H2FY32 (H2FY32_OCESG) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049 EMBL AEY00989.1
Ordered Locus Names:	GU3_06165 EMBL AEY00989.1

Organism

Oceanimonas sp. (strain GK1) [Complete proteome] [HAMAP] EMBL AEY00989.1

Taxonomic identifier

511062 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Oceanimonas

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049 RuleBase RU003489
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 RuleBase RU003489 EMBL AEY00989.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	42 – 52	11	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	619 – 623	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	622	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	H2FY32 [UniParc]. Last modified March 21, 2012. Version 1. Checksum: 40C7EA3E3441C5C5	FASTA	859	97,054
	10 20 30 40 50 60 MQEQYNPQNI EPQVQRHWDT QQTFKATEQP GKDKFYCLSM FPYPSGRLHM GHVRNYTIGD 70 80 90 100 110 120 VISRYQRLQG KNVLQPIGWD AFGLPAENAA INNKTAPAPW TYENIDYMKN QLKRLGFGYD 130 140 150 160 170 180 WDRELATCTP EYYRWEQWFF TKLYEKGLVY KKTSSVNWCP NDETVLANEQ VNDGCCWRCD 190 200 210 220 230 240 TPVEQKEIPQ WFIKITDYAD ELLRDIDELE GWPEQVKTMQ RNWIGRSEGV TLRFDVAGAD 250 260 270 280 290 300 DGFEVYTTRP DTLFGVTYVG IAAGHPLAAK AAENNPALAA FIDDCKHNKV AEADMATMEK 310 320 330 340 350 360 KGMATGLMAV HPLTGKQVPI WVANFVLMDY GSGAVMAVPA HDQRDYEFAT KYGLDIVPVI 370 380 390 400 410 420 KPADGSELDV SQEAFTEKGL LFHSNEFDGM NFEQAFDAIA RTLEEKGLGR RTVNFRLRDW 430 440 450 460 470 480 GVSRQRYWGA PIPMLNLEDG SVVGAPEESL PVVLPEDVVM DGIQSPIKAD PEWAKTTYHG 490 500 510 520 530 540 QPALRETDTF DTFMESSWYY ARYCSPDYDQ GMLDVEKANY WLPVDQYIGG IEHACMHLLY 550 560 570 580 590 600 ARFFHKLLRD TGLVESDEPF KRLLCQGMVL ADAFYHIDDK GGRTWVSPLD VTVERDEKGR 610 620 630 640 650 660 ISKATDKDGR ELVHTGMTKM SKSKNNGIDP QVMVDKYGAD TVRLFMMFAS PADMTLEWSD 670 680 690 700 710 720 SGVEGAQRFL KRLWKLVFEH QSHGAAPALN LSGLSSEQKT LRRELHKTIA KVSDDVGRRQ 730 740 750 760 770 780 TFNTAIAAIM ELMNHLAKVD MSDEQNRALL QEALEAVVVM LHPIVPHACF ELWRALGHTD 790 800 810 820 830 840 IDHAAWPVVD AEALVEDEKL VVVQVNGKVR GKVTVPADAS QDAVESAGKA DDNVARHLDG 850 KTIRKVIYVP GKLLNIVAN

References

[1]

"Complete genome sequence of Oceanimonas sp. GK1, a halotolerant bacterium from Gavkhouni Wetland in Iran." Parsa Yeganeh L., Azarbaijani R., Sarikhan S., Mousavi H., Ramezani M., Amoozegar M.A., Shahzadeh Fazeli A., Salekdeh G.H. J. Bacteriol. 194:2123-2124(2012) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GK1 EMBL AEY00989.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003171 Genomic DNA. Translation: AEY00989.1.
RefSeq	YP_005091739.1. NC_016745.1.
3D structure databases
ProteinModelPortal	H2FY32.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEY00989; AEY00989; GU3_06165.
GeneID	11599033.
KEGG	oce:GU3_06165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01869.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 3 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H2FY32_OCESG

Accession

Primary (citable) accession number: H2FY32

Entry history

Integrated into UniProtKB/TrEMBL:	March 21, 2012
Last sequence update:	March 21, 2012
Last modified:	April 3, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)