G9A4N8 (G9A4N8_RHIFH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
Short name=Glu-ADT subunit A HAMAP-Rule MF_00120
EC=6.3.5.- HAMAP-Rule MF_00120

Gene names

Name:	gatA HAMAP-Rule MF_00120 EMBL CCE95532.1
Ordered Locus Names:	SFHH103_01033 EMBL CCE95532.1

Organism

Rhizobium fredii (strain HH103) (Sinorhizobium fredii) [Complete proteome] [HAMAP] EMBL CCE95532.1

Taxonomic identifier

1117943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. SAAS SAAS004412 HAMAP-Rule MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily. HAMAP-Rule MF_00120

Ontologies

Keywords
Biological process	Protein biosynthesis SAAS SAAS004412 HAMAP-Rule MF_00120
Ligand	ATP-binding SAAS SAAS004412 HAMAP-Rule MF_00120 Nucleotide-binding
Molecular function	Ligase SAAS SAAS004412 HAMAP-Rule MF_00120 EMBL CCE95532.1 Transferase EMBL CCE95532.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	122	1	Charge relay system By similarity HAMAP-Rule MF_00120
	Active site	202	1	Charge relay system By similarity HAMAP-Rule MF_00120
	Active site	226	1	Acyl-ester intermediate By similarity HAMAP-Rule MF_00120

Sequences

	Sequence		Length	Mass (Da)
	G9A4N8 [UniParc]. Last modified February 22, 2012. Version 1. Checksum: 994A8020CAFDD63F	FASTA	536	57,445
	10 20 30 40 50 60 MATRPPISSP TRRIRIVISS SFPRSSSKPL PPPASRFRKF DCTMTDLTSL TIAEARAKLS 70 80 90 100 110 120 AKEITAVELT DAYIAAIEAA NDTLNAYVTV TPEKAREMAK ASDARIAAGK AGALEGIPLG 130 140 150 160 170 180 IKDLFGTEGV HTQACSHILD GFKPRYESTV TQNLWNDGAV MLGKLNMDEF AMGSSNETSY 190 200 210 220 230 240 YGPVKNPWRA KGSNMDLVPG GSSGGSAAAV AAYLCAGATA TDTGGSIRQP AAFTGTVGIK 250 260 270 280 290 300 PTYGRCSRWG VVAFASSLDQ AGPIARDVRD AAILLTSMAS VDPKDTTSVD IPVPDYEASL 310 320 330 340 350 360 GQSIKGMKIG IPKEYRVDGM PEEIEALWQQ GIAWLKEAGA EIVDITLPHT KYALPAYYIV 370 380 390 400 410 420 APAEASSNLA RYDGVRYGLR VDGKDIVDMY EKTRAAGFGR EVKRRIMIGT YVLSAGYYDA 430 440 450 460 470 480 YYLRAQKVRT LIKRDFELAF QAGVDAILTP ATPSSAFGIA DEDLASDPVK MYLNDIFTVT 490 500 510 520 530 VNMAGLPGIA VPGGLDHKGL PLGLQLIGKP FDEETLFKTA HVIEQAAGRF APSKWW

References

[1]

"Genome sequence of the soybean symbiont Sinorhizobium fredii HH103." Weidner S., Becker A., Bonilla I., Jaenicke S., Lloret J., Margaret I., Puhler A., Ruiz-Sainz J.E., Schneiker-Bekel S., Szczepanowski R., Vinardell J.M., Zehner S., Gottfert M. J. Bacteriol. 194:1617-1618(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HH103 EMBL CCE95532.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE616890 Genomic DNA. Translation: CCE95532.1.
RefSeq	YP_005188357.1. NC_016812.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCE95532; CCE95532; SFHH103_01033.
GeneID	11813231.
KEGG	sfh:SFHH103_01033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K02433.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. gatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G9A4N8_RHIFH

Accession

Primary (citable) accession number: G9A4N8

Entry history

Integrated into UniProtKB/TrEMBL:	February 22, 2012
Last sequence update:	February 22, 2012
Last modified:	April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)