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H7C794

- H7C794_ENTFA

UniProt

H7C794 - H7C794_ENTFA

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Protein

DNA topoisomerase 4 subunit B

Gene
parE, EF_1615, I574_02005, OO5_01863
Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91ATP By similarityUniRule annotation
Binding sitei49 – 491ATP By similarityUniRule annotation
Binding sitei76 – 761ATP By similarityUniRule annotation
Binding sitei343 – 3431ATP By similarityUniRule annotation
Metal bindingi432 – 4321Magnesium 1; catalytic By similarityUniRule annotation
Sitei457 – 4571Interaction with DNA By similarityUniRule annotation
Sitei460 – 4601Interaction with DNA By similarityUniRule annotation
Metal bindingi505 – 5051Magnesium 1; catalytic By similarityUniRule annotation
Metal bindingi505 – 5051Magnesium 2 By similarityUniRule annotation
Metal bindingi507 – 5071Magnesium 2 By similarityUniRule annotation
Sitei512 – 5121Interaction with DNA By similarityUniRule annotation
Sitei628 – 6281Interaction with DNA By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1227ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-HAMAP
  2. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, DNA-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-1599-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit B
Gene namesi
Name:parEUniRule annotationImported
Ordered Locus Names:EF_1615Imported
ORF Names:I574_02005Imported, OO5_01863Imported
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)Imported
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE By similarity.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HZ5X-ray2.70A/B/C/D/E/F/G/H/J19-225[»]

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini426 – 540115Toprim By similarityUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation
Contains Toprim domain.SAAS annotations

Phylogenomic databases

KOiK02622.
OMAiPVGKHAM.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H7C794-1 [UniParc]FASTAAdd to Basket

« Hide

MAKKINNEYN DASIQVLEGL EAVRKRPGMY IGSTDSRGLH HLVYEIVDNA    50
VDEALSGYGN EINVTIQKDN SICVADSGRG MPTGMHASGI PTVEVIFTVL 100
HAGGKFGQGG YKTSGGLHGV GASVVNALSK WLEVHIVRDG VEYMERFEDG 150
GKPVGTLKKI GKTKKRNGTS VTFLPDDTIF STTNFSYEIL AERLRESAFL 200
LKGVKITLTD ERGEEPKEEV FHYEEGIKEF VAYLNEEKDT LTPVVYFSGA 250
KEGIEVELAY QYNDGYSENV LSFVNNVRTK DGGTHEVGMK TSMTKAYNEY 300
ARKVGLLKEK DKNLEGSDFR EGLAAVLSIR VPENLLQFEG QTKGKLGTPL 350
ARTVVDNVVG EQMGFYLQEN SEMSQSLIRK AIKAREAREA ARKAREESRN 400
GKKRKKGESL LSGKLTPAQS RNPKKNELYL VEGDSAGGSA KQGRDRKFQA 450
ILPLRGKVIN TEKAKMQDIL KNEEINTMIY TIGAGVGPEF SIEDCNYDKI 500
IIMTDADTDG AHIQVLLLTF FYRYMKPLIE AGKVYIALPP LYKVSKGTGK 550
KSVIEYAWTD GELAEVIDKV GKGYMLQRYK GLGEMNAEQL WETTMDPETR 600
TLIRVRIDDA AQAERRVTTL MGDKVEPRRK WIEQHVQFTL EEDGSILDRS 650
EEDTSAPTGE SLLDAEKTKE AEQTDDTEIS LFDIE 685
Length:685
Mass (Da):76,257
Last modified:April 18, 2012 - v1
Checksum:iA4C79A5FB0915BFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO81398.1.
AHYN01000009 Genomic DNA. Translation: EOT50099.1.
ASWP01000006 Genomic DNA. Translation: EOT84825.1.
RefSeqiNP_815328.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO81398; AAO81398; EF_1615.
EOT50099; EOT50099; OO5_01863.
EOT84825; EOT84825; I574_02005.
GeneIDi1200513.
KEGGiefa:EF1615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO81398.1 .
AHYN01000009 Genomic DNA. Translation: EOT50099.1 .
ASWP01000006 Genomic DNA. Translation: EOT84825.1 .
RefSeqi NP_815328.1. NC_004668.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HZ5 X-ray 2.70 A/B/C/D/E/F/G/H/J 19-225 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO81398 ; AAO81398 ; EF_1615 .
EOT50099 ; EOT50099 ; OO5_01863 .
EOT84825 ; EOT84825 ; I574_02005 .
GeneIDi 1200513.
KEGGi efa:EF1615.

Phylogenomic databases

KOi K02622.
OMAi PVGKHAM.
OrthoDBi EOG6P334W.

Enzyme and pathway databases

BioCyci EFAE226185:GHI1-1599-MONOMER.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_00939. ParE_type2.
InterProi IPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01058. parE_Gpos. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583 and V583Imported.
  2. "Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity."
    Tari L.W., Trzoss M., Bensen D.C., Li X., Chen Z., Lam T., Zhang J., Creighton C.J., Cunningham M.L., Kwan B., Stidham M., Shaw K.J., Lightstone F.C., Wong S.E., Nguyen T.B., Nix J., Finn J.
    Bioorg. Med. Chem. Lett. 23:1529-1536(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 19-225.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: V583Imported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: V583Imported.

Entry informationi

Entry nameiH7C794_ENTFA
AccessioniPrimary (citable) accession number: H7C794
Entry historyi
Integrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: September 3, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteome

External Data

Dasty 3

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