Skip Header

Contribute Send feedback
Read comments (?) or add your own

H2GCI5 (H2GCI5_CORD2) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase RuleBase RU003777
EC=2.4.2.29 RuleBase RU003777
Gene names
Name:tgt EMBL AEX43272.1
Ordered Locus Names:CD241_0205 EMBL AEX43272.1
OrganismCorynebacterium diphtheriae (strain 241) [Complete proteome] [HAMAP] EMBL AEX43272.1
Taxonomic identifier698966 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium ›

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. RuleBase RU003777 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. SAAS SAAS004803 RuleBase RU003777

Pathway

tRNA modification; tRNA-queuosine biosynthesis. SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. RuleBase RU003777

Sequences

Sequence LengthMass (Da)Tools
H2GCI5 [UniParc].

Last modified March 21, 2012. Version 1.
Checksum: B95D823C92B63380

FASTA43047,688
        10         20         30         40         50         60 
MTDTTFELRT ELDDAPGRHG RTGVIHTPHG DINTPAFIPV ATKATVKTLT PQQIRDTGAQ 

        70         80         90        100        110        120 
AILSNAYHLY LQPGPDIVDE AGGVSKFENW HGPTYTDSGG FQVMSLGVGF RKVLAMDTAG 

       130        140        150        160        170        180 
LVEGDIRAPK KDRYAQVDED GVDFRSVIDG SRHRFTPEIS MQIQHQLGAD IIFAFDELTT 

       190        200        210        220        230        240 
LVDTRQYQES SVERTHRWAR RCLIEHDRLT REREGKPLQS LWGVVQGAQY EDLRRQAIRG 

       250        260        270        280        290        300 
LLDLDKEAED NGRRGFGGFG IGGALEKENL GTIVGWVCDE IPQHKPRHLL GISEPDDLFT 

       310        320        330        340        350        360 
AIEAGADTFD CVAPTRLGRR GGVYTLDGRV NLTAARFKRD FRQVDEEFGG PIAEYSRAYI 

       370        380        390        400        410        420 
HHLFKAKEFL GGTLCTMHNV AFMIQLVDNI RAAINNGDFE AYRDEFLGRY YASKGGPAAA 

       430 
GRSGGMIYGV

« Hide

References

[1]"Pangenomic Study of Corynebacterium diphtheriae That Provides Insights into the Genomic Diversity of Pathogenic Isolates from Cases of Classical Diphtheria, Endocarditis, and Pneumonia."
Trost E., Blom J., de Castro Soares S., Huang I.H., Al-Dilaimi A., Schroder J., Jaenicke S., Dorella F.A., Rocha F.S., Miyoshi A., Azevedo V., Schneider M.P., Silva A., Camello T.C., Sabbadini P.S., Santos C.S., Santos L.S., Hirata R.Jr. expand/collapse author list , Mattos-Guaraldi A.L., Efstratiou A., Schmitt M.P., Ton-That H., Tauch A.
J. Bacteriol. 194:3199-3215(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 241 EMBL AEX43272.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003207 Genomic DNA. Translation: AEX43272.1.
RefSeqYP_005124474.1. NC_016782.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEX43272; AEX43272; CD241_0205.
GeneID11673176.
KEGGcdp:CD241_0205.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00773.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameH2GCI5_CORD2
AccessionPrimary (citable) accession number: H2GCI5
Entry history
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: May 29, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info