322 Genomic DNA. Translation: AFC44873.1.
RefSeqiWP_014380729.1. NC_016946.1.

Genome annotation databases

EnsemblBacteriaiAFC44873; AFC44873; OCU_36540.
GeneIDi11910401.
KEGGimia:OCU_36540.
PATRICifig|487521.10.peg.3661.

Similar proteinsi

Entry informationi

Entry nameiH8II66_MYCIA
AccessioniPrimary (citable) accession number: H8II66
Entry historyiIntegrated into UniProtKB/TrEMBL: May 16, 2012
Last sequence update: May 16, 2012
Last modified: September 27, 2017
This is version 42 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported