H2KWF1 (PAKSY_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Parkeol synthase
EC=5.4.99.47

Gene names

Ordered Locus Names:	Os11g0189600, LOC_Os11g08569
ORF Names:	OsJ_33246

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically mediates the conversion of oxidosqualene ((3S)-2,3-epoxy-2,3-dihydrosqualene) to parkeol. Ref.6
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = parkeol. Ref.6
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 4 PFTB repeats.
Sequence caution	The sequence AAX95229.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes. The sequence AAX95231.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes. The sequence ABG22398.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAG89914.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAH95124.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAZ17703.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	intramolecular transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Parkeol synthase		PRO_0000418965
Regions
	Transmembrane	118 – 138	21	Helical; Potential
	Repeat	147 – 188	42	PFTB 1
	Repeat	512 – 557	46	PFTB 2
	Repeat	589 – 629	41	PFTB 3
	Repeat	638 – 689	52	PFTB 4
Sites
	Active site	483	1	Proton donor Potential

Sequences

	Sequence		Length	Mass (Da)
	H2KWF1 [UniParc]. Last modified March 21, 2012. Version 1. Checksum: 98EFB2E0088C5675	FASTA	759	86,127
	10 20 30 40 50 60 MWRLKVSEGG SPWLRSVNNL LGRQVWEFDP DLGTPEERAD VEKARREFAE HRFERKHSSD 70 80 90 100 110 120 LLMRMQFAKE NCQKLDLLAV KRGEHEDVMG EAVWSSLKRA ISRVCNLQAH DGHWPGDYAG 130 140 150 160 170 180 LMFFLPGLII TLHVSGVLNT VLSSEHQKEM RRYIYNHQNE DGGWGLHIEG HSTMLGSSLN 190 200 210 220 230 240 YVALRLLGEG PNGGDGCIEN GRNWILDHGG ATFTTSWGKF WLSVLGVFDW SGNNPVPPEL 250 260 270 280 290 300 LLLPYQLPFH PGRMSSYIRM VFIPMSYIYG KRFVGPVTPV VLELRSELYN DPYDEIDWNK 310 320 330 340 350 360 ARTQCAKEDM YYPRSSKLDM FWSFLHKFIE PVLLRWPGRK LREKALATSM RNVHYEDECT 370 380 390 400 410 420 RYICFGGVPK ALNILACWIE DPSSEAFKCH IARVYDYLWI AEDGMKMQIY DGSQVWDAGL 430 440 450 460 470 480 TVEALVATDL VKELGPTLKR AHSFLKNSQL LDNCPRDFNR WYRHISKGGW TFTTADDGWQ 490 500 510 520 530 540 VSDCTATALK ACLLLSRISP EIVGEPLEID AQYDAVNCLM SLMNDNGGFS AFELVRSNTW 550 560 570 580 590 600 LEHINPTEAF GRVMIEYPYV ECTSSSIQCL ALFKKLHPGH RKEEVENCIS KGANFIESSQ 610 620 630 640 650 660 RSDGSWYGSW GICFTYATWF AVTGLVSAGR TLGNSATVRK ACDFLLSKQL PSGGWGESYL 670 680 690 700 710 720 SCHDEVYTNL KGNRPHGTHT AWAMIALIDA GQAERDPVPL HRAAKALLNL QLEDGEFPQQ 730 740 750 EIVGVFLQTA MISYSQYRNI FPIMALTGYR RRVLLAGNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and achilleol B synthases." Ito R., Mori K., Hashimoto I., Nakano C., Sato T., Hoshino T. Org. Lett. 13:2678-2681(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC134046 Genomic DNA. Translation: AAX95229.1. Sequence problems. AC134046 Genomic DNA. Translation: AAX95231.1. Sequence problems. DP000010 Genomic DNA. Translation: ABG22399.1. DP000010 Genomic DNA. Translation: ABG22398.1. Sequence problems. AP008217 Genomic DNA. Translation: BAH95124.1. Sequence problems. CM000148 Genomic DNA. Translation: EAZ17703.1. Sequence problems. AK066327 mRNA. Translation: BAG89914.1. Different initiation.
RefSeq	NP_001176396.1. NM_001189467.1.
UniGene	Os.87784.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os11g08569.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9268610.
KEGG	osa:9268610.
Phylogenomic databases
OMA	EEVENCI.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAKSY_ORYSJ

Accession

Primary (citable) accession number: H2KWF1
Secondary accession number(s): B7EC17 , C7J910, Q53NJ6, Q53NK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	March 21, 2012
Last modified:	April 3, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program