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H2N0D4

- H2N0D4_ORYLA

UniProt

H2N0D4 - H2N0D4_ORYLA

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Protein

Alpha-amylase

Gene
LOC101163630
Organism
Oryzias latipes (Medaka fish) (Japanese ricefish)
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151CalciumImported
Metal bindingi173 – 1731Calcium; via carbonyl oxygenImported
Metal bindingi182 – 1821CalciumImported
Metal bindingi216 – 2161Calcium; via carbonyl oxygenImported

GO - Molecular functioni

  1. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Name:LOC101163630Imported
OrganismiOryzias latipes (Medaka fish) (Japanese ricefish)Imported
Taxonomic identifieri8090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
ProteomesiUP000001038: Unplaced

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VM5X-ray2.85A16-512[»]

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000002882.
KOiK01176.
OMAiSQVRDCR.
OrthoDBiEOG7RJPR2.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

H2N0D4-1 [UniParc]FASTAAdd to Basket

« Hide

MKLFVLIALF GLGFAQHNPN TRDGRTAIVH LFEWRWADIA AECERFLGPK    50
GFAGVQISPP NEHILVSSPW RPWWQRYQPI SYNLCSRSGG ENELRDMITR 100
CNNVGVNVYV DAVINHMCGA GGGEGTHSSC GSWFNANNKD FPSVPYSNLD 150
FNDGKCKTGS GNIENYGDPY QVRDCRLVGL LDLALEKDYV RGKVADFMNK 200
LIDMGVAGFR VDACKHMWPG DLDNVYRRLN NLNTKWFPGG SRPFIFQEVI 250
DLGGEPITTG EYVGLGRVTE FKYGARLGEL FRKWNGQKLS YTKNWGEGWG 300
FMADGNAVVF TDNHDNQRGH GAGGASILTF WDPRLYKMAV GYMLAHPYGF 350
TRVMSSYSWD RNFVNGKDEN DWIGPPSNGD GSTKPVPINP DQTCGDGWVC 400
EHRWRQIMNM VQFRNVVNGQ PHANWWDNGN NQVAFGRGNR GFIVFNNDDW 450
ALDVTLNTGL PGGTYCDVIS GNKDGGSCTG KQITVGGDGR AHFYINNSEE 500
DPFIAIHADS KL 512
Length:512
Mass (Da):57,167
Last modified:March 21, 2012 - v1
Checksum:iFFDA8EFC8F527D02
GO

Sequence databases

RefSeqiXP_004085115.1. XM_004085067.1.

Genome annotation databases

EnsembliENSORLT00000024838; ENSORLP00000024837; ENSORLG00000020006.
GeneIDi101163630.
KEGGiola:101163630.

Cross-referencesi

Sequence databases

RefSeqi XP_004085115.1. XM_004085067.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VM5 X-ray 2.85 A 16-512 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSORLT00000024838 ; ENSORLP00000024837 ; ENSORLG00000020006 .
GeneIDi 101163630.
KEGGi ola:101163630.

Phylogenomic databases

GeneTreei ENSGT00390000002882.
KOi K01176.
OMAi SQVRDCR.
OrthoDBi EOG7RJPR2.
TreeFami TF312850.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hd-rRImported.
  2. "Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris."
    Mizutani K., Toyoda M., Otake Y., Yoshioka S., Takahashi N., Mikami B.
    Biochim. Biophys. Acta 1824:954-962(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 16-512 IN COMPLEX WITH CALCIUM.
  3. Ensembl
    Submitted (FEB-2012) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: Hd-rRImported.

Entry informationi

Entry nameiH2N0D4_ORYLA
AccessioniPrimary (citable) accession number: H2N0D4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: September 3, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteome

External Data

Dasty 3

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