H7C794 (H7C794_ENTFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase IV, B subunit EMBL AAO81398.1

Gene names

Name:	parE EMBL AAO81398.1
Ordered Locus Names:	EF_1615 EMBL AAO81398.1

Organism

Enterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP] EMBL AAO81398.1

Taxonomic identifier

226185 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus › Enterococcus faecalis

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the type II topoisomerase family. RuleBase RU000380 Contains 1 Toprim domain. SAAS SAAS002288

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS002288 RuleBase RU000380 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000380 SAAS SAAS002288
Technical term	3D-structure PDB 4HZ5 Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	H7C794 [UniParc]. Last modified April 18, 2012. Version 1. Checksum: A4C79A5FB0915BFA	FASTA	685	76,257
	10 20 30 40 50 60 MAKKINNEYN DASIQVLEGL EAVRKRPGMY IGSTDSRGLH HLVYEIVDNA VDEALSGYGN 70 80 90 100 110 120 EINVTIQKDN SICVADSGRG MPTGMHASGI PTVEVIFTVL HAGGKFGQGG YKTSGGLHGV 130 140 150 160 170 180 GASVVNALSK WLEVHIVRDG VEYMERFEDG GKPVGTLKKI GKTKKRNGTS VTFLPDDTIF 190 200 210 220 230 240 STTNFSYEIL AERLRESAFL LKGVKITLTD ERGEEPKEEV FHYEEGIKEF VAYLNEEKDT 250 260 270 280 290 300 LTPVVYFSGA KEGIEVELAY QYNDGYSENV LSFVNNVRTK DGGTHEVGMK TSMTKAYNEY 310 320 330 340 350 360 ARKVGLLKEK DKNLEGSDFR EGLAAVLSIR VPENLLQFEG QTKGKLGTPL ARTVVDNVVG 370 380 390 400 410 420 EQMGFYLQEN SEMSQSLIRK AIKAREAREA ARKAREESRN GKKRKKGESL LSGKLTPAQS 430 440 450 460 470 480 RNPKKNELYL VEGDSAGGSA KQGRDRKFQA ILPLRGKVIN TEKAKMQDIL KNEEINTMIY 490 500 510 520 530 540 TIGAGVGPEF SIEDCNYDKI IIMTDADTDG AHIQVLLLTF FYRYMKPLIE AGKVYIALPP 550 560 570 580 590 600 LYKVSKGTGK KSVIEYAWTD GELAEVIDKV GKGYMLQRYK GLGEMNAEQL WETTMDPETR 610 620 630 640 650 660 TLIRVRIDDA AQAERRVTTL MGDKVEPRRK WIEQHVQFTL EEDGSILDRS EEDTSAPTGE 670 680 SLLDAEKTKE AEQTDDTEIS LFDIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.
[2]	"Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity." Tari L.W., Trzoss M., Bensen D.C., Li X., Chen Z., Lam T., Zhang J., Creighton C.J., Cunningham M.L., Kwan B., Stidham M., Shaw K.J., Lightstone F.C., Wong S.E., Nguyen T.B., Nix J., Finn J. Bioorg. Med. Chem. Lett. 23:1529-1536(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 19-225.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016830 Genomic DNA. Translation: AAO81398.1.

RefSeq

NP_815328.1. NC_004668.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HZ5	X-ray	2.70	A/B/C/D/E/F/G/H/J	19-225	[»]

HSSP

HSSP built from PDB template 1AJ6 based on UniProtKB P06982.

ProteinModelPortal

H7C794.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAO81398; AAO81398; EF_1615.

GeneID

1200513.

KEGG

efa:EF1615.

Phylogenomic databases

KO

K02622.

OMA

TDGTGLH.

Family and domain databases

Gene3D

3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.

InterPro

IPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005740. TopoIV_B_Gpos.
IPR006171. Toprim_domain.
[Graphical view]

Pfam

PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]

PRINTS

PR00418. TPI2FAMILY.

SMART

SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]

SUPFAM

SSF55874. ATP_bd_ATPase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
SSF56719. Topo_IIA_cen. 1 hit.

TIGRFAMs

TIGR01058. parE_Gpos. 1 hit.

PROSITE

PS00177. TOPOISOMERASE_II. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

H7C794_ENTFA

Accession

Primary (citable) accession number: H7C794

Entry history

Integrated into UniProtKB/TrEMBL:	April 18, 2012
Last sequence update:	April 18, 2012
Last modified:	May 1, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)