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H7C794

- H7C794_ENTFA

UniProt

H7C794 - H7C794_ENTFA

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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Note: Magnesium. Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) and Ca(2+).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91ATPUniRule annotation
Binding sitei49 – 491ATPUniRule annotation
Binding sitei76 – 761ATPUniRule annotation
Binding sitei343 – 3431ATPUniRule annotation
Metal bindingi432 – 4321Magnesium 1; catalyticUniRule annotation
Sitei457 – 4571Interaction with DNAUniRule annotation
Sitei460 – 4601Interaction with DNAUniRule annotation
Metal bindingi505 – 5051Magnesium 1; catalyticUniRule annotation
Metal bindingi505 – 5051Magnesium 2UniRule annotation
Metal bindingi507 – 5071Magnesium 2UniRule annotation
Sitei512 – 5121Interaction with DNAUniRule annotation
Sitei628 – 6281Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1227ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-HAMAP
  2. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, DNA-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-1599-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotationImported
Ordered Locus Names:EF_1615Imported
ORF Names:I574_02005Imported, OO5_01863Imported
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)Imported
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HZ5X-ray2.70A/B/C/D/E/F/G/H/J19-225[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini426 – 540115ToprimUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotation
Belongs to the type II topoisomerase family. ParE type 2 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation
Contains Toprim domain.SAAS annotation

Phylogenomic databases

KOiK02622.
OMAiPVGKHAM.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H7C794-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKKINNEYN DASIQVLEGL EAVRKRPGMY IGSTDSRGLH HLVYEIVDNA
60 70 80 90 100
VDEALSGYGN EINVTIQKDN SICVADSGRG MPTGMHASGI PTVEVIFTVL
110 120 130 140 150
HAGGKFGQGG YKTSGGLHGV GASVVNALSK WLEVHIVRDG VEYMERFEDG
160 170 180 190 200
GKPVGTLKKI GKTKKRNGTS VTFLPDDTIF STTNFSYEIL AERLRESAFL
210 220 230 240 250
LKGVKITLTD ERGEEPKEEV FHYEEGIKEF VAYLNEEKDT LTPVVYFSGA
260 270 280 290 300
KEGIEVELAY QYNDGYSENV LSFVNNVRTK DGGTHEVGMK TSMTKAYNEY
310 320 330 340 350
ARKVGLLKEK DKNLEGSDFR EGLAAVLSIR VPENLLQFEG QTKGKLGTPL
360 370 380 390 400
ARTVVDNVVG EQMGFYLQEN SEMSQSLIRK AIKAREAREA ARKAREESRN
410 420 430 440 450
GKKRKKGESL LSGKLTPAQS RNPKKNELYL VEGDSAGGSA KQGRDRKFQA
460 470 480 490 500
ILPLRGKVIN TEKAKMQDIL KNEEINTMIY TIGAGVGPEF SIEDCNYDKI
510 520 530 540 550
IIMTDADTDG AHIQVLLLTF FYRYMKPLIE AGKVYIALPP LYKVSKGTGK
560 570 580 590 600
KSVIEYAWTD GELAEVIDKV GKGYMLQRYK GLGEMNAEQL WETTMDPETR
610 620 630 640 650
TLIRVRIDDA AQAERRVTTL MGDKVEPRRK WIEQHVQFTL EEDGSILDRS
660 670 680
EEDTSAPTGE SLLDAEKTKE AEQTDDTEIS LFDIE
Length:685
Mass (Da):76,257
Last modified:April 18, 2012 - v1
Checksum:iA4C79A5FB0915BFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO81398.1.
AHYN01000009 Genomic DNA. Translation: EOT50099.1.
ASWP01000006 Genomic DNA. Translation: EOT84825.1.
RefSeqiNP_815328.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO81398; AAO81398; EF_1615.
EOT50099; EOT50099; OO5_01863.
EOT84825; EOT84825; I574_02005.
GeneIDi1200513.
KEGGiefa:EF1615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO81398.1 .
AHYN01000009 Genomic DNA. Translation: EOT50099.1 .
ASWP01000006 Genomic DNA. Translation: EOT84825.1 .
RefSeqi NP_815328.1. NC_004668.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HZ5 X-ray 2.70 A/B/C/D/E/F/G/H/J 19-225 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO81398 ; AAO81398 ; EF_1615 .
EOT50099 ; EOT50099 ; OO5_01863 .
EOT84825 ; EOT84825 ; I574_02005 .
GeneIDi 1200513.
KEGGi efa:EF1615.

Phylogenomic databases

KOi K02622.
OMAi PVGKHAM.
OrthoDBi EOG6P334W.

Enzyme and pathway databases

BioCyci EFAE226185:GHI1-1599-MONOMER.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_00939. ParE_type2.
InterProi IPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01058. parE_Gpos. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583Imported and V583Imported.
  2. "Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity."
    Tari L.W., Trzoss M., Bensen D.C., Li X., Chen Z., Lam T., Zhang J., Creighton C.J., Cunningham M.L., Kwan B., Stidham M., Shaw K.J., Lightstone F.C., Wong S.E., Nguyen T.B., Nix J., Finn J.
    Bioorg. Med. Chem. Lett. 23:1529-1536(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 19-225.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: V583Imported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: V583Imported.

Entry informationi

Entry nameiH7C794_ENTFA
AccessioniPrimary (citable) accession number: H7C794
Entry historyi
Integrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: November 26, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteomeImported

External Data

Dasty 3