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H2A0N4

- PIF_PINMG

UniProt

H2A0N4 - PIF_PINMG

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Protein

Protein PIF

Gene
N/A
Organism
Pinctada margaritifera (Black-lipped pearl oyster)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the organic matrix for normal growth of the nacreous layer. The complex contributes to the initiation of aragonite crystallization as well as subsequenct stacking of aragonite tablets in the nacreous layer (By similarity).By similarity
Pif80 binds to both aragonite and calcite crystals, with a higher specificity for aragonite crystals.By similarity
Pif97 contains a chitin-binding domain that allows for attachment of the entire complex to the chitin-containing organic framework.By similarity

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. chitin metabolic process Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Chitin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PIF
Cleaved into the following 2 chains:
Alternative name(s):
Aragonite-binding proteinBy similarity
OrganismiPinctada margaritifera (Black-lipped pearl oyster)
Taxonomic identifieri102329 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaPterioidaPterioideaPteriidaePinctada

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 558536Protein Pif97PRO_0000418022Add
BLAST
Chaini559 – 1014456Protein Pif80PRO_0000418023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi298 ↔ 311PROSITE-ProRule annotation
Disulfide bondi354 ↔ 366PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Nacreous layer of shell (at protein level). Expressed primarily in the mantle with highest level in the mantle pallium and lower level in the mantle edge.1 Publication

Interactioni

Subunit structurei

Heterooligomer; disulfide-linked. Pif97, Pif80, N16 and other proteins form a complex (By similarity).By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 202174VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini257 – 374118Chitin-binding type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni555 – 5584Kex2-like proteinase cleavage siteBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi369 – 990622Asp-richSequence AnalysisAdd
BLAST
Compositional biasi555 – 63682Arg-richSequence AnalysisAdd
BLAST
Compositional biasi706 – 834129Lys-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR002557. Chitin-bd_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 2 hits.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

H2A0N4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVPYLQIVF LLTAVFGIGV KSDDCKTADL VVNVDGSDDV SDREFDKLKR
60 70 80 90 100
AMLMLVRGLS IDDSQIRLGM VTYGSEIGDS IPLQGDRLDL ARTIRYMKKP
110 120 130 140 150
GGPCKPFKGI GETRKMFSSR GRFNVPHVTL NLGGDIVDSE VRDLMDETDK
160 170 180 190 200
ARDEDIKVMA IGLGTKVERD EIEGIAWDKE QAYFMDDADD LVRRVKEIPD
210 220 230 240 250
YLCKIIKAKK PRKSASKKSK TKPAKKPDSD IVGKSPGFHS LQRTDDKPKM
260 270 280 290 300
SKKVEVKELC DDAEWVEDVG YGSVPTRCED FVMCQNVSGS LRKTLKTCPY
310 320 330 340 350
GQFWSRARTS CVLTEDEDCS DDLCKTMLLP SRDYDVSCRA YWKCENGKSV
360 370 380 390 400
ARCCPSGMAY EPGKGCVLDS DCDEECPPKG DSDNGDDDDD DNDDDDNEYD
410 420 430 440 450
DDDDEMEYNP NCPLRPIKGS PEKFKQHTGD DNWEEFDCAP GTLFSSRDCA
460 470 480 490 500
CSILGRPEKD DNGKNEDDTS KVCEPELYLP FCDDLHDYSG KETHVENEGD
510 520 530 540 550
AVIIENGKAY FNGRAGLKIP RFSGVPYGKS VFIKMKYKED EDDDKKRNDD
560 570 580 590 600
DKKLRIKRDE RGRKGYRKGG RKDDRNGKRR DDRIGKRRDD RIFDDRKGRR
610 620 630 640 650
TDDRKGDRRD RIDDRNGRRT DDRKDNRRDD RKDNRRDDIK DNKRDDKKDN
660 670 680 690 700
ADEPMTLISN GECDNFELHD CFEKPSLAIT TGKKFAGFSV SSTERDEVDL
710 720 730 740 750
EVDNDKKGYL WNKDKKDKDD KNRRDKDKNG DRTDDKKSLD DLVKEIERRK
760 770 780 790 800
SDDKKSFDDL VKEIERRKSD DKKSFDDLVK EIERRKSDDK ISLDDLVKEI
810 820 830 840 850
KRRKSDDKGN GRRKDDNKND EDDDKKGWKT VSLKINNGHI RGRRDDREDK
860 870 880 890 900
DIMDGDLKTT FSGFQIGQGA SNKNFKGYMD EVYIYFCDPG KEADFDEEDD
910 920 930 940 950
NGDDDDDDDD DDDKDNDAGD DNKDDNNNNG RKDDNNNDGR KDDNNKKDDK
960 970 980 990 1000
DRSDKNGGKD DKDKDTKDKF SDKDNGKDNE DADRDINDND KLYRRAMKKC
1010
DFVNKNVEKW LDKR
Length:1,014
Mass (Da):116,591
Last modified:March 21, 2012 - v1
Checksum:i7C4F1E7FA9555BA8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE610401 mRNA. Translation: CCE46175.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE610401 mRNA. Translation: CCE46175.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.140.10. 1 hit.
3.40.50.410. 1 hit.
InterProi IPR002557. Chitin-bd_dom.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF01607. CBM_14. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view ]
SMARTi SM00494. ChtBD2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEi PS50940. CHIT_BIND_II. 2 hits.
PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization."
    Joubert C., Piquemal D., Marie B., Manchon L., Pierrat F., Zanella-Cleon I., Cochennec-Laureau N., Gueguen Y., Montagnani C.
    BMC Genomics 11:613-613(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION.
    Tissue: Mantle.
  2. "Different secretory repertoires control the biomineralization processes of prism and nacre deposition of the pearl oyster shell."
    Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C., Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.
    Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 51-87; 99-108; 123-152; 158-166; 170-193; 226-243; 297-306; 309-339; 353-364; 426-459; 472-491; 522-529; 791-798; 848-873 AND 989-995, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Shell.

Entry informationi

Entry nameiPIF_PINMG
AccessioniPrimary (citable) accession number: H2A0N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: March 21, 2012
Last modified: October 29, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Pif80 and Pif97 are rich in Asp. This amino-acid appears to be common to biomineral-forming organisms (By similarity).By similarity

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3