G9AGS6 (G9AGS6_RHIFH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Catalase-peroxidase HAMAP-Rule MF_01961
Short name=CP HAMAP-Rule MF_01961
EC=1.11.1.21 HAMAP-Rule MF_01961

Alternative name(s):
Peroxidase/catalase HAMAP-Rule MF_01961

Gene names

Name:	katG HAMAP-Rule MF_01961
Ordered Locus Names:	SFHH103_05796 EMBL CCF00258.1

Encoded on

Plasmid pSfHH103e EMBL CCF00258.1

Organism

Rhizobium fredii (strain HH103) (Sinorhizobium fredii) [Complete proteome] [HAMAP] EMBL CCF00258.1

Taxonomic identifier

1117943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP-Rule MF_01961
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP-Rule MF_01961 RuleBase RU003451 SAAS SAAS000763 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP-Rule MF_01961 SAAS SAAS000763
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. HAMAP-Rule MF_01961
Subunit structure	Homodimer or homotetramer By similarity. HAMAP-Rule MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP-Rule MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily. HAMAP-Rule MF_01961 RuleBase RU003451

Ontologies

Keywords
Biological process	Hydrogen peroxide HAMAP-Rule MF_01961 RuleBase RU003451 SAAS SAAS000763
Domain	Signal HAMAP-Rule MF_01961
Ligand	Heme HAMAP-Rule MF_01961 RuleBase RU003900 SAAS SAAS000763 Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid EMBL CCF00258.1
Gene Ontology (GO)
Biological_process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity HAMAP-Rule MF_01961
Sites
	Active site	98	1	Proton acceptor By similarity HAMAP-Rule MF_01961
	Metal binding	261	1	Iron (heme axial ligand) By similarity HAMAP-Rule MF_01961
	Site	94	1	Transition state stabilizer By similarity HAMAP-Rule MF_01961
Amino acid modifications
	Cross-link	97 ↔ 220		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with Met-246) By similarity HAMAP-Rule MF_01961
	Cross-link	220 ↔ 246		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with Trp-97) By similarity HAMAP-Rule MF_01961

Sequences

	Sequence		Length	Mass (Da)
	G9AGS6 [UniParc]. Last modified February 22, 2012. Version 1. Checksum: 87293D420A2D8C93	FASTA	732	80,706
	10 20 30 40 50 60 MDSKVESAGK CPVVHTHTAH GGRSNRDWWP NQLNVRILHQ NSSLSDPMGQ AFNYAEEFKK 70 80 90 100 110 120 LDLEALKKDL LALMTDSQDW WPADFGHYGP LFIRMAWHSA GTYRTGDGRG GAGAGQQRFA 130 140 150 160 170 180 PLNSWPDNVN LDKARRLLWP IKQKYGNQIS WADLMILTGN VALESMGFKT FGFAGGRPDV 190 200 210 220 230 240 WEPEEDVYWG AEETWLADKR YTGERDLEKP LAAVQMGLIY VNPEGPNGNP DPLAAAKDIR 250 260 270 280 290 300 ETFARMAMND EETVALIAGG HTFGKTHGAG EATHVGVEPE GAGIEEQGFG WTNSFGTGKG 310 320 330 340 350 360 GDTIGSGLEV TWTTTPTRWS NNFFWNLFGY EWELTKSPAG AHQWTPKHGA GAATVPDAHD 370 380 390 400 410 420 ASKRHAPAML TTDLALRFDP AYEKIARRFF ENPDQFADAF ARAWFKLTHR DMGPRVRYLG 430 440 450 460 470 480 PEVPAEELIW QDPVPAVDHP LIDAQDVADL KDKILASGLP ISHLVSTAWA SASTFRGSDK 490 500 510 520 530 540 RGGANGARIR LAPQKDWEVN QPAQLATVLE TLEGIQKAFN DAQSGGKKVS LADLIVLGGA 550 560 570 580 590 600 AAIEKAARNA GHDIEVPFAP GRTDATQEQT DVDSFGVLEP ISDGFRNYQK GDYLISPEEL 610 620 630 640 650 660 LIDRAQLLTL TAPEMTVLVG GMRALNANVG QVQHGVFTTR PESLTNDFFV NLLDMGTAWK 670 680 690 700 710 720 VSPESKYVFE GRDRNTDEMK WTATRIDLVF GSNSQLRALA EVYGQSDTQE KFVRDFVAAW 730 TKVMNADRFD LV

References

[1]

"Genome sequence of the soybean symbiont Sinorhizobium fredii HH103." Weidner S., Becker A., Bonilla I., Jaenicke S., Lloret J., Margaret I., Puhler A., Ruiz-Sainz J.E., Schneiker-Bekel S., Szczepanowski R., Vinardell J.M., Zehner S., Gottfert M. J. Bacteriol. 194:1617-1618(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HH103 EMBL CCF00258.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE616899 Genomic DNA. Translation: CCF00258.1.
RefSeq	YP_005192397.1. NC_016815.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCF00258; CCF00258; SFHH103_05796.
GeneID	11799986.
KEGG	sfh:SFHH103_05796.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K03782.
Family and domain databases
HAMAP	MF_01961. Catal_peroxid.
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G9AGS6_RHIFH

Accession

Primary (citable) accession number: G9AGS6

Entry history

Integrated into UniProtKB/TrEMBL:	February 22, 2012
Last sequence update:	February 22, 2012
Last modified:	April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)