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H2N0D4 (H2N0D4_ORYLA) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. RuleBase RU361134 SAAS SAAS006589

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. RuleBase RU003615

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. Ensembl ENSORLP00000024837

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding1151Calcium PDB 3VM5
Metal binding1731Calcium; via carbonyl oxygen PDB 3VM5
Metal binding1821Calcium PDB 3VM5
Metal binding2161Calcium; via carbonyl oxygen PDB 3VM5
Binding site2101Chloride PDB 3VM5
Binding site3521Chloride PDB 3VM5

Sequences

Sequence LengthMass (Da)Tools
H2N0D4 [UniParc].

Last modified March 21, 2012. Version 1.
Checksum: FFDA8EFC8F527D02

FASTA51257,167
        10         20         30         40         50         60 
MKLFVLIALF GLGFAQHNPN TRDGRTAIVH LFEWRWADIA AECERFLGPK GFAGVQISPP 

        70         80         90        100        110        120 
NEHILVSSPW RPWWQRYQPI SYNLCSRSGG ENELRDMITR CNNVGVNVYV DAVINHMCGA 

       130        140        150        160        170        180 
GGGEGTHSSC GSWFNANNKD FPSVPYSNLD FNDGKCKTGS GNIENYGDPY QVRDCRLVGL 

       190        200        210        220        230        240 
LDLALEKDYV RGKVADFMNK LIDMGVAGFR VDACKHMWPG DLDNVYRRLN NLNTKWFPGG 

       250        260        270        280        290        300 
SRPFIFQEVI DLGGEPITTG EYVGLGRVTE FKYGARLGEL FRKWNGQKLS YTKNWGEGWG 

       310        320        330        340        350        360 
FMADGNAVVF TDNHDNQRGH GAGGASILTF WDPRLYKMAV GYMLAHPYGF TRVMSSYSWD 

       370        380        390        400        410        420 
RNFVNGKDEN DWIGPPSNGD GSTKPVPINP DQTCGDGWVC EHRWRQIMNM VQFRNVVNGQ 

       430        440        450        460        470        480 
PHANWWDNGN NQVAFGRGNR GFIVFNNDDW ALDVTLNTGL PGGTYCDVIS GNKDGGSCTG 

       490        500        510 
KQITVGGDGR AHFYINNSEE DPFIAIHADS KL 

« Hide

References

« Hide 'large scale' references
[1]"The medaka draft genome and insights into vertebrate genome evolution."
Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T., Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A., Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A. expand/collapse author list , Asakawa S., Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S., Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K., Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.
Nature 447:714-719(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hd-rR Ensembl ENSORLP00000024837.
[2]"Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris."
Mizutani K., Toyoda M., Otake Y., Yoshioka S., Takahashi N., Mikami B.
Biochim. Biophys. Acta 1824:954-962(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 16-512 IN COMPLEX WITH CALCIUM AND CHLORIDE.
[3]Ensembl
Submitted (FEB-2012) to UniProtKB
Cited for: IDENTIFICATION.
Strain: Hd-rR Ensembl ENSORLP00000024837.

Cross-references

Sequence databases

RefSeqXP_004085115.1. XM_004085067.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VM5X-ray2.85A16-512[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSORLT00000024838; ENSORLP00000024837; ENSORLG00000020006.
GeneID101163630.
KEGGola:101163630.

Phylogenomic databases

GeneTreeENSGT00390000002882.
KOK01176.
OMASQVRDCR.
OrthoDBEOG7RJPR2.
TreeFamTF312850.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameH2N0D4_ORYLA
AccessionPrimary (citable) accession number: H2N0D4
Entry history
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: July 9, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)