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Protein

Alpha-amylase

Gene

LOC101163630

Organism
Oryzias latipes (Japanese rice fish) (Japanese killifish)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumCombined sources1
Metal bindingi173Calcium; via carbonyl oxygenCombined sources1
Metal bindingi182CalciumCombined sources1
Metal bindingi216Calcium; via carbonyl oxygenCombined sources1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Name:LOC101163630Imported
OrganismiOryzias latipes (Japanese rice fish) (Japanese killifish)Imported
Taxonomic identifieri8090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
Proteomesi
  • UP000001038 Componenti: Unassembled WGS sequence

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 101Combined sources
Disulfide bondi85 ↔ 130Combined sources
Disulfide bondi156 ↔ 175Combined sources
Disulfide bondi394 ↔ 400Combined sources
Disulfide bondi466 ↔ 478Combined sources

Interactioni

Protein-protein interaction databases

STRINGi8090.ENSORLP00000024837.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VM5X-ray2.85A16-512[»]
SMRiH2N0D4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 414AamyInterPro annotationAdd BLAST389
Domaini423 – 511Aamy_CInterPro annotationAdd BLAST89

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
InParanoidiH2N0D4.
KOiK01176.
OMAiNWDTERD.
OrthoDBiEOG091G07V6.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

H2N0D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLFVLIALF GLGFAQHNPN TRDGRTAIVH LFEWRWADIA AECERFLGPK
60 70 80 90 100
GFAGVQISPP NEHILVSSPW RPWWQRYQPI SYNLCSRSGG ENELRDMITR
110 120 130 140 150
CNNVGVNVYV DAVINHMCGA GGGEGTHSSC GSWFNANNKD FPSVPYSNLD
160 170 180 190 200
FNDGKCKTGS GNIENYGDPY QVRDCRLVGL LDLALEKDYV RGKVADFMNK
210 220 230 240 250
LIDMGVAGFR VDACKHMWPG DLDNVYRRLN NLNTKWFPGG SRPFIFQEVI
260 270 280 290 300
DLGGEPITTG EYVGLGRVTE FKYGARLGEL FRKWNGQKLS YTKNWGEGWG
310 320 330 340 350
FMADGNAVVF TDNHDNQRGH GAGGASILTF WDPRLYKMAV GYMLAHPYGF
360 370 380 390 400
TRVMSSYSWD RNFVNGKDEN DWIGPPSNGD GSTKPVPINP DQTCGDGWVC
410 420 430 440 450
EHRWRQIMNM VQFRNVVNGQ PHANWWDNGN NQVAFGRGNR GFIVFNNDDW
460 470 480 490 500
ALDVTLNTGL PGGTYCDVIS GNKDGGSCTG KQITVGGDGR AHFYINNSEE
510
DPFIAIHADS KL
Length:512
Mass (Da):57,167
Last modified:March 21, 2012 - v1
Checksum:iFFDA8EFC8F527D02
GO

Sequence databases

RefSeqiXP_004085115.1. XM_004085067.2.

Genome annotation databases

EnsembliENSORLT00000024838; ENSORLP00000024837; ENSORLG00000020006.
GeneIDi101163630.
KEGGiola:101163630.

Cross-referencesi

Sequence databases

RefSeqiXP_004085115.1. XM_004085067.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VM5X-ray2.85A16-512[»]
SMRiH2N0D4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8090.ENSORLP00000024837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSORLT00000024838; ENSORLP00000024837; ENSORLG00000020006.
GeneIDi101163630.
KEGGiola:101163630.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
InParanoidiH2N0D4.
KOiK01176.
OMAiNWDTERD.
OrthoDBiEOG091G07V6.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiH2N0D4_ORYLA
AccessioniPrimary (citable) accession number: H2N0D4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: November 30, 2016
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.