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H2A0N4

- PIF_PINMG

UniProt

H2A0N4 - PIF_PINMG

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Protein
Protein PIF
Gene
N/A
Organism
Pinctada margaritifera (Black-lipped pearl oyster)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Essential component of the organic matrix for normal growth of the nacreous layer. The complex contributes to the initiation of aragonite crystallization as well as subsequenct stacking of aragonite tablets in the nacreous layer By similarity.By similarity
Pif80 binds to both aragonite and calcite crystals, with a higher specificity for aragonite crystals By similarity.By similarity
Pif97 contains a chitin-binding domain that allows for attachment of the entire complex to the chitin-containing organic framework By similarity.By similarity

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. chitin metabolic process Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Chitin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PIF
Cleaved into the following 2 chains:
Alternative name(s):
Aragonite-binding protein
OrganismiPinctada margaritifera (Black-lipped pearl oyster)
Taxonomic identifieri102329 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaPterioidaPterioideaPteriidaePinctada

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 558536Protein Pif97
PRO_0000418022Add
BLAST
Chaini559 – 1014456Protein Pif80
PRO_0000418023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi298 ↔ 311 By similarity
Disulfide bondi354 ↔ 366 By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Nacreous layer of shell (at protein level). Expressed primarily in the mantle with highest level in the mantle pallium and lower level in the mantle edge.1 Publication

Interactioni

Subunit structurei

Heterooligomer; disulfide-linked. Pif97, Pif80, N16 and other proteins form a complex By similarity.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 202174VWFA
Add
BLAST
Domaini257 – 374118Chitin-binding type-2By similarity
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni555 – 5584Kex2-like proteinase cleavage site By similarityBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi369 – 990622Asp-rich
Add
BLAST
Compositional biasi555 – 63682Arg-rich
Add
BLAST
Compositional biasi706 – 834129Lys-rich
Add
BLAST

Sequence similaritiesi

Contains 1 VWFA domain.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR002557. Chitin-bd_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 2 hits.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

H2A0N4-1 [UniParc]FASTAAdd to Basket

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MQVPYLQIVF LLTAVFGIGV KSDDCKTADL VVNVDGSDDV SDREFDKLKR     50
AMLMLVRGLS IDDSQIRLGM VTYGSEIGDS IPLQGDRLDL ARTIRYMKKP 100
GGPCKPFKGI GETRKMFSSR GRFNVPHVTL NLGGDIVDSE VRDLMDETDK 150
ARDEDIKVMA IGLGTKVERD EIEGIAWDKE QAYFMDDADD LVRRVKEIPD 200
YLCKIIKAKK PRKSASKKSK TKPAKKPDSD IVGKSPGFHS LQRTDDKPKM 250
SKKVEVKELC DDAEWVEDVG YGSVPTRCED FVMCQNVSGS LRKTLKTCPY 300
GQFWSRARTS CVLTEDEDCS DDLCKTMLLP SRDYDVSCRA YWKCENGKSV 350
ARCCPSGMAY EPGKGCVLDS DCDEECPPKG DSDNGDDDDD DNDDDDNEYD 400
DDDDEMEYNP NCPLRPIKGS PEKFKQHTGD DNWEEFDCAP GTLFSSRDCA 450
CSILGRPEKD DNGKNEDDTS KVCEPELYLP FCDDLHDYSG KETHVENEGD 500
AVIIENGKAY FNGRAGLKIP RFSGVPYGKS VFIKMKYKED EDDDKKRNDD 550
DKKLRIKRDE RGRKGYRKGG RKDDRNGKRR DDRIGKRRDD RIFDDRKGRR 600
TDDRKGDRRD RIDDRNGRRT DDRKDNRRDD RKDNRRDDIK DNKRDDKKDN 650
ADEPMTLISN GECDNFELHD CFEKPSLAIT TGKKFAGFSV SSTERDEVDL 700
EVDNDKKGYL WNKDKKDKDD KNRRDKDKNG DRTDDKKSLD DLVKEIERRK 750
SDDKKSFDDL VKEIERRKSD DKKSFDDLVK EIERRKSDDK ISLDDLVKEI 800
KRRKSDDKGN GRRKDDNKND EDDDKKGWKT VSLKINNGHI RGRRDDREDK 850
DIMDGDLKTT FSGFQIGQGA SNKNFKGYMD EVYIYFCDPG KEADFDEEDD 900
NGDDDDDDDD DDDKDNDAGD DNKDDNNNNG RKDDNNNDGR KDDNNKKDDK 950
DRSDKNGGKD DKDKDTKDKF SDKDNGKDNE DADRDINDND KLYRRAMKKC 1000
DFVNKNVEKW LDKR 1014
Length:1,014
Mass (Da):116,591
Last modified:March 21, 2012 - v1
Checksum:i7C4F1E7FA9555BA8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE610401 mRNA. Translation: CCE46175.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE610401 mRNA. Translation: CCE46175.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.140.10. 1 hit.
3.40.50.410. 1 hit.
InterProi IPR002557. Chitin-bd_dom.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF01607. CBM_14. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view ]
SMARTi SM00494. ChtBD2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEi PS50940. CHIT_BIND_II. 2 hits.
PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization."
    Joubert C., Piquemal D., Marie B., Manchon L., Pierrat F., Zanella-Cleon I., Cochennec-Laureau N., Gueguen Y., Montagnani C.
    BMC Genomics 11:613-613(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION.
    Tissue: Mantle.
  2. "Different secretory repertoires control the biomineralization processes of prism and nacre deposition of the pearl oyster shell."
    Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C., Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.
    Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 51-87; 99-108; 123-152; 158-166; 170-193; 226-243; 297-306; 309-339; 353-364; 426-459; 472-491; 522-529; 791-798; 848-873 AND 989-995, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Shell.

Entry informationi

Entry nameiPIF_PINMG
AccessioniPrimary (citable) accession number: H2A0N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: March 21, 2012
Last modified: April 16, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Pif80 and Pif97 are rich in Asp. This amino-acid appears to be common to biomineral-forming organisms By similarity.By similarity

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi