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H1AFK5

- H1AFK5_SHEFR

UniProt

H1AFK5 - H1AFK5_SHEFR

Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Shewanella frigidimarina
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (21 Mar 2012)
      Previous versions | rss
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    Functioni

    The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotationSAAS annotation

    Catalytic activityi

    L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei50 – 501Proton acceptorUniRule annotation
    Active sitei61 – 611Proton acceptorUniRule annotation
    Binding sitei214 – 2141Sulfate 2; via amide nitrogenImported
    Binding sitei236 – 2361Sulfate 2Imported

    GO - Molecular functioni

    1. tryptophan synthase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LyaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesisUniRule annotationSAAS annotation

    Enzyme and pathway databases

    UniPathwayiUPA00035; UER00044.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
    Gene namesi
    Name:trpAUniRule annotationImported
    OrganismiShewanella frigidimarinaImported
    Taxonomic identifieri56812 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VNDX-ray2.60A/B/C/D/E/F/G/H1-267[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 953Sulfate 1 bindingImported

    Sequence similaritiesi

    Belongs to the TrpA family.UniRule annotation

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00131. Trp_synth_alpha.
    InterProiIPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view]
    PfamiPF00290. Trp_syntA. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    TIGRFAMsiTIGR00262. trpA. 1 hit.
    PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    H1AFK5-1 [UniParc]FASTAAdd to Basket

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    MSNRYQAKFA ALKAQDKGAF VPFVTIGDPS PELSLKIIQT LVDNGADALE    50
    LGFPFSDPLA DGPVIQGANL RSLAAGTTSS DCFDIITKVR AQHPDMPIGL 100
    LLYANLVFAN GIDEFYTKAQ AAGVDSVLIA DVPVEESAPF SKAAKAHGIA 150
    PIFIAPPNAD ADTLKMVSEQ GEGYTYLLSR AGVTGTESKA GEPIENILTQ 200
    LAEFNAPPPL LGFGIAEPEQ VRAAIKAGAA GAISGSAVVK IIEAHQHDEA 250
    TLLAKLAEFT TAMKAAT 267
    Length:267
    Mass (Da):27,806
    Last modified:March 21, 2012 - v1
    Checksum:iFC55FDE6C55A3E40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB690266 Genomic DNA. Translation: BAL45195.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB690266 Genomic DNA. Translation: BAL45195.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VND X-ray 2.60 A/B/C/D/E/F/G/H 1-267 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00044 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00131. Trp_synth_alpha.
    InterProi IPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view ]
    Pfami PF00290. Trp_syntA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    TIGRFAMsi TIGR00262. trpA. 1 hit.
    PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Ishdia M., Mita M.
      Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: K14-2Imported.
    2. "Strategy for cold adaptation of the tryptophan synthase alpha subunit from the psychrophile Shewanella frigidimarina K14-2: Crystal structure and physicochemical properties."
      Mitsuya D., Tanaka S., Matsumura H., Urano N., Takano K., Ogasahara K., Takehira M., Yutani K., Ishida M.
      Submitted (JAN-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
    3. "The crystal strucutre of the tryptophan synthase alpha subunit from the psychrophile Shewanella frigidimarina."
      Mitsuya D., Tanaka S., Matsumura H., Urano N., Takano K., Ogasahara K., Yutani K., Ishida M.
      Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: K14-2Imported.

    Entry informationi

    Entry nameiH1AFK5_SHEFR
    AccessioniPrimary (citable) accession number: H1AFK5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 21, 2012
    Last sequence update: March 21, 2012
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3