Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable DNA ligase

Gene

ligB2

Organism
Blastococcus saxobsidens (strain DD2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei210 – 2101ATPUniRule annotation
Active sitei212 – 2121N6-AMP-lysine intermediateUniRule annotation
Binding sitei217 – 2171ATPUniRule annotation
Binding sitei232 – 2321ATPUniRule annotation
Binding sitei261 – 2611ATPUniRule annotation
Binding sitei302 – 3021ATPUniRule annotation
Binding sitei378 – 3781ATPUniRule annotation
Binding sitei384 – 3841ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSAX1146883:GL9E-2786-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligB2Imported
Synonyms:ligUniRule annotation
Ordered Locus Names:BLASA_2878Imported
OrganismiBlastococcus saxobsidens (strain DD2)Imported
Taxonomic identifieri1146883 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeGeodermatophilaceaeBlastococcus
ProteomesiUP000007517: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.
OMAiGRPRPFQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H6RLM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLAEVVAAS TAVAAARGRK AKAGLIAGAL RLADGDEVPA VTAWLSGEPL
60 70 80 90 100
QRRLGIGWRS LAALTGGAAA ESTLTVADVD AAFAELATTS GPGSAARREE
110 120 130 140 150
LLRGVLSRAT SEEQRFLVRL ITGELRQGAL EGVVLEAVAT ASGVPAADVR
160 170 180 190 200
RAFMLSGSLP DTATAALRGG AEALSAISLQ VGRPVRPMLA GPGSSLDAAL
210 220 230 240 250
ADLGPDVTVE FKLDGARVQV HRDGNQVRVW TRTLREVTDG VPELVERVRA
260 270 280 290 300
LPCRTAVLDG ETLALDDDGR PRAFQDTMSR FGSAGAGGEM PDAGVLLSPF
310 320 330 340 350
FFDLLHLDGR DLLDEPLDIR LDALAGLLAD DASAPLRMPG VRRPRPGQAA
360 370 380 390 400
QVLEHALDAG HEGVVVKDLA APYAAGRRGK AWQKVKPVHT LDLVVLGAEW
410 420 430 440 450
GYGRRTGTLS NIHLGARDPD GGEPVMVGKT FKGMTDELLA WQTRTFPEHT
460 470 480 490 500
REETPWGVLL EPALVVEIAV DGAQRSPRYP GGVALRFARV LRYRPDKTPA
510
EADTLEAVRA LLAGG
Length:515
Mass (Da):54,233
Last modified:April 18, 2012 - v1
Checksum:i1FBED4B2B38F3F32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO117623 Genomic DNA. Translation: CCG03749.1.
RefSeqiYP_005329780.1. NC_016943.1.

Genome annotation databases

EnsemblBacteriaiCCG03749; CCG03749; BLASA_2878.
GeneIDi11918476.
KEGGibsd:BLASA_2878.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO117623 Genomic DNA. Translation: CCG03749.1.
RefSeqiYP_005329780.1. NC_016943.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCG03749; CCG03749; BLASA_2878.
GeneIDi11918476.
KEGGibsd:BLASA_2878.

Phylogenomic databases

KOiK10747.
OMAiGRPRPFQ.

Enzyme and pathway databases

BioCyciBSAX1146883:GL9E-2786-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Blastococcus saxobsidens strain DD2."
    Genoscope.
    Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DD2Imported.

Entry informationi

Entry nameiH6RLM3_BLASD
AccessioniPrimary (citable) accession number: H6RLM3
Entry historyi
Integrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: March 4, 2015
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.