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H2KWF1

- PAKSY_ORYSJ

UniProt

H2KWF1 - PAKSY_ORYSJ

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Protein

Parkeol synthase

Gene

Os11g0189600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Specifically mediates the conversion of oxidosqualene ((3S)-2,3-epoxy-2,3-dihydrosqualene) to parkeol.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = parkeol.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei483 – 4831Proton donorSequence Analysis

GO - Molecular functioni

  1. intramolecular transferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17969.
ReactomeiREACT_255488. Cholesterol biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Parkeol synthase (EC:5.4.99.47)
Gene namesi
Ordered Locus Names:Os11g0189600, LOC_Os11g08569
ORF Names:OsJ_33246
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 11

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Parkeol synthasePRO_0000418965Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os11g08569.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati147 – 18842PFTB 1Add
BLAST
Repeati512 – 55746PFTB 2Add
BLAST
Repeati589 – 62941PFTB 3Add
BLAST
Repeati638 – 68952PFTB 4Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiH2KWF1.
OMAiCHIARVY.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H2KWF1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWRLKVSEGG SPWLRSVNNL LGRQVWEFDP DLGTPEERAD VEKARREFAE
60 70 80 90 100
HRFERKHSSD LLMRMQFAKE NCQKLDLLAV KRGEHEDVMG EAVWSSLKRA
110 120 130 140 150
ISRVCNLQAH DGHWPGDYAG LMFFLPGLII TLHVSGVLNT VLSSEHQKEM
160 170 180 190 200
RRYIYNHQNE DGGWGLHIEG HSTMLGSSLN YVALRLLGEG PNGGDGCIEN
210 220 230 240 250
GRNWILDHGG ATFTTSWGKF WLSVLGVFDW SGNNPVPPEL LLLPYQLPFH
260 270 280 290 300
PGRMSSYIRM VFIPMSYIYG KRFVGPVTPV VLELRSELYN DPYDEIDWNK
310 320 330 340 350
ARTQCAKEDM YYPRSSKLDM FWSFLHKFIE PVLLRWPGRK LREKALATSM
360 370 380 390 400
RNVHYEDECT RYICFGGVPK ALNILACWIE DPSSEAFKCH IARVYDYLWI
410 420 430 440 450
AEDGMKMQIY DGSQVWDAGL TVEALVATDL VKELGPTLKR AHSFLKNSQL
460 470 480 490 500
LDNCPRDFNR WYRHISKGGW TFTTADDGWQ VSDCTATALK ACLLLSRISP
510 520 530 540 550
EIVGEPLEID AQYDAVNCLM SLMNDNGGFS AFELVRSNTW LEHINPTEAF
560 570 580 590 600
GRVMIEYPYV ECTSSSIQCL ALFKKLHPGH RKEEVENCIS KGANFIESSQ
610 620 630 640 650
RSDGSWYGSW GICFTYATWF AVTGLVSAGR TLGNSATVRK ACDFLLSKQL
660 670 680 690 700
PSGGWGESYL SCHDEVYTNL KGNRPHGTHT AWAMIALIDA GQAERDPVPL
710 720 730 740 750
HRAAKALLNL QLEDGEFPQQ EIVGVFLQTA MISYSQYRNI FPIMALTGYR

RRVLLAGNI
Length:759
Mass (Da):86,127
Last modified:March 21, 2012 - v1
Checksum:i98EFB2E0088C5675
GO

Sequence cautioni

The sequence AAX95229.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.Curated
The sequence AAX95231.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.Curated
The sequence ABG22398.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAG89914.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAH95124.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAZ17703.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC134046 Genomic DNA. Translation: AAX95229.1. Sequence problems.
AC134046 Genomic DNA. Translation: AAX95231.1. Sequence problems.
DP000010 Genomic DNA. Translation: ABG22399.1.
DP000010 Genomic DNA. Translation: ABG22398.1. Sequence problems.
AP008217 Genomic DNA. Translation: BAH95124.1. Sequence problems.
CM000148 Genomic DNA. Translation: EAZ17703.1. Sequence problems.
AK066327 mRNA. Translation: BAG89914.1. Different initiation.
RefSeqiNP_001176396.1. NM_001189467.1.
UniGeneiOs.87784.

Genome annotation databases

EnsemblPlantsiOS11T0189600-01; OS11T0189600-01; OS11G0189600.
GeneIDi9268610.
KEGGiosa:9268610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC134046 Genomic DNA. Translation: AAX95229.1 . Sequence problems.
AC134046 Genomic DNA. Translation: AAX95231.1 . Sequence problems.
DP000010 Genomic DNA. Translation: ABG22399.1 .
DP000010 Genomic DNA. Translation: ABG22398.1 . Sequence problems.
AP008217 Genomic DNA. Translation: BAH95124.1 . Sequence problems.
CM000148 Genomic DNA. Translation: EAZ17703.1 . Sequence problems.
AK066327 mRNA. Translation: BAG89914.1 . Different initiation.
RefSeqi NP_001176396.1. NM_001189467.1.
UniGenei Os.87784.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os11g08569.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS11T0189600-01 ; OS11T0189600-01 ; OS11G0189600 .
GeneIDi 9268610.
KEGGi osa:9268610.

Phylogenomic databases

InParanoidi H2KWF1.
OMAi CHIARVY.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17969.
Reactomei REACT_255488. Cholesterol biosynthesis.

Family and domain databases

Gene3Di 1.50.10.20. 2 hits.
InterProi IPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00432. Prenyltrans. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 2 hits.
TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
PROSITEi PS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and achilleol B synthases."
    Ito R., Mori K., Hashimoto I., Nakano C., Sato T., Hoshino T.
    Org. Lett. 13:2678-2681(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPAKSY_ORYSJ
AccessioniPrimary (citable) accession number: H2KWF1
Secondary accession number(s): B7EC17
, C7J910, Q53NJ6, Q53NK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 21, 2012
Last modified: November 26, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3