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Protein

Glycogenin-1

Gene

gyg-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for gsy-1.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.1 Publication

Cofactori

Mn2+By similarityNote: Divalent metal ions. Required for self-glucosylation. Manganese is the most effective.By similarity

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85By similarity1
Metal bindingi101ManganeseBy similarity1
Metal bindingi103ManganeseBy similarity1
Sitei159Important for catalytic activityBy similarity1
Sitei162Important for catalytic activityBy similarity1
Metal bindingi211ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlycogen biosynthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-3322077. Glycogen synthesis.
R-CEL-6798695. Neutrophil degranulation.
R-CEL-70221. Glycogen breakdown (glycogenolysis).
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogenin-1Imported (EC:2.4.1.186By similarity)
Short name:
GN1 Publication
Gene namesi
Name:gyg-1Imported
ORF Names:F56B6.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF56B6.4a; CE04664; WBGene00006863; gyg-1.
F56B6.4b; CE29405; WBGene00006863; gyg-1.
F56B6.4c; CE31185; WBGene00006863; gyg-1.

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi194Y → P: Loss of auto-glucosylation. No effect on interaction with gsy-1 but loss of gsy-1 function. 1 Publication1
Mutagenesisi400R → A: Loss of interaction with gsy-1. Partial loss of gsy-1 function. 1 Publication1
Mutagenesisi403W → A: Loss of interaction with gsy-1. Partial loss of gsy-1 function. 1 Publication1
Mutagenesisi410Y → A: Loss of interaction with gsy-1. Partial loss of gsy-1 function. 1 Publication1
Mutagenesisi416F → A: Loss of interaction with gsy-1. Complete loss of gsy-1 function. 1 Publication1
Mutagenesisi419I → A: Loss of interaction with gsy-1. Complete loss of gsy-1 function. 1 Publication1
Mutagenesisi423L → A: Loss of interaction with gsy-1. Complete loss of gsy-1 function. 1 Publication1
Mutagenesisi427L → A: Loss of interaction with gsy-1. Partial loss of gsy-1 function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004315091 – 429Glycogenin-1CuratedAdd BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi194O-linked (Glc...) tyrosine1 Publication1

Post-translational modificationi

Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-194.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiH2KYQ5.
PaxDbiH2KYQ5.

Expressioni

Gene expression databases

BgeeiWBGene00006863.

Interactioni

Subunit structurei

Forms a heterooctamer with one molecule of gyg-1 bound to each protomer of the gys-1 homotetramer. The N-terminus of gys-1 is involved in interprotomer contacts with gyg-1. The interaction with gys-1 is required for glycogen production but is not required for gys-1 intrinsic activity.1 Publication

Protein-protein interaction databases

IntActiH2KYQ5. 1 interactor.
STRINGi6239.F56B6.4a.1.

Structurei

Secondary structure

1429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi396 – 404Combined sources9
Turni410 – 414Combined sources5
Helixi416 – 426Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QLBX-ray2.60E/F/G/H394-429[»]
ProteinModelPortaliH2KYQ5.
SMRiH2KYQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The length of the linker region between residues 387-394 which separates two alpha-helices regulates the size of the glycogen particles synthesized but plays no role in the interaction with gsy-1.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1950. Eukaryota.
COG5597. LUCA.
GeneTreeiENSGT00390000004721.
HOGENOMiHOG000008282.
KOiK00750.
OMAiWPDSFNS.
OrthoDBiEOG091G09E1.
PhylomeDBiH2KYQ5.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF01501. Glyco_transf_8. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: H2KYQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEAWITLAT NDNYAQGALV LVHSLRTAGT TRKIHCLISN EVSAPVRKQL
60 70 80 90 100
EEHFDDVSIV DVFNSNDSDN LRLIERPDLG VTFTKLHCWR LTQYTKCVFL
110 120 130 140 150
DADTLVLRNA DELFTRPDFS AASDIGWPDS FNSGVFVYVP NNETYRQLVD
160 170 180 190 200
FAVTHGSYDG GDQGLLNDFF SNWRDLPSEH RLPFIYNMTA GAFYTYAAAY
210 220 230 240 250
KRYGANTKIV HFIGSVKPWH GSAAVHTGEH FQQWQKIYHA HVNHTSRTNE
260 270 280 290 300
HAAVFPSHHH TPEHRSHSAD HPKIERKDSI VREIGNFVMH VVQSVNILPS
310 320 330 340 350
YDTDANTSDS HRNNEPHKHD QQREEHHELP HNKFQTPHEE SQDIVGSTDC
360 370 380 390 400
FGSQMPKYNA DSETDREVEQ ITNNTPCPAF VPFERREEYQ AASPSTEERR
410 420
AAWEAGQPDY LGRDAFVHIQ EALNRALNE
Note: No experimental confirmation available.Curated
Length:429
Mass (Da):49,007
Last modified:March 21, 2012 - v1
Checksum:iAD3856F797662654
GO
Isoform bImported (identifier: H2KYQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-390: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:284
Mass (Da):32,174
Checksum:i1759DF990420DD9C
GO
Isoform cImported (identifier: H2KYQ5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-371: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:303
Mass (Da):34,454
Checksum:i8191A7BFC6444DF3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057309246 – 390Missing in isoform b. CuratedAdd BLAST145
Alternative sequenceiVSP_057310246 – 371Missing in isoform c. CuratedAdd BLAST126

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080515 Genomic DNA. Translation: CCD64318.1.
FO080515 Genomic DNA. Translation: CCD64319.1.
FO080515 Genomic DNA. Translation: CCD64320.1.
RefSeqiNP_508608.1. NM_076207.5. [H2KYQ5-1]
NP_508609.1. NM_076208.6. [H2KYQ5-2]
NP_741749.1. NM_171944.4. [H2KYQ5-3]
UniGeneiCel.6394.

Genome annotation databases

EnsemblMetazoaiF56B6.4a; F56B6.4a; WBGene00006863. [H2KYQ5-1]
GeneIDi180644.
KEGGicel:CELE_F56B6.4.
UCSCiF56B6.4c. c. elegans.

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiGYG1_CAEEL
AccessioniPrimary (citable) accession number: H2KYQ5
Secondary accession number(s): H2KYQ6, Q95Q50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: March 21, 2012
Last modified: September 27, 2017
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families