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H1AFK5

- H1AFK5_SHEFR

UniProt

H1AFK5 - H1AFK5_SHEFR

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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Shewanella frigidimarina
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei50 – 501Proton acceptorUniRule annotation
Active sitei61 – 611Proton acceptorUniRule annotation
Binding sitei214 – 2141Sulfate 2; via amide nitrogenImported
Binding sitei236 – 2361Sulfate 2Imported

GO - Molecular functioni

  1. tryptophan synthase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotationImported
OrganismiShewanella frigidimarinaImported
Taxonomic identifieri56812 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotationSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VNDX-ray2.60A/B/C/D/E/F/G/H1-267[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 953Sulfate 1 bindingImported

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

H1AFK5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNRYQAKFA ALKAQDKGAF VPFVTIGDPS PELSLKIIQT LVDNGADALE
60 70 80 90 100
LGFPFSDPLA DGPVIQGANL RSLAAGTTSS DCFDIITKVR AQHPDMPIGL
110 120 130 140 150
LLYANLVFAN GIDEFYTKAQ AAGVDSVLIA DVPVEESAPF SKAAKAHGIA
160 170 180 190 200
PIFIAPPNAD ADTLKMVSEQ GEGYTYLLSR AGVTGTESKA GEPIENILTQ
210 220 230 240 250
LAEFNAPPPL LGFGIAEPEQ VRAAIKAGAA GAISGSAVVK IIEAHQHDEA
260
TLLAKLAEFT TAMKAAT
Length:267
Mass (Da):27,806
Last modified:March 21, 2012 - v1
Checksum:iFC55FDE6C55A3E40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB690266 Genomic DNA. Translation: BAL45195.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB690266 Genomic DNA. Translation: BAL45195.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VND X-ray 2.60 A/B/C/D/E/F/G/H 1-267 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00044 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00131. Trp_synth_alpha.
InterProi IPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view ]
Pfami PF00290. Trp_syntA. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR00262. trpA. 1 hit.
PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Ishdia M., Mita M.
    Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: K14-2Imported.
  2. "Strategy for cold adaptation of the tryptophan synthase alpha subunit from the psychrophile Shewanella frigidimarina K14-2: Crystal structure and physicochemical properties."
    Mitsuya D., Tanaka S., Matsumura H., Urano N., Takano K., Ogasahara K., Takehira M., Yutani K., Ishida M.
    Submitted (JAN-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
  3. "The crystal strucutre of the tryptophan synthase alpha subunit from the psychrophile Shewanella frigidimarina."
    Mitsuya D., Tanaka S., Matsumura H., Urano N., Takano K., Ogasahara K., Yutani K., Ishida M.
    Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: K14-2Imported.

Entry informationi

Entry nameiH1AFK5_SHEFR
AccessioniPrimary (citable) accession number: H1AFK5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 21, 2012
Last sequence update: March 21, 2012
Last modified: November 26, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3