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H2KWF1 (PAKSY_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Parkeol synthase

EC=5.4.99.47
Gene names
Ordered Locus Names:Os11g0189600, LOC_Os11g08569
ORF Names:OsJ_33246
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically mediates the conversion of oxidosqualene ((3S)-2,3-epoxy-2,3-dihydrosqualene) to parkeol. Ref.6

Catalytic activity

(3S)-2,3-epoxy-2,3-dihydrosqualene = parkeol. Ref.6

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 4 PFTB repeats.

Sequence caution

The sequence AAX95229.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.

The sequence AAX95231.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.

The sequence ABG22398.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAG89914.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAH95124.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAZ17703.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentMembrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionintramolecular transferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759Parkeol synthase
PRO_0000418965

Regions

Transmembrane118 – 13821Helical; Potential
Repeat147 – 18842PFTB 1
Repeat512 – 55746PFTB 2
Repeat589 – 62941PFTB 3
Repeat638 – 68952PFTB 4

Sites

Active site4831Proton donor Potential

Sequences

Sequence LengthMass (Da)Tools
H2KWF1 [UniParc].

Last modified March 21, 2012. Version 1.
Checksum: 98EFB2E0088C5675

FASTA75986,127
        10         20         30         40         50         60 
MWRLKVSEGG SPWLRSVNNL LGRQVWEFDP DLGTPEERAD VEKARREFAE HRFERKHSSD 

        70         80         90        100        110        120 
LLMRMQFAKE NCQKLDLLAV KRGEHEDVMG EAVWSSLKRA ISRVCNLQAH DGHWPGDYAG 

       130        140        150        160        170        180 
LMFFLPGLII TLHVSGVLNT VLSSEHQKEM RRYIYNHQNE DGGWGLHIEG HSTMLGSSLN 

       190        200        210        220        230        240 
YVALRLLGEG PNGGDGCIEN GRNWILDHGG ATFTTSWGKF WLSVLGVFDW SGNNPVPPEL 

       250        260        270        280        290        300 
LLLPYQLPFH PGRMSSYIRM VFIPMSYIYG KRFVGPVTPV VLELRSELYN DPYDEIDWNK 

       310        320        330        340        350        360 
ARTQCAKEDM YYPRSSKLDM FWSFLHKFIE PVLLRWPGRK LREKALATSM RNVHYEDECT 

       370        380        390        400        410        420 
RYICFGGVPK ALNILACWIE DPSSEAFKCH IARVYDYLWI AEDGMKMQIY DGSQVWDAGL 

       430        440        450        460        470        480 
TVEALVATDL VKELGPTLKR AHSFLKNSQL LDNCPRDFNR WYRHISKGGW TFTTADDGWQ 

       490        500        510        520        530        540 
VSDCTATALK ACLLLSRISP EIVGEPLEID AQYDAVNCLM SLMNDNGGFS AFELVRSNTW 

       550        560        570        580        590        600 
LEHINPTEAF GRVMIEYPYV ECTSSSIQCL ALFKKLHPGH RKEEVENCIS KGANFIESSQ 

       610        620        630        640        650        660 
RSDGSWYGSW GICFTYATWF AVTGLVSAGR TLGNSATVRK ACDFLLSKQL PSGGWGESYL 

       670        680        690        700        710        720 
SCHDEVYTNL KGNRPHGTHT AWAMIALIDA GQAERDPVPL HRAAKALLNL QLEDGEFPQQ 

       730        740        750 
EIVGVFLQTA MISYSQYRNI FPIMALTGYR RRVLLAGNI 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
The rice chromosomes 11 and 12 sequencing consortia
BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[6]"Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and achilleol B synthases."
Ito R., Mori K., Hashimoto I., Nakano C., Sato T., Hoshino T.
Org. Lett. 13:2678-2681(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC134046 Genomic DNA. Translation: AAX95229.1. Sequence problems.
AC134046 Genomic DNA. Translation: AAX95231.1. Sequence problems.
DP000010 Genomic DNA. Translation: ABG22399.1.
DP000010 Genomic DNA. Translation: ABG22398.1. Sequence problems.
AP008217 Genomic DNA. Translation: BAH95124.1. Sequence problems.
CM000148 Genomic DNA. Translation: EAZ17703.1. Sequence problems.
AK066327 mRNA. Translation: BAG89914.1. Different initiation.
RefSeqNP_001176396.1. NM_001189467.1.
UniGeneOs.87784.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os11g08569.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS11T0189600-01; OS11T0189600-01; OS11G0189600.
GeneID9268610.
KEGGosa:9268610.

Phylogenomic databases

OMACHIARVY.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17969.

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01787. squalene_cyclas. 1 hit.
PROSITEPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePAKSY_ORYSJ
AccessionPrimary (citable) accession number: H2KWF1
Secondary accession number(s): B7EC17 expand/collapse secondary AC list , C7J910, Q53NJ6, Q53NK1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 21, 2012
Last modified: April 16, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations