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H2IGJ5 (H2IGJ5_9VIBR) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897
Gene names
Name:gyrA HAMAP-Rule MF_01897
ORF Names:VEJY3_10125 EMBL AEX22505.1
OrganismVibrio sp. EJY3 EMBL AEX22505.1
Taxonomic identifier1116375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1221O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

Sequence LengthMass (Da)Tools
H2IGJ5 [UniParc].

Last modified March 21, 2012. Version 1.
Checksum: 552BDC1584FAAC59

FASTA87796,887
        10         20         30         40         50         60 
MSDLAKEITP VNIEDELRGS YLDYAMSVIV GRALPDVRDG LKPVHRRVLF AMNVLGNDWN 

        70         80         90        100        110        120 
KPYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM 

       130        140        150        160        170        180 
RYTEVRMAKI AHELLADLDK ETVDYVPNYD GTEQIPAVLP TKIPNLLVNG ASGIAVGMAT 

       190        200        210        220        230        240 
NIPPHNLGEV IDGCLAYINN EAITIDELMD YIPGPDFPTA ALISGRKGIV DAYKTGRGKI 

       250        260        270        280        290        300 
YMRSKADIET EKNGKETIIV TEIPYQVNKA RLIEKIAELV KDKKVEGISA LRDESDKDGM 

       310        320        330        340        350        360 
RIVIECKRDA VGEVVLNNLY AQTQLQTTFG INMVALNNGQ PQLFNLKDML KCFVDHRREV 

       370        380        390        400        410        420 
VTRRTIFELR KARERAHILE GLALALANID EIIDLIRKAP TPAEAKAGLI ARGWDLGNVA 

       430        440        450        460        470        480 
AMLERAGTDA ARPEWLEPQY GIRDGQYFLT EQQAQAILDL RLHKLTGLEH EKILAEYKEL 

       490        500        510        520        530        540 
LDEIAELMHI LASTERLMEV IREELEAVRD GFADERRTEI TAASHDIDME ELIAREDVVV 

       550        560        570        580        590        600 
TLSHGGYVKY QVLSDYESQR RGGKGKSATK MKDEDHIERL LVANTHDNIL CFSTRGKTYR 

       610        620        630        640        650        660 
LKVYQLPLAS RTARGKPIVN ILPLEDGERI TAILRVDEFS EDKFIFMATG DGTVKKTPLN 

       670        680        690        700        710        720 
QFANVRSNGL IAVNLRDDDS LIGVDITNGD SDIMLFSKAG KVVRFSEDKV RAMGRTAAGV 

       730        740        750        760        770        780 
RGMKLGEDDQ VVSLIVPSNE GDILTVTENG YGKRTELAEY PTKGRATQGV VSIKVSERNG 

       790        800        810        820        830        840 
PVVGAVQVEE GDEMMMITDA GTLVRTRVAE VSQVGRNTQG VTLIRTAEDE TVVGLQRIDE 

       850        860        870 
IEDADLPEGE EGESTEPSAE QQAPEAPESP AAEDSED

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References

[1]"Genome Sequence of Vibrio sp. Strain EJY3, an Agarolytic Marine Bacterium Metabolizing 3,6-Anhydro-L-Galactose as a Sole Carbon Source."
Roh H., Yun E.J., Lee S., Ko H.J., Kim S., Kim B.Y., Song H., Lim K.I., Kim K.H., Choi I.G.
J. Bacteriol. 194:2773-2774(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: EJY3 EMBL AEX22505.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003241 Genomic DNA. Translation: AEX22505.1.
RefSeqYP_005023483.1. NC_016613.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEX22505; AEX22505; VEJY3_10125.
GeneID11667068.
KEGGvej:VEJY3_10125.

Phylogenomic databases

KOK02469.

Family and domain databases

Gene3D1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.90.199.10. 1 hit.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameH2IGJ5_9VIBR
AccessionPrimary (citable) accession number: H2IGJ5
Entry history
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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