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H2N0D4

- H2N0D4_ORYLA

UniProt

H2N0D4 - H2N0D4_ORYLA

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Protein

Alpha-amylase

Gene

LOC101163630

Organism
Oryzias latipes (Medaka fish) (Japanese ricefish)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151CalciumImported
Metal bindingi173 – 1731Calcium; via carbonyl oxygenImported
Metal bindingi182 – 1821CalciumImported
Metal bindingi216 – 2161Calcium; via carbonyl oxygenImported

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Name:LOC101163630Imported
OrganismiOryzias latipes (Medaka fish) (Japanese ricefish)Imported
Taxonomic identifieri8090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
ProteomesiUP000001038: Unplaced

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VM5X-ray2.85A16-512[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000002882.
InParanoidiH2N0D4.
KOiK01176.
OMAiSQVRDCR.
OrthoDBiEOG7RJPR2.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

H2N0D4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLFVLIALF GLGFAQHNPN TRDGRTAIVH LFEWRWADIA AECERFLGPK
60 70 80 90 100
GFAGVQISPP NEHILVSSPW RPWWQRYQPI SYNLCSRSGG ENELRDMITR
110 120 130 140 150
CNNVGVNVYV DAVINHMCGA GGGEGTHSSC GSWFNANNKD FPSVPYSNLD
160 170 180 190 200
FNDGKCKTGS GNIENYGDPY QVRDCRLVGL LDLALEKDYV RGKVADFMNK
210 220 230 240 250
LIDMGVAGFR VDACKHMWPG DLDNVYRRLN NLNTKWFPGG SRPFIFQEVI
260 270 280 290 300
DLGGEPITTG EYVGLGRVTE FKYGARLGEL FRKWNGQKLS YTKNWGEGWG
310 320 330 340 350
FMADGNAVVF TDNHDNQRGH GAGGASILTF WDPRLYKMAV GYMLAHPYGF
360 370 380 390 400
TRVMSSYSWD RNFVNGKDEN DWIGPPSNGD GSTKPVPINP DQTCGDGWVC
410 420 430 440 450
EHRWRQIMNM VQFRNVVNGQ PHANWWDNGN NQVAFGRGNR GFIVFNNDDW
460 470 480 490 500
ALDVTLNTGL PGGTYCDVIS GNKDGGSCTG KQITVGGDGR AHFYINNSEE
510
DPFIAIHADS KL
Length:512
Mass (Da):57,167
Last modified:March 21, 2012 - v1
Checksum:iFFDA8EFC8F527D02
GO

Sequence databases

RefSeqiXP_004085115.1. XM_004085067.1.

Genome annotation databases

EnsembliENSORLT00000024838; ENSORLP00000024837; ENSORLG00000020006.
GeneIDi101163630.
KEGGiola:101163630.

Cross-referencesi

Sequence databases

RefSeqi XP_004085115.1. XM_004085067.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VM5 X-ray 2.85 A 16-512 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSORLT00000024838 ; ENSORLP00000024837 ; ENSORLG00000020006 .
GeneIDi 101163630.
KEGGi ola:101163630.

Phylogenomic databases

GeneTreei ENSGT00390000002882.
InParanoidi H2N0D4.
KOi K01176.
OMAi SQVRDCR.
OrthoDBi EOG7RJPR2.
TreeFami TF312850.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hd-rRImported.
  2. "Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris."
    Mizutani K., Toyoda M., Otake Y., Yoshioka S., Takahashi N., Mikami B.
    Biochim. Biophys. Acta 1824:954-962(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 16-512 IN COMPLEX WITH CALCIUM.
  3. Ensembl
    Submitted (FEB-2012) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: Hd-rRImported.

Entry informationi

Entry nameiH2N0D4_ORYLA
AccessioniPrimary (citable) accession number: H2N0D4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: October 29, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteomeImported

External Data

Dasty 3