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H2N0D4

- H2N0D4_ORYLA

UniProt

H2N0D4 - H2N0D4_ORYLA

Protein

Alpha-amylase

Gene

LOC101163630

Organism
Oryzias latipes (Medaka fish) (Japanese ricefish)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 18 (01 Oct 2014)
      Sequence version 1 (21 Mar 2012)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi115 – 1151CalciumImported
    Metal bindingi173 – 1731Calcium; via carbonyl oxygenImported
    Metal bindingi182 – 1821CalciumImported
    Metal bindingi216 – 2161Calcium; via carbonyl oxygenImported

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    GlycosidaseUniRule annotation, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolismUniRule annotation

    Keywords - Ligandi

    CalciumImported, Metal-bindingImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
    Gene namesi
    Name:LOC101163630Imported
    OrganismiOryzias latipes (Medaka fish) (Japanese ricefish)Imported
    Taxonomic identifieri8090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
    ProteomesiUP000001038: Unplaced

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VM5X-ray2.85A16-512[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.UniRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00390000002882.
    KOiK01176.
    OMAiSQVRDCR.
    OrthoDBiEOG7RJPR2.
    TreeFamiTF312850.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    H2N0D4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLFVLIALF GLGFAQHNPN TRDGRTAIVH LFEWRWADIA AECERFLGPK    50
    GFAGVQISPP NEHILVSSPW RPWWQRYQPI SYNLCSRSGG ENELRDMITR 100
    CNNVGVNVYV DAVINHMCGA GGGEGTHSSC GSWFNANNKD FPSVPYSNLD 150
    FNDGKCKTGS GNIENYGDPY QVRDCRLVGL LDLALEKDYV RGKVADFMNK 200
    LIDMGVAGFR VDACKHMWPG DLDNVYRRLN NLNTKWFPGG SRPFIFQEVI 250
    DLGGEPITTG EYVGLGRVTE FKYGARLGEL FRKWNGQKLS YTKNWGEGWG 300
    FMADGNAVVF TDNHDNQRGH GAGGASILTF WDPRLYKMAV GYMLAHPYGF 350
    TRVMSSYSWD RNFVNGKDEN DWIGPPSNGD GSTKPVPINP DQTCGDGWVC 400
    EHRWRQIMNM VQFRNVVNGQ PHANWWDNGN NQVAFGRGNR GFIVFNNDDW 450
    ALDVTLNTGL PGGTYCDVIS GNKDGGSCTG KQITVGGDGR AHFYINNSEE 500
    DPFIAIHADS KL 512
    Length:512
    Mass (Da):57,167
    Last modified:March 21, 2012 - v1
    Checksum:iFFDA8EFC8F527D02
    GO

    Sequence databases

    RefSeqiXP_004085115.1. XM_004085067.1.

    Genome annotation databases

    EnsembliENSORLT00000024838; ENSORLP00000024837; ENSORLG00000020006.
    GeneIDi101163630.
    KEGGiola:101163630.

    Cross-referencesi

    Sequence databases

    RefSeqi XP_004085115.1. XM_004085067.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VM5 X-ray 2.85 A 16-512 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSORLT00000024838 ; ENSORLP00000024837 ; ENSORLG00000020006 .
    GeneIDi 101163630.
    KEGGi ola:101163630.

    Phylogenomic databases

    GeneTreei ENSGT00390000002882.
    KOi K01176.
    OMAi SQVRDCR.
    OrthoDBi EOG7RJPR2.
    TreeFami TF312850.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hd-rRImported.
    2. "Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris."
      Mizutani K., Toyoda M., Otake Y., Yoshioka S., Takahashi N., Mikami B.
      Biochim. Biophys. Acta 1824:954-962(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 16-512 IN COMPLEX WITH CALCIUM.
    3. Ensembl
      Submitted (FEB-2012) to UniProtKB
      Cited for: IDENTIFICATION.
      Strain: Hd-rRImported.

    Entry informationi

    Entry nameiH2N0D4_ORYLA
    AccessioniPrimary (citable) accession number: H2N0D4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 21, 2012
    Last sequence update: March 21, 2012
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Caution

    The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3