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H5T3E0 (H5T3E0_MELPD) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:MPD5_0408 EMBL BAL61683.1
OrganismMelissococcus plutonius (strain DAT561) [Complete proteome] [HAMAP] EMBL BAL61683.1
Taxonomic identifier1090974 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeMelissococcus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain136 – 341206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2951Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
H5T3E0 [UniParc].

Last modified April 18, 2012. Version 1.
Checksum: 4197403A4785345A

FASTA35640,335
        10         20         30         40         50         60 
MKIILLYGGK SEEHDVSILS AYSILNAIYY EYYTVQLVLI TKQGEWIKGP LLTEKPENKE 

        70         80         90        100        110        120 
ILNLTGAKDE NTMAYSSGTS IMPCEIFEKD AIVFPVLHGP NGEDGTIQGF LETIGMPYVG 

       130        140        150        160        170        180 
TGVLASATAM DKIMTKYLLQ TVGIPQTPYV PVLKNEWKND SKQLFERCEG TLLYPMFVKP 

       190        200        210        220        230        240 
ANLGSSVGIS KVENREELQK ALELAYKYDS RVIVEQGIEA REIEVAILGN EDVRTTLPGE 

       250        260        270        280        290        300 
VVKNVDFYDY QAKYVDNQIS MQIPAELPAE VCRQAQEYAK KAYKTLDGSG LSRCDFFLTN 

       310        320        330        340        350 
KNELFLNELN TMPGFTEFSM YPSLWENMGL KYSDLIEELI QLGLNRFEQR QRFFNN 

« Hide

References

[1]"Complete genome sequence of Melissococcus plutonius DAT561, a strain that shows an unusual growth profile and is representative of an endemic cluster in Japan."
Okumura K., Arai R., Okura M., Kirikae T., Takamatsu D., Osaki M., Miyoshi-Akiyama T.
J. Bacteriol. 194:3014-3014(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DAT561 EMBL BAL61683.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP012282 Genomic DNA. Translation: BAL61683.1.
RefSeqYP_005319178.1. NC_016938.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAL61683; BAL61683; MPD5_0408.
GeneID11878908.
KEGGmpx:MPD5_0408.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH5T3E0_MELPD
AccessionPrimary (citable) accession number: H5T3E0
Entry history
Integrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: February 19, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)