Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA (PubMed:23352267). Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation

Enzyme regulationi

Pyrrolopyrimidines inhibit both GyrB and its paralog in topoisomerase IV (parE) (PubMed:23352267).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi432 – 4321Magnesium 1; catalyticPROSITE-ProRule annotation
Metal bindingi505 – 5051Magnesium 1; catalyticPROSITE-ProRule annotation
Metal bindingi505 – 5051Magnesium 2PROSITE-ProRule annotation
Metal bindingi507 – 5071Magnesium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-1599-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
Ordered Locus Names:EF_1615
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 685685DNA topoisomerase 4 subunit BPRO_0000435622Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei457 – 4571Interaction with DNAPROSITE-ProRule annotation
Sitei460 – 4601Interaction with DNAPROSITE-ProRule annotation

Protein-protein interaction databases

STRINGi226185.EF1615.

Structurei

Secondary structure

1
685
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 256Combined sources
Helixi28 – 314Combined sources
Helixi36 – 5621Combined sources
Beta strandi60 – 667Combined sources
Beta strandi72 – 765Combined sources
Beta strandi89 – 913Combined sources
Helixi92 – 987Combined sources
Helixi122 – 1276Combined sources
Beta strandi129 – 13810Combined sources
Beta strandi141 – 1488Combined sources
Turni149 – 1513Combined sources
Beta strandi152 – 16211Combined sources
Beta strandi167 – 1759Combined sources
Turni177 – 1793Combined sources
Helixi187 – 19913Combined sources
Beta strandi205 – 2106Combined sources
Beta strandi212 – 2154Combined sources
Beta strandi217 – 2215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HZ5X-ray2.70A/B/C/D/E/F/G/H/J19-225[»]
ProteinModelPortaliH7C794.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini426 – 540115ToprimPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 1 subfamily.UniRule annotation
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C7D. Bacteria.
COG0187. LUCA.
KOiK02622.
OMAiKFRMGED.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE_type2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H7C794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKINNEYN DASIQVLEGL EAVRKRPGMY IGSTDSRGLH HLVYEIVDNA
60 70 80 90 100
VDEALSGYGN EINVTIQKDN SICVADSGRG MPTGMHASGI PTVEVIFTVL
110 120 130 140 150
HAGGKFGQGG YKTSGGLHGV GASVVNALSK WLEVHIVRDG VEYMERFEDG
160 170 180 190 200
GKPVGTLKKI GKTKKRNGTS VTFLPDDTIF STTNFSYEIL AERLRESAFL
210 220 230 240 250
LKGVKITLTD ERGEEPKEEV FHYEEGIKEF VAYLNEEKDT LTPVVYFSGA
260 270 280 290 300
KEGIEVELAY QYNDGYSENV LSFVNNVRTK DGGTHEVGMK TSMTKAYNEY
310 320 330 340 350
ARKVGLLKEK DKNLEGSDFR EGLAAVLSIR VPENLLQFEG QTKGKLGTPL
360 370 380 390 400
ARTVVDNVVG EQMGFYLQEN SEMSQSLIRK AIKAREAREA ARKAREESRN
410 420 430 440 450
GKKRKKGESL LSGKLTPAQS RNPKKNELYL VEGDSAGGSA KQGRDRKFQA
460 470 480 490 500
ILPLRGKVIN TEKAKMQDIL KNEEINTMIY TIGAGVGPEF SIEDCNYDKI
510 520 530 540 550
IIMTDADTDG AHIQVLLLTF FYRYMKPLIE AGKVYIALPP LYKVSKGTGK
560 570 580 590 600
KSVIEYAWTD GELAEVIDKV GKGYMLQRYK GLGEMNAEQL WETTMDPETR
610 620 630 640 650
TLIRVRIDDA AQAERRVTTL MGDKVEPRRK WIEQHVQFTL EEDGSILDRS
660 670 680
EEDTSAPTGE SLLDAEKTKE AEQTDDTEIS LFDIE
Length:685
Mass (Da):76,257
Last modified:April 18, 2012 - v1
Checksum:iA4C79A5FB0915BFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO81398.1.
RefSeqiNP_815328.1. NC_004668.1.
WP_002381887.1. NZ_KE136528.1.

Genome annotation databases

EnsemblBacteriaiAAO81398; AAO81398; EF_1615.
GeneIDi1200513.
KEGGiefa:EF1615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO81398.1.
RefSeqiNP_815328.1. NC_004668.1.
WP_002381887.1. NZ_KE136528.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HZ5X-ray2.70A/B/C/D/E/F/G/H/J19-225[»]
ProteinModelPortaliH7C794.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226185.EF1615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO81398; AAO81398; EF_1615.
GeneIDi1200513.
KEGGiefa:EF1615.

Phylogenomic databases

eggNOGiENOG4105C7D. Bacteria.
COG0187. LUCA.
KOiK02622.
OMAiKFRMGED.
OrthoDBiEOG6P334W.

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-1599-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE_type2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.
  2. "Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity."
    Tari L.W., Trzoss M., Bensen D.C., Li X., Chen Z., Lam T., Zhang J., Creighton C.J., Cunningham M.L., Kwan B., Stidham M., Shaw K.J., Lightstone F.C., Wong S.E., Nguyen T.B., Nix J., Finn J.
    Bioorg. Med. Chem. Lett. 23:1529-1536(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 19-225 IN COMPLEX WITH INHIBITOR, FUNCTION, ENZYME REGULATION.

Entry informationi

Entry nameiPARE_ENTFA
AccessioniPrimary (citable) accession number: H7C794
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: April 18, 2012
Last modified: April 13, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.