H0PBQ8 (H0PBQ8_9SYNC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
5-methyltetrahydrofolate--homocysteine methyltransferase EMBL BAL33390.1

Gene names

Name:	metH EMBL BAL33390.1
ORF Names:	SYNPCCN_2473 EMBL BAL33390.1

Organism

Synechocystis sp. PCC 6803 substr. PCC-N EMBL BAL33390.1

Taxonomic identifier

1080229 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	1195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor

Binds 1 zinc ion per subunit By similarity. PIRSR PIRSR000381-1 Cobalamin By similarity. PIRSR PIRSR000381-2

Ontologies

Keywords
Ligand	Cobalamin PIRSR PIRSR000381-2 Cobalt Metal-binding PIRSR PIRSR000381-1 S-adenosyl-L-methionine PIRSR PIRSR000381-2 Zinc PIRSR PIRSR000381-1
Molecular function	Methyltransferase EMBL BAL33390.1 Transferase
Gene Ontology (GO)
Biological_process	pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro methionine synthase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	812 – 813	2	Cobalamin-binding By similarity PIRSR PIRSR000381-2
	Region	1192 – 1193	2	S-adenosyl-L-methionine binding By similarity PIRSR PIRSR000381-2
Sites
	Metal binding	228	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	294	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	295	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	737	1	Cobalt (cobalamin axial ligand) By similarity PIRSR PIRSR000381-1
	Binding site	945	1	S-adenosyl-L-methionine By similarity PIRSR PIRSR000381-2
	Binding site	1138	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity PIRSR PIRSR000381-2
	Binding site	1142	1	Cobalamin; via carbonyl oxygen By similarity PIRSR PIRSR000381-2

Sequences

	Sequence		Length	Mass (Da)
	H0PBQ8 [UniParc]. Last modified February 22, 2012. Version 1. Checksum: 1D9635B1B1DDB583	FASTA	1,195	132,540
	10 20 30 40 50 60 MKSAFLDRIH SPDRPVLVFD GAMGTNLQVQ NLTAADFGGA EYEGCNEYLV HTKPEAVATV 70 80 90 100 110 120 HRAFYEAGAD VVETDTFGGT PLVLAEYDLA DQSYYLNKAA AELAKAVAAE FSTPEKPRFV 130 140 150 160 170 180 AGSMGPGTKL PTLGHVDYDS LKDAYVVQVR GLYDGGVDLL LVETCQDVLQ IKAALNAIEQ 190 200 210 220 230 240 VFAEKGDRLP LMVSVTMETM GTMLVGTEMA AALAILEPYP IDILGLNCAT GPDLMKEHVK 250 260 270 280 290 300 YLSEHSPFVV SCIPNAGLPE NVGGQAFYRL TPMELQMSLM HFIEDLGVQV IGGCCGTRPD 310 320 330 340 350 360 HIKALADIAK DLQPKQRQPH YEPSAASIYS TQTYAQENSF LIIGERLNAS GSKKCRDLLN 370 380 390 400 410 420 AEDWDSLVSL AKSQVKEGAQ ILDVNVDYVG RDGVRDMKEL ASRLVNNVTL PLMLDSTEWQ 430 440 450 460 470 480 KMEAGLKVAG GKCILNSTNY EDGEERFYKV LEIAKEYGAG IVIGTIDEDG MGRTADKKFE 490 500 510 520 530 540 IAKRAYEAAI AFGIPATEIF FDPLALPIST GIEEDRENGK ATVDAIRRIR QELPDCHILL 550 560 570 580 590 600 GVSNVSFGLN PAARQVLNSI FLHECMQVGM DAAIVSANKI LPLAKIDPEQ QQVCLDLIYD 610 620 630 640 650 660 RREFEGERCT YDPLTKLTTL FEGKTTKRDK SGDANLPVEE RLKRHIIDGE RLGLEEALNE 670 680 690 700 710 720 ALKLYAPLDI INIYLLDGMK VVGELFGSGQ MQLPFVLQSA QTMKAAVAFL EPHMDKDDSA 730 740 750 760 770 780 DNAKGTFLIA TVKGDVHDIG KNLVDIILSN NGYRVVNLGI KQPVENIIEA YKKHRPDCIA 790 800 810 820 830 840 MSGLLVKSTA FMKENLEVFN QEGITVPVIL GGAALTPKFV HQDCQNTYKG QVIYGKDAFA 850 860 870 880 890 900 DLHFMDKLMP AKNSHNWDDF QGFLGEYATE NGHNVTTDDG AKTNFGIEEE KLIDASEQSR 910 920 930 940 950 960 EPEVIDTVRS EAVDPDLERP VPPFWGTKIL QSSDISLDEV FPLLDLQALF VGQWQFRKPR 970 980 990 1000 1010 1020 EQSREEYEQF LAEKVHPILA EWKGKVMAEN LLHPTVVYGY FPCQSQGNTL LIYDPELVSQ 1030 1040 1050 1060 1070 1080 NNGQIPPDAT AIAKFEFPRQ KSGRRLCIAD FFASKESGIT DVFPLQAVTV GEIATEYARK 1090 1100 1110 1120 1130 1140 LFAGDNYTDY LYFHGMAVQM AEALAEWTHQ RIRQELGFGH LDPDNIRDLL QQRYQGSRYS 1150 1160 1170 1180 1190 FGYPACPNMQ DQYTQLELLQ TERIGLYMDE SEQVYPEQST TAIISYHPAA KYFSA

References

[1]

"Identification of substrain-specific mutations by massively parallel whole-genome resequencing of Synechocystis sp. PCC 6803." Kanesaki Y., Shiwa Y., Tajima N., Suzuki M., Watanabe S., Sato N., Ikeuchi M., Yoshikawa H. DNA Res. 19:67-79(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP012277 Genomic DNA. Translation: BAL33390.1.
RefSeq	YP_005410112.1. NC_017052.1.
3D structure databases
ProteinModelPortal	H0PBQ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAL33390; BAL33390; SYNPCCN_2473.
GeneID	12012190.
KEGG	sys:SYNPCCN_2473.
Phylogenomic databases
KO	K00548.
Family and domain databases
Gene3D	1.10.1240.10. 1 hit. 3.10.196.10. 1 hit. 3.20.20.20. 1 hit. 3.20.20.330. 1 hit. 3.40.50.280. 1 hit.
InterPro	IPR003759. Cbl-bd_cap. IPR006158. Cobalamin-bd. IPR011005. Dihydropteroate_synth-like. IPR011822. MetH. IPR000489. Pterin-binding. IPR003726. S_MeTrfase. IPR004223. VitB12-dep_Met_synth_activ_dom. [Graphical view]
Pfam	PF02310. B12-binding. 1 hit. PF02607. B12-binding_2. 1 hit. PF02965. Met_synt_B12. 1 hit. PF00809. Pterin_bind. 1 hit. PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF000381. MetH. 1 hit.
SMART	SM01018. B12-binding_2. 1 hit. [Graphical view]
SUPFAM	SSF52242. Cbl-bd. 1 hit. SSF51717. DHP_synth_like. 1 hit. SSF56507. Met_synth_B12. 1 hit. SSF47644. Met_synth_Cbl-bd. 1 hit. SSF82282. S_methyl_trans. 1 hit.
TIGRFAMs	TIGR02082. metH. 1 hit.
PROSITE	PS50974. ADOMET_ACTIVATION. 1 hit. PS51332. B12_BINDING. 1 hit. PS51337. B12_BINDING_NTER. 1 hit. PS50970. HCY. 1 hit. PS50972. PTERIN_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H0PBQ8_9SYNC

Accession

Primary (citable) accession number: H0PBQ8

Entry history

Integrated into UniProtKB/TrEMBL:	February 22, 2012
Last sequence update:	February 22, 2012
Last modified:	May 1, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)