H2G3M1 (H2G3M1_CORD3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
5-methyltetrahydrofolate-homocysteinemethyltransferase EMBL AEX41945.1
EC=2.1.1.13 EMBL AEX41945.1

Gene names

Name:	metH EMBL AEX41945.1
Ordered Locus Names:	CD31A_1270 EMBL AEX41945.1

Organism

Corynebacterium diphtheriae (strain 31A) [Complete proteome] [HAMAP] EMBL AEX41945.1

Taxonomic identifier

698962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium diphtheriae

Protein attributes

Sequence length	1200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor

Binds 1 zinc ion per subunit By similarity. PIRSR PIRSR000381-1 Cobalamin By similarity. PIRSR PIRSR000381-2

Ontologies

Keywords
Ligand	Cobalamin PIRSR PIRSR000381-2 Cobalt Metal-binding PIRSR PIRSR000381-1 S-adenosyl-L-methionine PIRSR PIRSR000381-2 Zinc PIRSR PIRSR000381-1
Molecular function	Methyltransferase EMBL AEX41945.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro methionine synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	827 – 828	2	Cobalamin-binding By similarity PIRSR PIRSR000381-2
	Region	1197 – 1198	2	S-adenosyl-L-methionine binding By similarity PIRSR PIRSR000381-2
Sites
	Metal binding	233	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	299	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	300	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	751	1	Cobalt (cobalamin axial ligand) By similarity PIRSR PIRSR000381-1
	Binding site	948	1	S-adenosyl-L-methionine By similarity PIRSR PIRSR000381-2
	Binding site	1143	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity PIRSR PIRSR000381-2
	Binding site	1147	1	Cobalamin; via carbonyl oxygen By similarity PIRSR PIRSR000381-2

Sequences

	Sequence		Length	Mass (Da)
	H2G3M1 [UniParc]. Last modified March 21, 2012. Version 1. Checksum: A4B46E1F8433A8B4	FASTA	1,200	131,334
	10 20 30 40 50 60 MNTATPQFKT AFLDAMKNRV LIGDGAMGTQ LQGFDLDVDS DFLGLEGCNE ILNHTRPDVV 70 80 90 100 110 120 SAIHRAYFEA GADLVETNTF GCNLPNLADY DIADRCKELA FKGVKIAREV ADELGLSADG 130 140 150 160 170 180 TPRFVLGSMG PGTKLPSLGH APYEALKNHY TEASLGMIEG GADAILVETA QDLLQVKAAV 190 200 210 220 230 240 HGCQTAFETS GIQLPIVCHV TVETTGTMLL GSEIGAALTA IEPLNVDMIG LNCATGPDEM 250 260 270 280 290 300 SEHLRYLSRN AHIPVSVMPN AGLPILGKNG AEYPLTEQEL ASALRGFVEE YGLSMVGGCC 310 320 330 340 350 360 GTTPTHIREV RKAVLGDSEH QPALQAERNP DVGDAVSSLY TSVNLTQDTG ITMIGERTNA 370 380 390 400 410 420 NGSKAFREAM LVGDLEKCVD IAKQQTRDGA HMLDLCVDYV GRDGTADMAQ LASLLATSST 430 440 450 460 470 480 LPIMIDSTEP DVIQVGLEHL GGRCAVNSVN FEDGDGPNSR YQRIMRLVKR HGAAVVALTI 490 500 510 520 530 540 DEEGQARTAE KKIEIAERLI ADITSTWGLD ESDIIVDTLT FPISTGQEET RRDGIETIEA 550 560 570 580 590 600 IRELKKRHPK IHTTLGLSNI SFGLNPAARQ VLNSVFLNEC IEAGLDSAIA HSSKIVPMNR 610 620 630 640 650 660 IDEEQRRVAL DMVYDRRSEG YDPLQTFMRL FEGVSAASAS DARAEALAAM PLFERIAQRI 670 680 690 700 710 720 IDGEKAGFED DLDAGMKEKE PLQIINEDLL EGMKTVGDLF GSGQMQLPFV LQSAETMKHA 730 740 750 760 770 780 VAYLEQFIEA DEDAGDGNGT IVIATVKGDV HDIGKNLVDI ILSNNGFNVV NIGIKQPIAN 790 800 810 820 830 840 ILEAAEKHKA DAIGMSGLLV KSTVIMKENL QEMNQAQKSD YPVILGGAAL TRAYVEDDLT 850 860 870 880 890 900 EVYEGNVHYA KDAFESLRLM QEFMAEARGE RLDPQSPEAL AAAKKKAERK ARRERSKQIV 910 920 930 940 950 960 AQRKAKEIPI EVPERSEVAA DVPIATPPFW GTRIVKGINL SEYLPLLDER ALFVGRWGLK 970 980 990 1000 1010 1020 ATRGGEGPSY EELVETEGRP RLRYWIDRLK AEKILDHAAV VYGYFPAVSE GDTVILLESP 1030 1040 1050 1060 1070 1080 DPTSAEVARF DFPRQQRGKF LCVADFIRSR EHAIKTKTVD VFPLQLVTMG QPIADFANVL 1090 1100 1110 1120 1130 1140 FADNNYRDYL EVHGVGVQLT EALAEYWHAR IRHELSLSDG SHAGDEDSAD LQEFFNLKYR 1150 1160 1170 1180 1190 1200 GARYSFGYGS CPNLEDRETL VDLLDSSRIG VDISEEFQLH PEQSTDAFVL YHPEAKYFNV

References

[1]

"Pangenomic Study of Corynebacterium diphtheriae That Provides Insights into the Genomic Diversity of Pathogenic Isolates from Cases of Classical Diphtheria, Endocarditis, and Pneumonia." Trost E., Blom J., de Castro Soares S., Huang I.H., Al-Dilaimi A., Schroder J., Jaenicke S., Dorella F.A., Rocha F.S., Miyoshi A., Azevedo V., Schneider M.P., Silva A., Camello T.C., Sabbadini P.S., Santos C.S., Santos L.S., Hirata R.Jr. , Mattos-Guaraldi A.L., Efstratiou A., Schmitt M.P., Ton-That H., Tauch A. J. Bacteriol. 194:3199-3215(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 31A EMBL AEX41945.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003206 Genomic DNA. Translation: AEX41945.1.
RefSeq	YP_005157972.1. NC_016799.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEX41945; AEX41945; CD31A_1270.
GeneID	11732817.
KEGG	cdz:CD31A_1270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K00548.
Enzyme and pathway databases
BioCyc	CDIP698962:GH9X-1300-MONOMER.
Family and domain databases
Gene3D	1.10.1240.10. 1 hit. 3.10.196.10. 1 hit. 3.20.20.20. 1 hit. 3.20.20.330. 1 hit. 3.40.50.280. 1 hit.
InterPro	IPR003759. Cbl-bd_cap. IPR006158. Cobalamin-bd. IPR011005. Dihydropteroate_synth-like. IPR011822. MetH. IPR000489. Pterin-binding. IPR003726. S_MeTrfase. IPR004223. VitB12-dep_Met_synth_activ_dom. [Graphical view]
Pfam	PF02310. B12-binding. 1 hit. PF02607. B12-binding_2. 1 hit. PF02965. Met_synt_B12. 1 hit. PF00809. Pterin_bind. 1 hit. PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF000381. MetH. 1 hit.
SMART	SM01018. B12-binding_2. 1 hit. [Graphical view]
SUPFAM	SSF52242. Cbl-bd. 1 hit. SSF51717. DHP_synth_like. 1 hit. SSF56507. Met_synth_B12. 1 hit. SSF47644. Met_synth_Cbl-bd. 1 hit. SSF82282. S_methyl_trans. 1 hit.
TIGRFAMs	TIGR02082. metH. 1 hit.
PROSITE	PS50974. ADOMET_ACTIVATION. 1 hit. PS51332. B12_BINDING. 1 hit. PS51337. B12_BINDING_NTER. 1 hit. PS50970. HCY. 1 hit. PS50972. PTERIN_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H2G3M1_CORD3

Accession

Primary (citable) accession number: H2G3M1

Entry history

Integrated into UniProtKB/TrEMBL:	March 21, 2012
Last sequence update:	March 21, 2012
Last modified:	May 29, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)