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G9I930

- PA2HB_MICTN

UniProt

G9I930 - PA2HB_MICTN

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Protein
Basic phospholipase A2 homolog Tx-beta
Gene
N/A
Organism
Micrurus tener tener (Texas coral snake)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Heterodimer: snake venom phospholipase that potently activates mouse H+-gated sodium channel ASIC1/ACCN2 (acid-sensitive ion channel 1) expressed in Xenopus oocytes. Both alternatively spliced isoforms ASIC1a and ASIC1b are activated, with a higher potency for ASIC1a (EC(50)=9.4 nM) vs ASIC1b (EC(50)=23 nM). The ASIC3/ACCN3 subtype is also sensitive to the heterodimer, but with a lower potency (EC(50)=830 nM). On ASIC2a/ACCN1, the toxin shows a very weak activation, but produces a remarkable potentiation (>100-fold) of protons when the extracellular pH drops below neutrality. The toxin interacts with the extracellular region of the channel, since responses are only observed in the outside-out configuration. In vivo, the heterodimer elicits robust pain-related behavior in mice by activation of ASIC1/ACCN2 channels on capsaicin-sensitive nerve fibers.1 Publication
Monomer: does not have phospholipase A2 activity but may maintain some lipid-binding character from its PLA2 lineage, which could aid in effecting neuronal depolarization.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phospholipase A2 activity Source: InterPro

GO - Biological processi

  1. lipid catabolic process Source: InterPro
  2. phospholipid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 homolog Tx-beta
Short name:
svPLA2 homolog
Alternative name(s):
MitTx-beta
OrganismiMicrurus tener tener (Texas coral snake)
Taxonomic identifieri1114302 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeMicrurus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 30301 Publication
Add
BLAST
Chaini31 – 149119Basic phospholipase A2 homolog Tx-beta
PRO_5000828218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 100 By similarity
Disulfide bondi55 ↔ 148 By similarity
Disulfide bondi57 ↔ 73 By similarity
Disulfide bondi72 ↔ 130 By similarity
Disulfide bondi79 ↔ 123 By similarity
Disulfide bondi89 ↔ 116 By similarity
Disulfide bondi109 ↔ 121 By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chains.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 409
Helixi47 – 493
Turni50 – 523
Turni54 – 563
Helixi68 – 8720
Turni91 – 933
Helixi104 – 1063
Helixi115 – 13319
Helixi138 – 1403
Helixi145 – 1473

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NTWX-ray2.07C31-149[»]
4NTXX-ray2.27C31-149[»]
4NTYX-ray2.65C31-149[»]
ProteinModelPortaliG9I930.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G9I930-1 [UniParc]FASTAAdd to Basket

« Hide

MDKMNPAHLL VLAAVCVSLL GASSIPPQAL NLNQFRLMIK CTNDRVWADF    50
VDYGCYCVAR DSNTPVDDLD RCCQAQKQCY DEAVKVHGCK PLVMFYSFEC 100
RYLASDLDCS GNNTKCRNFV CNCDRTATLC ILTATYNRNN HKIDPSRCQ 149
Length:149
Mass (Da):16,793
Last modified:February 22, 2012 - v1
Checksum:i4CF027CBCA751BE4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JN613326 mRNA. Translation: AET85560.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

The poison in pain - Issue 140 of July 2012

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JN613326 mRNA. Translation: AET85560.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4NTW X-ray 2.07 C 31-149 [» ]
4NTX X-ray 2.27 C 31-149 [» ]
4NTY X-ray 2.65 C 31-149 [» ]
ProteinModelPortali G9I930.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.90.10. 1 hit.
InterProi IPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view ]
PANTHERi PTHR11716. PTHR11716. 1 hit.
Pfami PF00068. Phospholip_A2_1. 1 hit.
[Graphical view ]
PRINTSi PR00389. PHPHLIPASEA2.
SMARTi SM00085. PA2c. 1 hit.
[Graphical view ]
SUPFAMi SSF48619. SSF48619. 1 hit.
PROSITEi PS00119. PA2_ASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A heteromeric Texas coral snake toxin targets acid-sensing ion channels to produce pain."
    Bohlen C.J., Chesler A.T., Sharif-Naeini R., Medzihradszky K.F., Zhou S., King D., Sanchez E.E., Burlingame A.L., Basbaum A.I., Julius D.
    Nature 479:410-414(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, SUBUNIT.
    Tissue: Venom and Venom gland.

Entry informationi

Entry nameiPA2HB_MICTN
AccessioniPrimary (citable) accession number: G9I930
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: February 22, 2012
Last modified: May 14, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This toxin has been studied to probe molecular mechanisms underlying pain sensation. By targeting ASIC1/ACCN2, it reveals an unexpected contribution of this channel to nociception.
The heterodimeric toxin does not affect ASIC2b/ACCN1, ASIC4/ACCN4, Kv2.1/KCNB1, Cav3.3/CACNA1I, ENaC/SCNN1A, TRPA1, TRPV1, TRPV3, TRPM8, P2X2/P2RX2, and 5-HT3/HTR3A channels (1 Publication).

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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