H6Q3A1 (H6Q3A1_RICRI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281
EC=6.1.1.20 HAMAP-Rule MF_00281

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit HAMAP-Rule MF_00281

Gene names

Name:	pheS HAMAP-Rule MF_00281 EMBL AFB28892.1
ORF Names:	RPK_03225 EMBL AFB28892.1

Organism

Rickettsia rickettsii str. Hlp#2 EMBL AFB28892.1

Taxonomic identifier

1105101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia rickettsii

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281 SAAS SAAS004529
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00281 SAAS SAAS004529
Subunit structure	Tetramer of two alpha and two beta subunits By similarity. HAMAP-Rule MF_00281 SAAS SAAS004529
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00281 SAAS SAAS004529.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. HAMAP-Rule MF_00281

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00281 SAAS SAAS004529
Cellular component	Cytoplasm HAMAP-Rule MF_00281 SAAS SAAS004529
Ligand	ATP-binding HAMAP-Rule MF_00281 SAAS SAAS004529 Magnesium HAMAP-Rule MF_00281 SAAS SAAS004529 Metal-binding HAMAP-Rule MF_00281 SAAS SAAS004529 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00281 SAAS SAAS004529 EMBL AFB28892.1 Ligase
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	258	1	Magnesium By similarity HAMAP-Rule MF_00281

Sequences

	Sequence		Length	Mass (Da)
	H6Q3A1 [UniParc]. Last modified April 18, 2012. Version 1. Checksum: A1EEEB5A58EEEFD5	FASTA	349	40,320
	10 20 30 40 50 60 MENIGTILRL AEDKILLVQN LKALQEYKVE FLGKNGIVTG ELKKLGSLNE QERKEFGLKI 70 80 90 100 110 120 NKLKDKIQNI IKAKAEILEE QELNFKLAAD KIDLTIPARR YKQGSIHPIT QCSEELIQVF 130 140 150 160 170 180 SQFGFTIENG PNIENDFHNF TALNFEDDHP ARQMHDTFYL KSQENNKPLL LRTHTSTVQI 190 200 210 220 230 240 RAMKNGKPPF RFIAPGRTYR SDSDMTHTPM FHQIEGLVMD KNINMGHLKY VITTFIKSFF 250 260 270 280 290 300 ENSNIELRFR PSFFPFTEPS AEVDIRMNKN DKWLEVLGCG MVHPNVLKNV GIDSSEYQGF 310 320 330 340 AFGLGVERFA MLKYNIKDLR QFFEGDMRWL KHYNFGSFDI PNLAGGLTK

References

[1]	Johnson S.L., Davenport K.W., Han S., Bruce D.C., Dasch G.A. Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Hlp#2 EMBL AFB28892.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003311 Genomic DNA. Translation: AFB28892.1.
RefSeq	YP_005295312.1. NC_016915.1.
3D structure databases
ProteinModelPortal	H6Q3A1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AFB28892; AFB28892; RPK_03225.
GeneID	11864634.
KEGG	rrp:RPK_03225.
Phylogenomic databases
KO	K01889.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H6Q3A1_RICRI

Accession

Primary (citable) accession number: H6Q3A1

Entry history

Integrated into UniProtKB/TrEMBL:	April 18, 2012
Last sequence update:	April 18, 2012
Last modified:	April 3, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)