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H2ID69 (H2ID69_9VIBR) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
EC=2.1.1.182 HAMAP-Rule MF_00607
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase HAMAP-Rule MF_00607
16S rRNA dimethyladenosine transferase HAMAP-Rule MF_00607
16S rRNA dimethylase HAMAP-Rule MF_00607
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase HAMAP-Rule MF_00607
Gene names
Name:ksgA HAMAP-Rule MF_00607
Synonyms:rsmA HAMAP-Rule MF_00607
ORF Names:VEJY3_01645 EMBL AEX20827.1
OrganismVibrio sp. EJY3 EMBL AEX20827.1
Taxonomic identifier1116375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607 SAAS SAAS023165

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00607 SAAS SAAS023165.

Sequence similarities

Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily. HAMAP-Rule MF_00607

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site181S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site201S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP-Rule MF_00607
Binding site451S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site661S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site911S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site1121S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607

Sequences

Sequence LengthMass (Da)Tools
H2ID69 [UniParc].

Last modified March 21, 2012. Version 1.
Checksum: AFB944BB379DA6C6

FASTA26930,633
        10         20         30         40         50         60 
MRNDVHLGHK ARKRFGQNFL NDPYIIDGIV SAINPRPGQN LVEIGPGLGA ITEPVGREVD 

        70         80         90        100        110        120 
KFTVIELDRD LAERLRNHPE LADKLTIHEG DAMRFDFTQL VKPNNKLRIF GNLPYNISTP 

       130        140        150        160        170        180 
LMFHLFEFHK DIQDMHFMLQ KEVVNRLAAG PGSKAYGRLT VMAQYYCKVV PVLEVPPTAF 

       190        200        210        220        230        240 
VPPPKVDSAV VRLVPYEVLP CPAKDLRLLD RVCREGFNQR RKTVRNCYKS LLSTEVLEEL 

       250        260 
GINPSMRPEN LTLEQFVAMA NWLADNPQH

« Hide

References

[1]"Genome Sequence of Vibrio sp. Strain EJY3, an Agarolytic Marine Bacterium Metabolizing 3,6-Anhydro-L-Galactose as a Sole Carbon Source."
Roh H., Yun E.J., Lee S., Ko H.J., Kim S., Kim B.Y., Song H., Lim K.I., Kim K.H., Choi I.G.
J. Bacteriol. 194:2773-2774(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: EJY3 EMBL AEX20827.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003241 Genomic DNA. Translation: AEX20827.1.
RefSeqYP_005021802.1. NC_016613.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEX20827; AEX20827; VEJY3_01645.
GeneID11665988.
KEGGvej:VEJY3_01645.

Phylogenomic databases

KOK02528.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH2ID69_9VIBR
AccessionPrimary (citable) accession number: H2ID69
Entry history
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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