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H7C794 (H7C794_ENTFA) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 4 subunit B HAMAP-Rule MF_00939

EC=5.99.1.3 HAMAP-Rule MF_00939
Alternative name(s):
Topoisomerase IV subunit B HAMAP-Rule MF_00939
Gene names
Name:parE HAMAP-Rule MF_00939 EMBL AAO81398.1
Ordered Locus Names:EF_1615 EMBL AAO81398.1
ORF Names:I574_02005 EMBL EOT84825.1, OO5_01863 EMBL EOT50099.1
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP] EMBL AAO81398.1
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length685 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity. HAMAP-Rule MF_00939

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00939

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. HAMAP-Rule MF_00939

Subunit structure

Heterotetramer composed of ParC and ParE By similarity. HAMAP-Rule MF_00939

Sequence similarities

Belongs to the type II topoisomerase family. RuleBase RU000380

Belongs to the type II topoisomerase family. ParE type 2 subfamily. HAMAP-Rule MF_00939

Contains 1 Toprim domain. HAMAP-Rule MF_00939

Contains Toprim domain. SAAS SAAS002288

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain426 – 540115Toprim By similarity HAMAP-Rule MF_00939
Nucleotide binding116 – 1227ATP By similarity HAMAP-Rule MF_00939

Sites

Metal binding4321Magnesium 1; catalytic By similarity HAMAP-Rule MF_00939
Metal binding5051Magnesium 1; catalytic By similarity HAMAP-Rule MF_00939
Metal binding5051Magnesium 2 By similarity HAMAP-Rule MF_00939
Metal binding5071Magnesium 2 By similarity HAMAP-Rule MF_00939
Binding site91ATP By similarity HAMAP-Rule MF_00939
Binding site491ATP By similarity HAMAP-Rule MF_00939
Binding site761ATP By similarity HAMAP-Rule MF_00939
Binding site3431ATP By similarity HAMAP-Rule MF_00939
Site4571Interaction with DNA By similarity HAMAP-Rule MF_00939
Site4601Interaction with DNA By similarity HAMAP-Rule MF_00939
Site5121Interaction with DNA By similarity HAMAP-Rule MF_00939
Site6281Interaction with DNA By similarity HAMAP-Rule MF_00939

Sequences

Sequence LengthMass (Da)Tools
H7C794 [UniParc].

Last modified April 18, 2012. Version 1.
Checksum: A4C79A5FB0915BFA

FASTA68576,257
        10         20         30         40         50         60 
MAKKINNEYN DASIQVLEGL EAVRKRPGMY IGSTDSRGLH HLVYEIVDNA VDEALSGYGN 

        70         80         90        100        110        120 
EINVTIQKDN SICVADSGRG MPTGMHASGI PTVEVIFTVL HAGGKFGQGG YKTSGGLHGV 

       130        140        150        160        170        180 
GASVVNALSK WLEVHIVRDG VEYMERFEDG GKPVGTLKKI GKTKKRNGTS VTFLPDDTIF 

       190        200        210        220        230        240 
STTNFSYEIL AERLRESAFL LKGVKITLTD ERGEEPKEEV FHYEEGIKEF VAYLNEEKDT 

       250        260        270        280        290        300 
LTPVVYFSGA KEGIEVELAY QYNDGYSENV LSFVNNVRTK DGGTHEVGMK TSMTKAYNEY 

       310        320        330        340        350        360 
ARKVGLLKEK DKNLEGSDFR EGLAAVLSIR VPENLLQFEG QTKGKLGTPL ARTVVDNVVG 

       370        380        390        400        410        420 
EQMGFYLQEN SEMSQSLIRK AIKAREAREA ARKAREESRN GKKRKKGESL LSGKLTPAQS 

       430        440        450        460        470        480 
RNPKKNELYL VEGDSAGGSA KQGRDRKFQA ILPLRGKVIN TEKAKMQDIL KNEEINTMIY 

       490        500        510        520        530        540 
TIGAGVGPEF SIEDCNYDKI IIMTDADTDG AHIQVLLLTF FYRYMKPLIE AGKVYIALPP 

       550        560        570        580        590        600 
LYKVSKGTGK KSVIEYAWTD GELAEVIDKV GKGYMLQRYK GLGEMNAEQL WETTMDPETR 

       610        620        630        640        650        660 
TLIRVRIDDA AQAERRVTTL MGDKVEPRRK WIEQHVQFTL EEDGSILDRS EEDTSAPTGE 

       670        680 
SLLDAEKTKE AEQTDDTEIS LFDIE 

« Hide

References

« Hide 'large scale' references
[1]"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis."
Paulsen I., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L., Brinkac L., Beanan M., Daugherty S., DeBoy R.T., Durkin S. expand/collapse author list , Kolonay J., Madupu R., Nelson W., Vamathevan J., Tran B., Upton J., Hansen T., Shetty J., Khouri H., Utterback T., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.
Science 299:2071-2074(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700802 / V583 and V583 EMBL AAO81398.1.
[2]"Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity."
Tari L.W., Trzoss M., Bensen D.C., Li X., Chen Z., Lam T., Zhang J., Creighton C.J., Cunningham M.L., Kwan B., Stidham M., Shaw K.J., Lightstone F.C., Wong S.E., Nguyen T.B., Nix J., Finn J.
Bioorg. Med. Chem. Lett. 23:1529-1536(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 19-225.
[3]"The Genome Sequence of Enterococcus faecalis V583 (Illumina only assembly)."
The Broad Institute Genomics Platform, The Broad Institute Genome Sequencing Center for Infectious Disease
Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J. expand/collapse author list , Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.
Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: V583 EMBL EOT50099.1.
[4]"The Genome Sequence of Enterococcus faecalis V583 (PacBio/Illumina hybrid assembly)."
The Broad Institute Genomics Platform, The Broad Institute Genome Sequencing Center for Infectious Disease
Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J. expand/collapse author list , Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.
Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: V583 EMBL EOT84825.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016830 Genomic DNA. Translation: AAO81398.1.
AHYN01000009 Genomic DNA. Translation: EOT50099.1.
ASWP01000006 Genomic DNA. Translation: EOT84825.1.
RefSeqNP_815328.1. NC_004668.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HZ5X-ray2.70A/B/C/D/E/F/G/H/J19-225[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO81398; AAO81398; EF_1615.
EOT50099; EOT50099; OO5_01863.
EOT84825; EOT84825; I574_02005.
GeneID1200513.
KEGGefa:EF1615.

Phylogenomic databases

KOK02622.
OMAPVGKHAM.
OrthoDBEOG6P334W.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-1599-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_00939. ParE_type2.
InterProIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005740. TopoIV_B_Gpos.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01058. parE_Gpos. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH7C794_ENTFA
AccessionPrimary (citable) accession number: H7C794
Entry history
Integrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: July 9, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)