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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Nocardia cyriacigeorgica (strain GUH-2)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871Magnesium; catalyticUniRule annotation
Sitei88 – 881Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei111 – 1111Proton acceptorUniRule annotation
Binding sitei146 – 1461Substrate; shared with dimeric partnerUniRule annotation
Binding sitei206 – 2061SubstrateUniRule annotation
Binding sitei251 – 2511Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 572ATPUniRule annotation
Nucleotide bindingi86 – 894ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciNCYR1127134:GLHX-3964-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotationSAAS annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:NOCYR_3974Imported
OrganismiNocardia cyriacigeorgica (strain GUH-2)Imported
Taxonomic identifieri1127134 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
ProteomesiUP000008190 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi1127134.NOCYR_3974.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 1113Substrate bindingUniRule annotation
Regioni153 – 1553Substrate bindingUniRule annotation
Regioni257 – 2604Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiTVPRERY.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H6R716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRAVVRTAN GRYGDAIVGF QDGWRGLLED RKIQILNDDR TDRLLNKGGT
60 70 80 90 100
MLGTARTNPD VLREGLGRIQ QTLDDNGIEA LIPIGGEGTL TAASWLSDEG
110 120 130 140 150
VPVVGVPKTI DNDIDCTDVT FGHDTALAIA TEAIDRLHTT AESHQRVMLV
160 170 180 190 200
EVMGRHAGWI AIHAGMAAGA HLTLVPEVPF DVQQVCAMIK RRFQRGDKHF
210 220 230 240 250
ICVVAEGSHP APDSGFTLRE GGVDEFGHER FTGVAQQLGV EIERRIGKEV
260 270 280 290 300
RTTVLGHVQR GGSPTPYDRV LATRFGLHAA EAVHAGHFGQ MVALRGTEIG
310 320
LVPLSEATKQ LKRVPAERYR EAEAFFG
Length:327
Mass (Da):35,517
Last modified:April 18, 2012 - v1
Checksum:iA82B9FC05801D669
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO082843 Genomic DNA. Translation: CCF64735.1.
RefSeqiWP_014352187.1. NC_016887.1.

Genome annotation databases

EnsemblBacteriaiCCF64735; CCF64735; NOCYR_3974.
KEGGincy:NOCYR_3974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO082843 Genomic DNA. Translation: CCF64735.1.
RefSeqiWP_014352187.1. NC_016887.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1127134.NOCYR_3974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCF64735; CCF64735; NOCYR_3974.
KEGGincy:NOCYR_3974.

Phylogenomic databases

KOiK00850.
OMAiTVPRERY.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciNCYR1127134:GLHX-3964-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GUH-2Imported.

Entry informationi

Entry nameiH6R716_NOCCG
AccessioniPrimary (citable) accession number: H6R716
Entry historyi
Integrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: June 24, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.