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H2I9E9 (H2I9E9_CORDV) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283
EC=6.1.1.20 HAMAP-Rule MF_00283
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit HAMAP-Rule MF_00283
Gene names
Name:pheT HAMAP-Rule MF_00283 EMBL AEX83307.1
Ordered Locus Names:CDVA01_1038 EMBL AEX83307.1
OrganismCorynebacterium diphtheriae (strain VA01) [Complete proteome] [HAMAP] EMBL AEX83307.1
Taxonomic identifier698971 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium ›

Protein attributes

Sequence length836 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). SAAS SAAS002547 HAMAP-Rule MF_00283

Cofactor

Binds 2 magnesium ions per tetramer By similarity. SAAS SAAS002547 HAMAP-Rule MF_00283

Subunit structure

Tetramer of two alpha and two beta subunits By similarity. SAAS SAAS002547 HAMAP-Rule MF_00283

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00283.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. HAMAP-Rule MF_00283

Contains 1 B5 domain. SAAS SAAS002547 HAMAP-Rule MF_00283

Contains 1 FDX-ACB domain. SAAS SAAS002547 HAMAP-Rule MF_00283

Contains 1 tRNA-binding domain. SAAS SAAS002547 HAMAP-Rule MF_00283

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain44 – 160117tRNA-binding By similarity HAMAP-Rule MF_00283
Domain420 – 49576B5 By similarity HAMAP-Rule MF_00283
Domain742 – 83594FDX-ACB By similarity HAMAP-Rule MF_00283

Sites

Metal binding4731Magnesium By similarity HAMAP-Rule MF_00283
Metal binding4791Magnesium; via carbonyl oxygen By similarity HAMAP-Rule MF_00283
Metal binding4821Magnesium By similarity HAMAP-Rule MF_00283
Metal binding4831Magnesium By similarity HAMAP-Rule MF_00283

Sequences

Sequence LengthMass (Da)Tools
H2I9E9 [UniParc].

Last modified March 21, 2012. Version 1.
Checksum: BAD46324EF5308C5

FASTA83689,753
        10         20         30         40         50         60 
MLISQNWVTE LLGRSNPDWK VSPAELDSGY VRVGFETEGY SAIPETTGPL VIGRVETIEE 

        70         80         90        100        110        120 
LEGFKKPIRH CFVNVGDANG TGELQSIVCG ARNFQEGSYV VVSLPGAVLP GNFAISARET 

       130        140        150        160        170        180 
YGRMSAGMIC SAAELGLSDK QNPGIITLPN EIAEPGTDAR SIVGLDDTVF DVNITPDRGY 

       190        200        210        220        230        240 
ALSARGLTRE LASAFNLKFV DPAVDLNVAG VDTSDVPEPS GELIKVDLRP ETQARRFGVR 

       250        260        270        280        290        300 
KISGIDPKAP TPFWMQRELM LSGQRCVNAA TDVTNFVMLL LGQPMHAFDA NLIKGGLVVR 

       310        320        330        340        350        360 
NALEGESFET LDHVKRTLSA EDVVISDDAG IQSLAGVMGG TTSEISDETI DVFFEAANWH 

       370        380        390        400        410        420 
PITTARTSRR HKLSTEASRR FERGVDPEII EVALDVACAL LVSIAGGSVE SARTLIGSAP 

       430        440        450        460        470        480 
SMPQIRMKTS RPAELAGVAY SDATVIARLK EVGCAVEVDG DDLLVTPPTW RPDMTMSADL 

       490        500        510        520        530        540 
VEEVLRLEGL EDIPAIVPLA PVGSGLSPAQ LRRRAIGHAL AYSGYAEILP TPFIRNDTFD 

       550        560        570        580        590        600 
VWGLAADDER RSVVTVQNPL DAEYGVLATT LLPSMLEAVT RNVARGQTSV NLFGLQQVSF 

       610        620        630        640        650        660 
KRGSGISPML DVRQRPSDNE VAELLKSLPV QPLHVATVGA GFMELDGPWG HGRTYSFADA 

       670        680        690        700        710        720 
IESARVVARA AGVELNVENV EMLPWHPGRC AALKAGDHIV GYAGELHPQI VEALNLPART 

       730        740        750        760        770        780 
CAMELDVSAL PLKESFPAPV LSAFPVLHQD LALVVDESVP AESVRKVIED AAGELLEKVE 

       790        800        810        820        830 
LFDVYRSEAL GAEKKSLAFS LEFRAQDRTL TDDECSEGRL AAAGRAAELF GATMRA

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References

[1]"Pangenomic study of Corynebacterium diphtheriae that provides insights into the genomic diversity of pathogenic isolates from cases of classical diphtheria, endocarditis, and pneumonia."
Trost E., Blom J., de Castro Soares S., Huang I.H., Al-Dilaimi A., Schroder J., Jaenicke S., Dorella F.A., Rocha F.S., Miyoshi A., Azevedo V., Schneider M.P., Silva A., Camello T.C., Sabbadini P.S., Santos C.S., Santos L.S., Hirata R. Jr. expand/collapse author list , Mattos-Guaraldi A.L., Efstratiou A., Schmitt M.P., Ton-That H., Tauch A.
J. Bacteriol. 194:3199-3215(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VA01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003217 Genomic DNA. Translation: AEX83307.1.
RefSeqYP_005144947.1. NC_016790.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEX83307; AEX83307; CDVA01_1038.
GeneID11718258.
KEGGcdv:CDVA01_1038.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01890.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPMF_00283. Phe_tRNA_synth_beta1.
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu_bac.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMSSF56037. B3_4. 1 hit.
SSF54991. Fdx_AntiC_bd. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMsTIGR00472. pheT_bact. 1 hit.
PROSITEPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH2I9E9_CORDV
AccessionPrimary (citable) accession number: H2I9E9
Entry history
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: April 3, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info