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Protein

Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial

Gene
N/A
Organism
Caenorhabditis japonica
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation
GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial, Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial, Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68Coenzyme AUniRule annotation1
Binding sitei185Substrate; shared with subunit betaUniRule annotation1
Active sitei277Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processTricarboxylic acid cycleUniRule annotation
LigandNucleotide-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrialUniRule annotation (EC:6.2.1.4UniRule annotation, EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit alphaUniRule annotation
Short name:
SCS-alphaUniRule annotation
OrganismiCaenorhabditis japonicaImported
Taxonomic identifieri281687 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000005237 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP-specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).UniRule annotation

Protein-protein interaction databases

STRINGi281687.CJA16822.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 125CoA_bindingInterPro annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 45Coenzyme A bindingUniRule annotation4
Regioni121 – 123Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1255. Eukaryota.
COG0074. LUCA.
InParanoidiH2WKC3.
OMAiIIFVPPA.
OrthoDBiEOG091G0D9C.

Family and domain databases

Gene3Di3.40.50.261. 1 hit.
HAMAPiMF_01988. Succ_CoA_alpha. 1 hit.
InterProiView protein in InterPro
IPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR033847. Citrt_syn/SCS-alpha_CS.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR036291. NAD(P)-bd_dom_sf.
IPR016102. Succinyl-CoA_synth-like.
PfamiView protein in Pfam
PF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
PIRSFiPIRSF001553. SucCS_alpha. 1 hit.
SMARTiView protein in SMART
SM00881. CoA_binding. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01019. sucCoAalpha. 1 hit.
PROSITEiView protein in PROSITE
PS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

H2WKC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTLASAAR SAARAVTRSV YSDTRNNLMI NRSTKVIIQG FTGRQGTFHG
60 70 80 90 100
KQMLEYNTQL VGGVSPNKAG QTHLGLPVFG SVAEAKDRTG ADATVIYVPA
110 120 130 140 150
SGAARAIHEA IDAEIGLIVA ITEGIPQQDM VRVKNRLLQQ NKSRLLGPNC
160 170 180 190 200
PGIIASGECK IGIMPGHIHK KGCIGIVSRS GTLTYEAVHQ TTAVGLGQTR
210 220 230 240 250
CIGIGGDPFN GTNFIDCLEV FLEDEKTKGI ILIGEIGGQA EEQAAEFLKS
260 270 280 290 300
RNSGPSAKPV VSFIAGVTAP PGRRMGHAGA IIAGGKGTAG DKIEALRQAN
310 320
VVVTDSPAKL GVAMQQALLG
Length:320
Mass (Da):33,273
Last modified:April 18, 2012 - v1
Checksum:i07748520086DFA13
GO

Genome annotation databases

EnsemblMetazoaiCJA16822; CJA16822; WBGene00136027.

Similar proteinsi

Entry informationi

Entry nameiH2WKC3_CAEJA
AccessioniPrimary (citable) accession number: H2WKC3
Entry historyiIntegrated into UniProtKB/TrEMBL: April 18, 2012
Last sequence update: April 18, 2012
Last modified: October 25, 2017
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported